Product: Phospho-HMG17 (Ser29) Antibody
Catalog: AF8065
Description: Rabbit polyclonal antibody to Phospho-HMG17 (Ser29)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Dog, Chicken, Xenopus
Mol.Wt.: 9 kDa; 9kD(Calculated).
Uniprot: P05204
RRID: AB_2840128

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 100ul $350 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-HMG17 (Ser29) Antibody detects endogenous levels of HMG17 only when phosphorylated at Ser29.
RRID:
AB_2840128
Cite Format: Affinity Biosciences Cat# AF8065, RRID:AB_2840128.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

High mobility group (nonhistone chromosomal) protein 17; high mobility group nucleosomal binding domain 2; High mobility group nucleosome-binding domain-containing protein 2; High mobility group protein N2; HMG17; HMGN2; HMGN2_HUMAN; MGC5629; Non histone chromosomal protein HMG 17; Non-histone chromosomal protein HMG-17; Nonhistone chromosomal protein HMG 17; nonhistone chromosomal protein hmg-17;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MPKRKAEGDAKGDKAKVKDEPQRRSARLSAKPAPPKPEPKPKKAPAKKGEKVPKGKKGKADAGKEGNNPAENGDAKTDQAQKAEGAGDAK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Horse
0
Sheep
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05204 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K3 Acetylation
K5 Acetylation
K11 Acetylation
K14 Acetylation
K18 Sumoylation
S25 Phosphorylation Q96GD4 (AURKB) , P17252 (PRKCA) , P17612 (PRKACA) , P51812 (RPS6KA3)
S29 Phosphorylation P17252 (PRKCA) , Q96GD4 (AURKB) , P51812 (RPS6KA3) , P17612 (PRKACA)
K31 Acetylation
K31 Ubiquitination
K36 Acetylation
K36 Sumoylation
K36 Ubiquitination
K40 Acetylation
K40 Ubiquitination
K47 Ubiquitination
K48 Ubiquitination
K51 Ubiquitination
K54 Acetylation
K56 Acetylation
K57 Acetylation
K59 Acetylation
K64 Acetylation
K64 Ubiquitination
K76 Acetylation
K76 Ubiquitination
K82 Acetylation
K82 Sumoylation
K82 Ubiquitination
K90 Acetylation

Research Backgrounds

Function:

Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation (By similarity).

PTMs:

Phosphorylation favors cytoplasmic localization.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Cytoplasmic enrichment upon phosphorylation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the HMGN family.

Restrictive clause

 

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For Research Use Only.
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