MLK4 Antibody - #AF0596
(2)
| Product: | MLK4 Antibody |
| Catalog: | AF0596 |
| Description: | Rabbit polyclonal antibody to MLK4 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human |
| Mol.Wt.: | 110kDa; 114kD(Calculated). |
| Uniprot: | Q5TCX8 |
| RRID: | AB_2834282 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human
Clonality:
Polyclonal
Specificity:
MLK4 Antibody detects endogenous levels of total MLK4.
RRID:
AB_2834282
Cite Format: Affinity Biosciences Cat# AF0596, RRID:AB_2834282.
Cite Format: Affinity Biosciences Cat# AF0596, RRID:AB_2834282.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
dJ862P8.3; KIAA1804; M3KL4_HUMAN; Mitogen activated protein kinase kinase kinase; Mitogen-activated protein kinase kinase kinase MLK4; Mixed lineage kinase 4; MLK 4; Mlk4; RP5 862P8.2;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Description:
MLK4 Activates the JUN N-terminal pathway. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Homodimer. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Sequence:
- Q5TCX8 M3K21_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALRGAAGATDTPVSSAGGAPGGSASSSSTSSGGSASAGAGLWAALYDYEARGEDELSLRRGQLVEVLSQDAAVSGDEGWWAGQVQRRLGIFPANYVAPCRPAASPAPPPSRPSSPVHVAFERLELKELIGAGGFGQVYRATWQGQEVAVKAARQDPEQDAAAAAESVRREARLFAMLRHPNIIELRGVCLQQPHLCLVLEFARGGALNRALAAANAAPDPRAPGPRRARRIPPHVLVNWAVQIARGMLYLHEEAFVPILHRDLKSSNILLLEKIEHDDICNKTLKITDFGLAREWHRTTKMSTAGTYAWMAPEVIKSSLFSKGSDIWSYGVLLWELLTGEVPYRGIDGLAVAYGVAVNKLTLPIPSTCPEPFAKLMKECWQQDPHIRPSFALILEQLTAIEGAVMTEMPQESFHSMQDDWKLEIQQMFDELRTKEKELRSREEELTRAALQQKSQEELLKRREQQLAEREIDVLERELNILIFQLNQEKPKVKKRKGKFKRSRLKLKDGHRISLPSDFQHKITVQASPNLDKRRSLNSSSSSPPSSPTMMPRLRAIQLTSDESNKTWGRNTVFRQEEFEDVKRNFKKKGCTWGPNSIQMKDRTDCKERIRPLSDGNSPWSTILIKNQKTMPLASLFVDQPGSCEEPKLSPDGLEHRKPKQIKLPSQAYIDLPLGKDAQRENPAEAESWEEAASANAATVSIEMTPTNSLSRSPQRKKTESALYGCTVLLASVALGLDLRELHKAQAAEEPLPKEEKKKREGIFQRASKSRRSASPPTSLPSTCGEASSPPSLPLSSALGILSTPSFSTKCLLQMDSEDPLVDSAPVTCDSEMLTPDFCPTAPGSGREPALMPRLDTDCSVSRNLPSSFLQQTCGNVPYCASSKHRPSHHRRTMSDGNPTPTGATIISATGASALPLCPSPAPHSHLPREVSPKKHSTVHIVPQRRPASLRSRSDLPQAYPQTAVSQLAQTACVVGRPGPHPTQFLAAKERTKSHVPSLLDADVEGQSRDYTVPLCRMRSKTSRPSIYELEKEFLS
PTMs - Q5TCX8 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y47 | Phosphorylation | Uniprot | |
| Y96 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| T142 | Phosphorylation | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| K454 | Ubiquitination | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| S514 | Phosphorylation | Uniprot | |
| S517 | Phosphorylation | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| S542 | Phosphorylation | Uniprot | |
| S543 | Phosphorylation | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| S561 | Phosphorylation | Uniprot | |
| T592 | Phosphorylation | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| T604 | Phosphorylation | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| S618 | Phosphorylation | Uniprot | |
| S621 | Phosphorylation | Uniprot | |
| T622 | Phosphorylation | Uniprot | |
| S650 | Phosphorylation | Uniprot | |
| Y669 | Phosphorylation | Uniprot | |
| T719 | Phosphorylation | Uniprot | |
| Y724 | Phosphorylation | Uniprot | |
| S768 | Phosphorylation | Uniprot | |
| S773 | Phosphorylation | Uniprot | |
| S895 | Phosphorylation | Uniprot | |
| S920 | Phosphorylation | Uniprot | |
| S932 | Phosphorylation | Uniprot | |
| S949 | Phosphorylation | Uniprot | |
| S1026 | Phosphorylation | Uniprot |
PTMs - Q5TCX8 As Enzyme
Research Backgrounds
Function:
Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways.
PTMs:
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
Subunit Structure:
Homodimer (By similarity). Interacts with TLR4.
Family&Domains:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
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