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$450 200ul

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  • Product Name
    Phospho-ZAK (Ser165) Antibody
  • Catalog No.
    AF8195
  • Source
    Rabbit
  • Application
    WB,ELISA
  • Reactivity:
    Human, Mouse
  • Prediction:
    Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
  • UniProt
  • Mol.Wt.
    91 KD
  • Concentration
    1mg/ml
  • Browse similar products>>

Product Information

Alternative Names:Expand▼

AZK; Cervical cancer suppressor gene 4 protein; HCCS 4; HCCS-4; HCCS4; Human cervical cancer suppressor gene 4 protein; Leucine zipper and sterile alpha motif containing kinase; Leucine zipper- and sterile alpha motif-containing kinase; Mitogen activated protein kinase kinase kinase MLT; Mitogen-activated protein kinase kinase kinase MLT; Mixed lineage kinase 7; Mixed lineage kinase related kinase; Mixed lineage kinase related kinase MRK beta; Mixed lineage kinase with a leucine zipper and a sterile alpha motif; Mixed lineage kinase-related kinase; MLK 7; MLK like mitogen activated protein triple kinase; MLK related kinase; MLK-like mitogen-activated protein triple kinase; MLK-related kinase; MLK7; Mlklak; MLT; MLTK_HUMAN; MRK; Sterile alpha motif and leucine zipper containing kinase AZK; Sterile alpha motif- and leucine zipper-containing kinase AZK; Zak;

Applications:

WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Reactivity:

Human, Mouse

Predicted Reactivity:

Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

ZAK (Phospho-Ser165) Antibody detects endogenous levels of ZAK only when phosphorylated at Ser165.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human ZAK around the phosphorylation site of Ser165.

Uniprot:



>>Visit The Human Protein Atlas

Gene id:

Molecular Weight:

Observed Mol.Wt.: 91 KD.
Predicted Mol.Wt.: 92kDa.

Subcellular Location:

Cytoplasm. Nucleus. Translocates to the nucleus upon ultraviolet B irradiation.

Tissue Specificity:

Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed.

Sequence:
        10         20         30         40         50
MSSLGASFVQ IKFDDLQFFE NCGGGSFGSV YRAKWISQDK EVAVKKLLKI
60 70 80 90 100
EKEAEILSVL SHRNIIQFYG VILEPPNYGI VTEYASLGSL YDYINSNRSE
110 120 130 140 150
EMDMDHIMTW ATDVAKGMHY LHMEAPVKVI HRDLKSRNVV IAADGVLKIC
160 170 180 190 200
DFGASRFHNH TTHMSLVGTF PWMAPEVIQS LPVSETCDTY SYGVVLWEML
210 220 230 240 250
TREVPFKGLE GLQVAWLVVE KNERLTIPSS CPRSFAELLH QCWEADAKKR
260 270 280 290 300
PSFKQIISIL ESMSNDTSLP DKCNSFLHNK AEWRCEIEAT LERLKKLERD
310 320 330 340 350
LSFKEQELKE RERRLKMWEQ KLTEQSNTPL LPSFEIGAWT EDDVYCWVQQ
360 370 380 390 400
LVRKGDSSAE MSVYASLFKE NNITGKRLLL LEEEDLKDMG IVSKGHIIHF
410 420 430 440 450
KSAIEKLTHD YINLFHFPPL IKDSGGEPEE NEEKIVNLEL VFGFHLKPGT
460 470 480 490 500
GPQDCKWKMY MEMDGDEIAI TYIKDVTFNT NLPDAEILKM TKPPFVMEKW
510 520 530 540 550
IVGIAKSQTV ECTVTYESDV RTPKSTKHVH SIQWSRTKPQ DEVKAVQLAI
560 570 580 590 600
QTLFTNSDGN PGSRSDSSAD CQWLDTLRMR QIASNTSLQR SQSNPILGSP
610 620 630 640 650
FFSHFDGQDS YAAAVRRPQV PIKYQQITPV NQSRSSSPTQ YGLTKNFSSL
660 670 680 690 700
HLNSRDSGFS SGNTDTSSER GRYSDRSRNK YGRGSISLNS SPRGRYSGKS
710 720 730 740 750
QHSTPSRGRY PGKFYRVSQS ALNPHQSPDF KRSPRDLHQP NTIPGMPLHP
760 770 780 790 800
ETDSRASEED SKVSEGGWTK VEYRKKPHRP SPAKTNKERA RGDHRGWRNF

Background

Function:

Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2 (PubMed:10924358, PubMed:11836244, PubMed:15342622, PubMed:21224381). Involved in limb development (PubMed:26755636).

Subcellular Location:

Nucleus;

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Homodimer (PubMed:10924358, PubMed:26755636). Interacts with PKN1 and ZNF33A (PubMed:12535642, PubMed:12761180). Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, MAP3K20 and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and MAP3K20 (PubMed:21224381).

Similarity:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Research Fields

Research Fields:

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.(View pathway)

Western blot analysis of ZAK (Phospho-Ser165) using HeLa whole cell lysates. -/+ means absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide).
ELISA analysis of AF8195 showing specificity to Phospho-ZAK (Ser165) peptide. Peptides concentration: 1ug/ml.
P-peptide: phospho-peptide; N-peptide: non-phospho-peptide.
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Catalog Number :

AF8195-BP

Price/Size :

$200/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Rabbit
100%
Sheep
100%
Dog
100%
Bovine
100%
Chicken
88%
Horse
0%
Zebrafish
0%
Xenopus
0%
High similarity Medium similarity Low similarity No similarity
Q9NYL2 as Substrate
Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S3 Phosphorylation
S7 Phosphorylation
K34 Ubiquitination
K52 Ubiquitination
Y78 Phosphorylation
Y84 Phosphorylation
Y93 Phosphorylation
K116 Acetylation
Y120 Phosphorylation
K128 Ubiquitination
S136 Phosphorylation
S155 Phosphorylation
T161 Phosphorylation Q9NYL2 (MAP3K20)
T162 Phosphorylation Q9NYL2 (MAP3K20)
S165 Phosphorylation Q9NYL2 (MAP3K20)
K248 Ubiquitination
T267 Phosphorylation
S268 Phosphorylation
K272 Ubiquitination
S275 Phosphorylation
S302 Phosphorylation
K304 Ubiquitination
K309 Ubiquitination
K316 Ubiquitination
K354 Acetylation
S362 Phosphorylation
S366 Phosphorylation
K369 Acetylation
K376 Acetylation
K401 Ubiquitination
K538 Acetylation
K544 Acetylation
S557 Phosphorylation
S563 Phosphorylation
S565 Phosphorylation
S567 Phosphorylation
S568 Phosphorylation
T576 Phosphorylation
S584 Phosphorylation
T586 Phosphorylation
S591 Phosphorylation
S593 Phosphorylation
S599 Phosphorylation
S603 Phosphorylation
S610 Phosphorylation
T628 Phosphorylation
S633 Phosphorylation
S635 Phosphorylation
S636 Phosphorylation
S637 Phosphorylation
T639 Phosphorylation
S648 Phosphorylation
S649 Phosphorylation
S654 Phosphorylation
S660 Phosphorylation
S661 Phosphorylation
T666 Phosphorylation
S667 Phosphorylation
S668 Phosphorylation
R670 Methylation
Y673 Phosphorylation
S674 Phosphorylation
R683 Methylation
S685 Phosphorylation
S687 Phosphorylation
S690 Phosphorylation
S691 Phosphorylation
R693 Methylation
T704 Phosphorylation
R707 Methylation
R709 Methylation
K713 Methylation
S718 Phosphorylation
S720 Phosphorylation
S727 Phosphorylation
S733 Phosphorylation
S757 Phosphorylation
S761 Phosphorylation
S764 Phosphorylation
T769 Phosphorylation
K770 Ubiquitination
S781 Phosphorylation
Q9NYL2 as PTM Enzyme
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.