Phospho-Centaurin beta-1 (Ser554) Antibody

WB
pElisa

1/2

Western blot analysis of extracts from COLO205 cells(heat-shock treatment), using Phospho-Centaurin beta-1 (Ser554) Antibody at 1/1000 dilution.

peptide-ELISA analysis of AF8083.

Product:	Phospho-Centaurin beta-1 (Ser554) Antibody
Catalog:	AF8083
Description:	Rabbit polyclonal antibody to Phospho-Centaurin beta-1 (Ser554)
Application:	WB
Reactivity:	Human, Mouse
Mol.Wt.:	81KD; 82kD(Calculated).
Uniprot:	Q15027
RRID:	AB_2840146

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

Phospho-Centaurin beta-1 (Ser554) Antibody detects endogenous levels of Centaurin beta-1 only when phosphorylated at Ser554.

RRID:

AB_2840146
Cite Format: Affinity Biosciences Cat# AF8083, RRID:AB_2840146.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACAP 1; ACAP1; ACAP1_HUMAN; Arf GAP with coiled coil ANK repeat and PH domains 1; Arf GAP with coiled coil, ankyrin repeat, and pleckstrin homology domains 1; Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1; ArfGAP with coiled-coil, ankyrin repeat and PH domains 1; CENT B1; Centaurin beta 1; Centaurin-beta-1; CENTB 1; Cnt b1; Cnt-b1; Cntb1; KIAA0050;

Immunogens

Immunogen:

Uniprot:

Q15027(ACAP1_HUMAN) >>Visit HPA database.

Gene(ID):

ACAP1(9744)

Expression:

Q15027 ACAP1_HUMAN:

Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.

Sequence:

Q15027 ACAP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTVKLDFEECLKDSPRFRASIELVEAEVSELETRLEKLLKLGTGLLESGRHYLAASRAFVVGICDLARLGPPEPMMAECLEKFTVSLNHKLDSHAELLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTHNAEVPRRRAQEAEEAGAALRTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLSQYRKELGAQLHQLVLNSAREKRDMEQRHVLLKQKELGGEEPEPSLREGPGGLVMEGHLFKRASNAFKTWSRRWFTIQSNQLVYQKKYKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSTSKSCLLQADSERLLQLWVSAVQSSIASAFSQARLDDSPRGPGQGSGHLAIGSAATLGSGGMARGREPGGVGHVVAQVQSVDGNAQCCDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVIINQIYEARVEAMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGRPRGQPPVPPKPSIRPRPGSLRSKPEPPSEDLGSLHPGALLFRASGHPPSLPTMADALAHGADVNWVNGGQDNATPLIQATAANSLLACEFLLQNGANVNQADSAGRGPLHHATILGHTGLACLFLKRGADLGARDSEGRDPLTIAMETANADIVTLLRLAKMREAEAAQGQAGDETYLDIFRDFSLMASDDPEKLSRRSHDLHTL

PTMs - Q15027 As Substrate

Q15027

Site	PTM Type	Enzyme	Source
K4	Acetylation		Uniprot
K4	Ubiquitination		Uniprot
K12	Ubiquitination		Uniprot
S14	Phosphorylation		Uniprot
K37	Ubiquitination		Uniprot
K40	Ubiquitination		Uniprot
T43	Phosphorylation		Uniprot
K82	Ubiquitination		Uniprot
K90	Ubiquitination		Uniprot
K114	Ubiquitination		Uniprot
S133	Phosphorylation		Uniprot
K183	Ubiquitination		Uniprot
K185	Ubiquitination		Uniprot
K218	Ubiquitination		Uniprot
K246	Ubiquitination		Uniprot
K248	Ubiquitination		Uniprot
K274	Acetylation		Uniprot
K274	Ubiquitination		Uniprot
S277	Phosphorylation		Uniprot
K281	Ubiquitination		Uniprot
Y297	Phosphorylation		Uniprot
K302	Ubiquitination		Uniprot
T306	Phosphorylation		Uniprot
K318	Ubiquitination		Uniprot
S333	Phosphorylation		Uniprot
S371	Phosphorylation		Uniprot
S386	Phosphorylation		Uniprot
T389	Phosphorylation		Uniprot
S392	Phosphorylation		Uniprot
K456	Ubiquitination		Uniprot
K495	Ubiquitination		Uniprot
K496	Ubiquitination		Uniprot
K506	Ubiquitination		Uniprot
K513	Ubiquitination		Uniprot
K522	Ubiquitination		Uniprot
K545	Ubiquitination		Uniprot
S547	Phosphorylation		Uniprot
S554	Phosphorylation	P31749 (AKT1)	Uniprot
K558	Ubiquitination		Uniprot
T711	Phosphorylation		Uniprot
Y712	Phosphorylation		Uniprot
S720	Phosphorylation		Uniprot
K729	Ubiquitination		Uniprot
S731	Phosphorylation		Uniprot
T739	Phosphorylation		Uniprot

Research Backgrounds

Q15027_ACAP1_HUMAN

Function:

GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.

PTMs:

Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.

Subcellular Location:

Recycling endosome membrane>Peripheral membrane protein>Cytoplasmic side.

Tissue Specificity:

Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.

Subunit Structure:

Banana-shaped homodimer laterally assembling into tetramers, the tetramers further pack helically onto the membrane. Interacts with GTP-bound ARF6. Interacts with third cytoplasmic loop of SLC2A4/GLUT4. Interacts with CLTC. Interacts with GULP1. Forms a complex with GDP-bound ARF6 and GULP1. Interacts with ITGB1; required for ITGB1 recycling.

Family&Domains:

PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes. Only one PH domain of one ACAP1 dimer inserts into the membrane, while the other PH domain acts primaryly to interact with adjacent ACAP1 dimers.

The BAR domain mediates homodimerization, it can neither bind membrane nor impart curvature, but instead requires the neighboring PH domain to achieve these functions.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

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Phospho-Centaurin beta-1 (Ser554) Antibody - #AF8083