TRMT11 Antibody | Affinity Biosciences

WB
IF/ICC

1/3

Western blot analysis of extracts from mouse heart, using TRMT11 Antibody.

Product:	TRMT11 Antibody
Catalog:	DF10060
Description:	Rabbit polyclonal antibody to TRMT11
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	53 kDa; 53kD(Calculated).
Uniprot:	Q7Z4G4
RRID:	AB_2840640

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(91%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(82%), Dog(91%)

Clonality:

Polyclonal

Specificity:

TRMT11 Antibody detects endogenous levels of total TRMT11.

RRID:

AB_2840640
Cite Format: Affinity Biosciences Cat# DF10060, RRID:AB_2840640.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

Uniprot:

Q7Z4G4(TRM11_HUMAN) >>Visit HPA database.

Gene(ID):

TRMT11(60487)

Sequence:

Q7Z4G4 TRM11_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALSCTLNRYLLLMAQEHLEFRLPEIKSLLLLFGGQFASSQETYGKSPFWILSIPSEDIARNLMKRTVCAKSIFELWGHGQSPEELYSSLKNYPVEKMVPFLHSDSTYKIKIHTFNKTLTQEEKIKRIDALEFLPFEGKVNLKKPQHVFSVLEDYGLDPNCIPENPHNIYFGRWIADGQRELIESYSVKKRHFIGNTSMDAGLSFIMANHGKVKENDIVFDPFVGTGGLLIACAHFGAYVYGTDIDYNTVHGLGKATRKNQKWRGPDENIRANLRQYGLEKYYLDVLVSDASKPSWRKGTYFDAIITDPPYGIRESTRRTGSQKEIPKGIEKWEKCPESHVPVSLSYHLSDMFLDLLNFAAETLVLGGRLVYWLPVYTPEYTEEMVPWHPCLELVSNCEQKLSSHTSRRLITMEKVKKFENRDQYSHLLSDHFLPYQGHNSFREKYFSGVTKRIAKEEKSTQE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Pig

Bovine

Sheep

Dog

Rabbit

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7Z4G4 As Substrate

Q7Z4G4

Site	PTM Type	Source
A2	Acetylation	Uniprot
Y44	Phosphorylation	Uniprot
K109	Ubiquitination	Uniprot
K111	Ubiquitination	Uniprot
K117	Ubiquitination	Uniprot
K124	Ubiquitination	Uniprot
K139	Ubiquitination	Uniprot
Y186	Phosphorylation	Uniprot
K189	Ubiquitination	Uniprot
Y311	Phosphorylation	Uniprot
K324	Ubiquitination	Uniprot
K332	Ubiquitination	Uniprot
S441	Phosphorylation	Uniprot
K445	Ubiquitination	Uniprot
Y446	Phosphorylation	Uniprot
S448	Phosphorylation	Uniprot
K452	Ubiquitination	Uniprot
T461	Phosphorylation	Uniprot

Research Backgrounds

Q7Z4G4_TRM11_HUMAN

Function:

Catalytic subunit of an S-adenosyl-L-methionine-dependent tRNA methyltransferase complex that mediates the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs.

Family&Domains:

Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM11 methyltransferase family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

TRMT11 Antibody - #DF10060