MAP3K3 Antibody | Affinity Biosciences

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Western blot analysis of extracts from 293 and Hela , using MAP3K3 Antibody.

Product:	MAP3K3 Antibody
Catalog:	AF0449
Description:	Rabbit polyclonal antibody to MAP3K3
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	71kDa; 71kD(Calculated).
Uniprot:	Q99759
RRID:	AB_2834305

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(82%)

Clonality:

Polyclonal

Specificity:

MAP3K3 Antibody detects endogenous levels of total MAP3K3.

RRID:

AB_2834305
Cite Format: Affinity Biosciences Cat# AF0449, RRID:AB_2834305.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

M3K3_HUMAN; MAP/ERK kinase kinase 3; MAP3K3; MAPK/ERK kinase kinase 3; MAPKKK3; MEK kinase 3; MEKK 3; MEKK3; Mitogen activated protein kinase kinase kinase 3; Mitogen-activated protein kinase kinase kinase 3;

Immunogens

Immunogen:

Uniprot:

Q99759(M3K3_HUMAN) >>Visit HPA database.

Gene(ID):

MAP3K3(4215)

Description:

This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Sequence:

Q99759 M3K3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDEQEALNSIMNDLVALQMNRRHRMPGYETMKNKDTGHSNRQSDVRIKFEHNGERRIIAFSRPVKYEDVEHKVTTVFGQPLDLHYMNNELSILLKNQDDLDKAIDILDRSSSMKSLRILLLSQDRNHNSSSPHSGVSRQVRIKASQSAGDINTIYQPPEPRSRHLSVSSQNPGRSSPPPGYVPERQQHIARQGSYTSINSEGEFIPETSEQCMLDPLSSAENSLSGSCQSLDRSADSPSFRKSRMSRAQSFPDNRQEYSDRETQLYDKGVKGGTYPRRYHVSVHHKDYSDGRRTFPRIRRHQGNLFTLVPSSRSLSTNGENMGLAVQYLDPRGRLRSADSENALSVQERNVPTKSPSAPINWRRGKLLGQGAFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNLQHERIVQYYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRRIFVEARQRPSAEELLTHHFAQLMY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Zebrafish

Chicken

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99759 As Substrate

Q99759

Site	PTM Type	Enzyme	Source
K102	Ubiquitination		Uniprot
S130	Phosphorylation		Uniprot
S131	Phosphorylation		Uniprot
S145	Phosphorylation		Uniprot
S147	Phosphorylation		Uniprot
Y155	Phosphorylation		Uniprot
S162	Phosphorylation		Uniprot
S166	Phosphorylation	O00141 (SGK1)	Uniprot
S168	Phosphorylation		Uniprot
S169	Phosphorylation		Uniprot
S175	Phosphorylation		Uniprot
S176	Phosphorylation		Uniprot
S194	Phosphorylation		Uniprot
S234	Phosphorylation		Uniprot
S237	Phosphorylation		Uniprot
S250	Phosphorylation		Uniprot
T263	Phosphorylation		Uniprot
Y266	Phosphorylation		Uniprot
T274	Phosphorylation		Uniprot
Y275	Phosphorylation		Uniprot
Y288	Phosphorylation		Uniprot
S289	Phosphorylation		Uniprot
T294	Phosphorylation		Uniprot
S312	Phosphorylation		Uniprot
S314	Phosphorylation		Uniprot
S316	Phosphorylation		Uniprot
T317	Phosphorylation		Uniprot
S337	Phosphorylation	O00141 (SGK1)	Uniprot
S340	Phosphorylation		Uniprot
S345	Phosphorylation		Uniprot
S355	Phosphorylation		Uniprot
S357	Phosphorylation		Uniprot
K366	Ubiquitination		Uniprot
K391	Ubiquitination		Uniprot
K404	Ubiquitination		Uniprot
K417	Ubiquitination		Uniprot
K456	Ubiquitination		Uniprot
S464	Phosphorylation		Uniprot
K491	Ubiquitination		Uniprot
K504	Ubiquitination		Uniprot
K512	Ubiquitination		Uniprot
T516	Phosphorylation		Uniprot
S520	Phosphorylation		Uniprot
S526	Phosphorylation	Q99759 (MAP3K3)	Uniprot
T528	Phosphorylation		Uniprot
T530	Phosphorylation		Uniprot

PTMs - Q99759 As Enzyme

Q99759

Substrate	Site	Source
P46734 (MAP2K3)	S218	Uniprot
P53805 (RCAN1)	S163	Uniprot
P53805 (RCAN1)	S167	Uniprot
Q99759-1 (MAP3K3)	S526	Uniprot
Q99759-2 (MAP3K3)	S557	Uniprot

Research Backgrounds

Q99759_M3K3_HUMAN

Function:

Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.

PTMs:

Phosphorylation at Ser-166 and Ser-337 by SGK1 inhibits its activity.

Subunit Structure:

Binds both upstream activators and downstream substrates in multimolecular complexes. Part of a complex with MAP2K3, RAC1 and CCM2. Interacts with MAP2K5 and SPAG9.

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)

Restrictive clause

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For Research Use Only.
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MAP3K3 Antibody - #AF0449