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$280 100ul
$350 200ul

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  • Product Name
    MLK4 Antibody
  • Catalog No.
    AF0596
  • RRID
    AB_2834282
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA(peptide)
  • Reactivity
    Human
  • UniProt
  • Mol.Wt
    110kD;
    114kDa(Calculated).
  • Concentration
    1mg/ml
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Product Information

Alternative Names:Expand▼

dJ862P8.3; KIAA1804; M3KL4_HUMAN; Mitogen activated protein kinase kinase kinase; Mitogen-activated protein kinase kinase kinase MLK4; Mixed lineage kinase 4; MLK 4; Mlk4; RP5 862P8.2;

Applications:

WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

MLK4 Antibody detects endogenous levels of total MLK4.

RRID:

AB_2834282
Please cite this product as: Affinity Biosciences Cat# AF0596, RRID:AB_2834282.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human MLK4, corresponding to a region within C-terminal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

MAP3K21

Molecular Weight:

Observed Mol.Wt.: 110kD.
Predicted Mol.Wt.: 114kDa(Calculated)..

Description:

MLK4 Activates the JUN N-terminal pathway. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Homodimer. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.

Sequence:
MALRGAAGATDTPVSSAGGAPGGSASSSSTSSGGSASAGAGLWAALYDYEARGEDELSLRRGQLVEVLSQDAAVSGDEGWWAGQVQRRLGIFPANYVAPCRPAASPAPPPSRPSSPVHVAFERLELKELIGAGGFGQVYRATWQGQEVAVKAARQDPEQDAAAAAESVRREARLFAMLRHPNIIELRGVCLQQPHLCLVLEFARGGALNRALAAANAAPDPRAPGPRRARRIPPHVLVNWAVQIARGMLYLHEEAFVPILHRDLKSSNILLLEKIEHDDICNKTLKITDFGLAREWHRTTKMSTAGTYAWMAPEVIKSSLFSKGSDIWSYGVLLWELLTGEVPYRGIDGLAVAYGVAVNKLTLPIPSTCPEPFAKLMKECWQQDPHIRPSFALILEQLTAIEGAVMTEMPQESFHSMQDDWKLEIQQMFDELRTKEKELRSREEELTRAALQQKSQEELLKRREQQLAEREIDVLERELNILIFQLNQEKPKVKKRKGKFKRSRLKLKDGHRISLPSDFQHKITVQASPNLDKRRSLNSSSSSPPSSPTMMPRLRAIQLTSDESNKTWGRNTVFRQEEFEDVKRNFKKKGCTWGPNSIQMKDRTDCKERIRPLSDGNSPWSTILIKNQKTMPLASLFVDQPGSCEEPKLSPDGLEHRKPKQIKLPSQAYIDLPLGKDAQRENPAEAESWEEAASANAATVSIEMTPTNSLSRSPQRKKTESALYGCTVLLASVALGLDLRELHKAQAAEEPLPKEEKKKREGIFQRASKSRRSASPPTSLPSTCGEASSPPSLPLSSALGILSTPSFSTKCLLQMDSEDPLVDSAPVTCDSEMLTPDFCPTAPGSGREPALMPRLDTDCSVSRNLPSSFLQQTCGNVPYCASSKHRPSHHRRTMSDGNPTPTGATIISATGASALPLCPSPAPHSHLPREVSPKKHSTVHIVPQRRPASLRSRSDLPQAYPQTAVSQLAQTACVVGRPGPHPTQFLAAKERTKSHVPSLLDADVEGQSRDYTVPLCRMRSKTSRPSIYELEKEFLS

Research Background

Function:

Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways.

Post-translational Modifications:

Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.

Subunit Structure:

Homodimer (By similarity). Interacts with TLR4.

Similarity:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

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Catalog Number :

AF0596-BP
(Blocking peptide available as AF0596-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
75%
Horse
75%
Bovine
67%
Sheep
58%
Dog
0%
Xenopus
0%
Zebrafish
0%
Chicken
0%
Rabbit
0%
High similarity Medium similarity Low similarity No similarity
Q5TCX8 as Substrate
Site PTM Type Enzyme
Y47 Phosphorylation
Y96 Phosphorylation
S105 Phosphorylation
S111 Phosphorylation
S114 Phosphorylation
S115 Phosphorylation
T142 Phosphorylation
K286 Ubiquitination
K454 Ubiquitination
S455 Phosphorylation
S514 Phosphorylation
S517 Phosphorylation
S528 Phosphorylation
S542 Phosphorylation
S543 Phosphorylation
S546 Phosphorylation
S561 Phosphorylation
T592 Phosphorylation
S597 Phosphorylation
T604 Phosphorylation
S614 Phosphorylation
S618 Phosphorylation
S621 Phosphorylation
T622 Phosphorylation
S650 Phosphorylation
Y669 Phosphorylation
T719 Phosphorylation
Y724 Phosphorylation
S768 Phosphorylation
S773 Phosphorylation
S895 Phosphorylation
S920 Phosphorylation
S932 Phosphorylation
S949 Phosphorylation
S1026 Phosphorylation
Q5TCX8 as PTM Enzyme
Substrate Site Source
Q02750 (MAP2K1) S218 Uniprot
Q02750 (MAP2K1) S222 Uniprot
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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