Product: Phospho-PRK1/2 (Thr774/Thr816) Antibody
Catalog: AF8349
Description: Rabbit polyclonal antibody to Phospho-PRK1/2 (Thr774/Thr816)
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Sheep, Dog, Xenopus
Mol.Wt.: 104KD; 104kD,112kD(Calculated).
Uniprot: Q16512 | Q16513
RRID: AB_2840411

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Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(88%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-PRK1/2 (Thr774/Thr816) Antibody detects endogenous levels of PRK1/2 only when phosphorylated at Thr774/816.
RRID:
AB_2840411
Cite Format: Affinity Biosciences Cat# AF8349, RRID:AB_2840411.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DBK; PAK 1; PAK-1; PAK1; PKC1; PKN ALPHA; PKN; Pkn1; PKN1_HUMAN; PRK1; PRKCL1; Protease activated kinase 1; Protease-activated kinase 1; Protein kinase C like 1; Protein kinase C like PKN; Protein kinase C related kinase 1; Protein kinase C-like 1; Protein kinase C-like PKN; Protein kinase N1; Protein kinase PKN alpha; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; Serine threonine kinase N; Serine threonine protein kinase N; Serine-threonine protein kinase N; Serine/threonine protein kinase N1; Serine/threonine-protein kinase N1; Cardiolipin activated protein kinase Pak2; MGC150606; MGC71074; PAK 2; PAK2; PKN gamma; PKN2; PKN2_HUMAN; PRK2; PRKCL2; PRO2042; Protein kinase C like 2; Protein kinase C related kinase 2; Protein kinase C-like 2; Protein kinase N2; Protein-kinase C-related kinase 2; Serine/threonine-protein kinase N2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q16512 PKN1_HUMAN:

Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells.

Q16513 PKN2_HUMAN:

Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells.

Sequence:
MASDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLRREIRKELKLKEGAENLRRATTDLGRSLGPVELLLRGSSRRLDLLHQQLQELHAHVVLPDPAATHDGPQSPGAGGPTCSATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQAGQLENQAAPDDTQGSPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLREALERRLGELPADHPKGRLLREELAAASSAAFSTRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCRDLPETIPWNPTPSMGGPGTPDSRPPFLSRPARGLYSRSGSLSGRSSLKAEAENTSEVSTVLKLDNTVVGQTSWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFLDFDFVAGGC

MASNPERGEILLTELQGDSRSLPFSENVSAVQKLDFSDTMVQQKLDDIKDRIKREIRKELKIKEGAENLRKVTTDKKSLAYVDNILKKSNKKLEELHHKLQELNAHIVVSDPEDITDCPRTPDTPNNDPRCSTSNNRLKALQKQLDIELKVKQGAENMIQMYSNGSSKDRKLHGTAQQLLQDSKTKIEVIRMQILQAVQTNELAFDNAKPVISPLELRMEELRHHFRIEFAVAEGAKNVMKLLGSGKVTDRKALSEAQARFNESSQKLDLLKYSLEQRLNEVPKNHPKSRIIIEELSLVAASPTLSPRQSMISTQNQYSTLSKPAALTGTLEVRLMGCQDILENVPGRSKATSVALPGWSPSETRSSFMSRTSKSKSGSSRNLLKTDDLSNDVCAVLKLDNTVVGQTSWKPISNQSWDQKFTLELDRSRELEISVYWRDWRSLCAVKFLRLEDFLDNQRHGMCLYLEPQGTLFAEVTFFNPVIERRPKLQRQKKIFSKQQGKTFLRAPQMNINIATWGRLVRRAIPTVNHSGTFSPQAPVPTTVPVVDVRIPQLAPPASDSTVTKLDFDLEPEPPPAPPRASSLGEIDESSELRVLDIPGQDSETVFDIQNDRNSILPKSQSEYKPDTPQSGLEYSGIQELEDRRSQQRFQFNLQDFRCCAVLGRGHFGKVLLAEYKNTNEMFAIKALKKGDIVARDEVDSLMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFVMEYAAGGDLMMHIHTDVFSEPRAVFYAACVVLGLQYLHEHKIVYRDLKLDNLLLDTEGFVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDWWGLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGASEKDAEDVKKHPFFRLIDWSALMDKKVKPPFIPTIRGREDVSNFDDEFTSEAPILTPPREPRILSEEEQEMFRDFDYIADWC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Xenopus
100
Dog
88
Horse
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16512/Q16513 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S3 Phosphorylation
S8 Phosphorylation
K53 Ubiquitination
T63 Phosphorylation
T64 Phosphorylation Q16512 (PKN1)
S69 Phosphorylation
S80 Phosphorylation
S112 Phosphorylation
S205 Phosphorylation
S240 Phosphorylation
K243 Ubiquitination
K256 Ubiquitination
K263 Ubiquitination
S301 Phosphorylation
T311 Phosphorylation
T315 Phosphorylation
T353 Phosphorylation
R357 Methylation
R366 Methylation
S372 Phosphorylation
S374 Phosphorylation
S376 Phosphorylation Q16512 (PKN1)
S379 Phosphorylation
S380 Phosphorylation
K445 Ubiquitination
K448 Acetylation
S495 Phosphorylation
K496 Ubiquitination
T527 Phosphorylation
T529 Phosphorylation
T531 Phosphorylation
S533 Phosphorylation
S537 Phosphorylation
S540 Phosphorylation
S549 Phosphorylation
T557 Phosphorylation
S559 Phosphorylation
S561 Phosphorylation
S562 Phosphorylation
K565 Ubiquitination
S573 Phosphorylation
S575 Phosphorylation
S576 Phosphorylation
K607 Ubiquitination
S608 Phosphorylation
T611 Phosphorylation
K644 Ubiquitination
K664 Ubiquitination
K742 Ubiquitination
K763 Ubiquitination
Y768 Phosphorylation
T772 Phosphorylation
S773 Phosphorylation O15530 (PDPK1)
T774 Phosphorylation Q16512 (PKN1) , O15530 (PDPK1)
T778 Phosphorylation
R847 Methylation
S902 Phosphorylation
T914 Phosphorylation
S916 Phosphorylation
T925 Phosphorylation
Site PTM Type Enzyme
S3 Phosphorylation
S21 Phosphorylation
S29 Phosphorylation
K44 Ubiquitination
K58 Acetylation
K77 Ubiquitination
S110 Phosphorylation
T121 Phosphorylation
T124 Phosphorylation
K143 Ubiquitination
S163 Phosphorylation
K171 Ubiquitination
K184 Ubiquitination
K241 Ubiquitination
K247 Ubiquitination
S255 Phosphorylation
S264 Phosphorylation
K267 Ubiquitination
K272 Acetylation
K272 Ubiquitination
Y273 Phosphorylation
S274 Phosphorylation
K284 Ubiquitination
S289 Phosphorylation
S302 Phosphorylation
T304 Phosphorylation
S306 Phosphorylation
S310 Phosphorylation
Y318 Phosphorylation
K323 Ubiquitination
S349 Phosphorylation
K350 Ubiquitination
S360 Phosphorylation
S362 Phosphorylation
S367 Phosphorylation
S370 Phosphorylation
K376 Methylation
S379 Phosphorylation
K385 Ubiquitination
S408 Phosphorylation
K410 Ubiquitination
S413 Phosphorylation
S416 Phosphorylation
S497 Phosphorylation
K498 Ubiquitination
K502 Ubiquitination
T503 Phosphorylation
T516 Phosphorylation
S531 Phosphorylation
T533 Phosphorylation
S535 Phosphorylation P06493 (CDK1)
S559 Phosphorylation
S561 Phosphorylation
S582 Phosphorylation
S583 Phosphorylation
S603 Phosphorylation
R613 Methylation
S615 Phosphorylation
Y624 Phosphorylation
K625 Ubiquitination
T628 Phosphorylation
S631 Phosphorylation
Y635 Phosphorylation
S636 Phosphorylation
S646 Phosphorylation
Y676 Phosphorylation
K677 Ubiquitination
K686 Acetylation
S701 Phosphorylation
T729 Phosphorylation
K784 Ubiquitination
T792 Phosphorylation
K805 Ubiquitination
Y810 Phosphorylation
T814 Phosphorylation
S815 Phosphorylation
T816 Phosphorylation O15530 (PDPK1) , Q15118 (PDK1)
T820 Phosphorylation
K930 Ubiquitination
S952 Phosphorylation
T958 Phosphorylation
S967 Phosphorylation

PTMs - Q16512/Q16513 As Enzyme

Substrate Site Source
O95863 (SNAI1) S246 Uniprot
P00533 (EGFR) T678 Uniprot
P04049 (RAF1) S338 Uniprot
P04049 (RAF1) S339 Uniprot
P10636-6 (MAPT) T147 Uniprot
P10636-2 (MAPT) S156 Uniprot
P10636-2 (MAPT) S200 Uniprot
P10636-8 (MAPT) S214 Uniprot
P10636-2 (MAPT) S231 Uniprot
P10636-8 (MAPT) S258 Uniprot
P10636-6 (MAPT) S262 Uniprot
P10636-2 (MAPT) S263 Uniprot
P10636-6 (MAPT) S294 Uniprot
P10636-8 (MAPT) S320 Uniprot
P10636-8 (MAPT) S352 Uniprot
P10636 (MAPT) S637 Uniprot
P18669 (PGAM1) S23 Uniprot
P18669 (PGAM1) S118 Uniprot
P29966 (MARCKS) S159 Uniprot
P29966 (MARCKS) S163 Uniprot
P29966 (MARCKS) S170 Uniprot
P30307 (CDC25C) S216 Uniprot
P30622 (CLIP1) S312 Uniprot
P36871 (PGM1) T467 Uniprot
Q12778 (FOXO1) S256 Uniprot
Q13077 (TRAF1) S146 Uniprot
Q16512-1 (PKN1) T64 Uniprot
Q16512-2 (PKN1) T70 Uniprot
Q16512-1 (PKN1) S372 Uniprot
Q16512-1 (PKN1) S374 Uniprot
Q16512 (PKN1) S376 Uniprot
Q16512-2 (PKN1) S378 Uniprot
Q16512-1 (PKN1) S380 Uniprot
Q16512-1 (PKN1) T531 Uniprot
Q16512-2 (PKN1) T537 Uniprot
Q16512-1 (PKN1) S576 Uniprot
Q16512-2 (PKN1) S582 Uniprot
Q16512-1 (PKN1) S608 Uniprot
Q16512-2 (PKN1) S614 Uniprot
Q16512 (PKN1) T774 Uniprot
Q92974 (ARHGEF2) S886 Uniprot
Q9UKV0 (HDAC9) S253 Uniprot
Q9UQL6 (HDAC5) T292 Uniprot
Substrate Site Source

Research Backgrounds

Function:

PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.

PTMs:

Autophosphorylated; preferably on serine. Phosphorylated during mitosis.

Activated by limited proteolysis with trypsin.

(Microbial infection) In case of infection, polyubiquitinated by the bacterial E3 ubiquitin-protein ligase SspH1, leading to its proteasomal degradation.

Subcellular Location:

Cytoplasm. Nucleus. Endosome. Cell membrane>Peripheral membrane protein. Cleavage furrow. Midbody.
Note: Associates with chromatin in a ligand-dependent manner. Localization to endosomes is mediated via its interaction with RHOB. Association to the cell membrane is dependent on Ser-377 phosphorylation. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells.

Subunit Structure:

Interacts with ZFAND6 (By similarity). Interacts with ANDR. Interacts with PRKCB. Interacts (via REM 1 and REM 2 repeats) with RAC1. Interacts (via REM 1 repeat) with RHOA. Interacts with RHOB. Interacts (via C-terminus) with PDPK1. Interacts with CCNT2; enhances MYOD1-dependent transcription. Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and ZAK.

(Microbial infection) Interacts (via the second REM repeat) with S.typhimurium E3 ubiquitin-protein ligase SspH1 (via the leucine-rich repeat region).

Family&Domains:

The C1 domain does not bind the diacylglycerol (DAG).

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Function:

PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Involved in the negative regulation of ciliogenesis.

(Microbial infection) Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication.

PTMs:

Autophosphorylated. Phosphorylated during mitosis. Phosphorylated by CDK10.

Activated by limited proteolysis with trypsin (By similarity). Proteolytically cleaved by caspase-3 during the induction of apoptotic cell death.

Subcellular Location:

Cytoplasm. Nucleus. Membrane. Cell projection>Lamellipodium. Cytoplasm>Cytoskeleton. Cleavage furrow. Midbody. Cell junction.
Note: Colocalizes with PTPN13 in lamellipodia-like structures, regions of large actin turnover. Accumulates during telophase at the cleavage furrow and concentrates finally around the midbody in cytokinesis. Recruited to nascent cell-cell contacts at the apical surface of cells. In the course of viral infection, colocalizes with HCV NS5B at perinuclear region in the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells.

Subunit Structure:

Interacts (via the REM repeats) with RHOA (GTP-bound form preferentially) and interacts (via the REM repeats) with RAC1 (GTP-bound form preferentially); the interactions induce its autophosphorylation. Interacts with RHOC. Interacts with NCK1 and NCK2. Interacts with NCK1 (via SH3 domains) (By similarity). Interacts with CD44. Interacts (via C-terminal kinase domain) with PDPK1; the interaction stimulates PDPK1 kinase activity. Interacts with MAP3K2; the interaction activates PRK2 kinase activity in a MAP3K2-independent kinase activity. Interacts (via C-terminal domain) with AKT1; the interaction occurs with the C-terminal cleavage product of PRK2 in apoptotic cells. Interacts (via C-terminus) with PTPN13 (via PDZ 3 domain). Interacts with CDK10.

(Microbial infection) Interacts with HCV NS5B (via N-terminal finger domain).

Family&Domains:

The N-terminal regioninterferes with the interaction between AKT1 and the C-terminal regionof PKN2.

The C1 domain does not bind the diacylglycerol (DAG).

The apoptotic C-terminal cleavage product inhibits EGF-induced kinase activity of AKT1 phosphorylation at 'Thr-308' and 'Ser-473' sites, PDPK1 autophosphorylation and kinases PRKCD and PRKCZ phosphorylations.

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

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