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$450 200ul

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  • Product Name
    Phospho-SYN2 (Ser425) Antibody
  • Catalog No.
    AF8514
  • RRID
    AB_2840568
  • Source
    Rabbit
  • Application
    WB,ELISA(peptide)
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig, Bovine, Horse, Sheep, Rabbit, Dog
  • UniProt
  • Mol.Wt
    78 kD;
    63kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

SYN 2; SYN II; SYN IIa; SYN IIb; SYN2; Synapsin 2; Synapsin II isoform IIa; Synapsin II isoform IIb; Synapsin2; SynapsinII; SYNII; SYNIIa; SYNIIb;

Applications:

WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig, Bovine, Horse, Sheep, Rabbit, Dog

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

Phospho-SYN2 (Ser425) Antibody detects endogenous levels of SYN2 only when phosphorylated at Ser425.

RRID:

AB_2840568
Please cite this product as: Affinity Biosciences Cat# AF8514, RRID:AB_2840568.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human SYN2 around the phosphorylation site of Ser425.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

SYN2

Molecular Weight:

Observed Mol.Wt.: 78 kD.
Predicted Mol.Wt.: 63kDa(Calculated)..

Tissue Specificity:

Q92777 SYN2_HUMAN:
Central and peripheral nervous systems.

Sequence:
MMNFLRRRLSDSSFIANLPNGYMTDLQRPEPQQPPPPPPPGPGAASASAAPPTASPGPERRPPPASAPAPQPAPTPSVGSSFFSSLSQAVKQTAASAGLVDAPAPAPAAARKAKVLLVVDEPHADWAKCFRGKKVLGDYDIKVEQAEFSELNLVAHADGTYAVDMQVLRNGTKVVRSFRPDFVLIRQHAFGMAENEDFRHLIIGMQYAGLPSINSLESIYNFCDKPWVFAQLVAIYKTLGGEKFPLIEQTYYPNHKEMLTLPTFPVVVKIGHAHSGMGKVKVENHYDFQDIASVVALTQTYATAEPFIDSKYDIRVQKIGNNYKAYMRTSISGNWKTNTGSAMLEQIAMSDRYKLWVDTCSEMFGGLDICAVKAVHGKDGKDYIFEVMDCSMPLIGEHQVEDRQLITELVISKMNQLLSRTPALSPQRPLTTQQPQSGTLKDPDSSKTPPQRPPPQGGPGQPQGMQPPGKVLPPRRLPPGPSLPPSSSSSSSSSSSAPQRPGGPTTHGDAPSSSSSLAEAQPPLAAPPQKPQPHPQLNKSQSLTNAFSFSESSFFRSSANEDEAKAETIRSLRKSFASLFSD

Research Background

Function:

Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. May play a role in noradrenaline secretion by sympathetic neurons (By similarity).

Post-translational Modifications:

Phosphorylation at Ser-10 dissociates synapsins from synaptic vesicles. Phosphorylation at Ser-425 by MAPK1/ERK2 and/or MAPK3/ERK1 may play a role in noradrenaline secretion by sympathetic neurons (By similarity).

Subcellular Location:

Cell junction>Synapse.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Central and peripheral nervous systems.

Subunit Structure:

Interacts with CAPON.

Similarity:

The A region binds phospholipids with a preference for negatively charged species.

Belongs to the synapsin family.

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Catalog Number :

AF8514-BP
(Blocking peptide available as AF8514-BP)

Price/Size :

$350/1mg.
Tips: For modified antibodies, we provide modified peptides(0.5mg) and non-modified peptides(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Horse
90%
Bovine
90%
Sheep
90%
Dog
90%
Rabbit
90%
Chicken
70%
Xenopus
0%
Zebrafish
0%
High similarity Medium similarity Low similarity No similarity
Q92777 as Substrate
Site PTM Type Enzyme
S10 Phosphorylation
T421 Phosphorylation
S425 Phosphorylation
T432 Phosphorylation
K441 Ubiquitination
S488 Phosphorylation
S489 Phosphorylation
S490 Phosphorylation
S491 Phosphorylation
S542 Phosphorylation
S575 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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