Product: DYR1B Antibody
Catalog: AF0607
Description: Rabbit polyclonal antibody to DYR1B
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Rabbit, Dog
Mol.Wt.: 70kDa; 69kD(Calculated).
Uniprot: Q9Y463
RRID: AB_2834359

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
DYR1B Antibody detects endogenous levels of total DYR1B.
RRID:
AB_2834359
Cite Format: Affinity Biosciences Cat# AF0607, RRID:AB_2834359.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AOMS3; Dual specificity tyrosine (Y) phosphorylation regulated kinase 1B; Dual specificity tyrosine phosphorylation regulated kinase 1B; Dual specificity tyrosine-phosphorylation-regulated kinase 1B; DYR 1B; DYR1A; DYR1B; DYR1B_HUMAN; DYRK 1B; Dyrk1b; Minibrain related kinase; Minibrain-related kinase; MIRK; Mirk protein kinase;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9Y463 DYR1B_HUMAN:

Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines.

Description:
DYRK1B is a member of the DYRK family of protein kinases. DYRK1B contains a bipartite nuclear localization signal and is found mainly in muscle and testis. The protein is proposed to be involved in the regulation of nuclear functions. Three isoforms of DYRK1B have been identified differing in the presence of two alternatively spliced exons within the catalytic domain.
Sequence:
MAVPPGHGPFSGFPGPQEHTQVLPDVRLLPRRLPLAFRDATSAPLRKLSVDLIKTYKHINEVYYAKKKRRAQQAPPQDSSNKKEKKVLNHGYDDDNHDYIVRSGERWLERYEIDSLIGKGSFGQVVKAYDHQTQELVAIKIIKNKKAFLNQAQIELRLLELMNQHDTEMKYYIVHLKRHFMFRNHLCLVFELLSYNLYDLLRNTHFRGVSLNLTRKLAQQLCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGTPYDLAIDMWSLGCILVEMHTGEPLFSGSNEVDQMNRIVEVLGIPPAAMLDQAPKARKYFERLPGGGWTLRRTKELRKDYQGPGTRRLQEVLGVQTGGPGGRRAGEPGHSPADYLRFQDLVLRMLEYEPAARISPLGALQHGFFRRTADEATNTGPAGSSASTSPAPLDTCPSSSTASSISSSGGSSGSSSDNRTYRYSNRYCGGPGPPITDCEMNSPQVPPSQPLRPWAGGDVPHKTHQAPASASSLPGTGAQLPPQPRYLGRPPSPTSPPPPELMDVSLVGGPADCSPPHPAPAPQHPAASALRTRMTGGRPPLPPPDDPATLGPHLGLRGVPQSTAASS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
100
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y463 As Substrate

Site PTM Type Enzyme
S49 Phosphorylation
K57 Acetylation
Y63 Phosphorylation
Y64 Phosphorylation
Y111 Phosphorylation
K119 Ubiquitination
K127 Ubiquitination
K256 Ubiquitination
Y271 Phosphorylation Q9Y463 (DYRK1B)
Y273 Phosphorylation Q9Y463 (DYRK1B)
S276 Phosphorylation
S421 Phosphorylation Q9Y463 (DYRK1B) , P28482 (MAPK1)
Y485 Phosphorylation
S486 Phosphorylation
S576 Phosphorylation

PTMs - Q9Y463 As Enzyme

Substrate Site Source
O75533 (SF3B1) T434 Uniprot
P20823 (HNF1A) S249 Uniprot
P24385 (CCND1) T286 Uniprot
P24385 (CCND1) T288 Uniprot
P38936 (CDKN1A) S153 Uniprot
P46527 (CDKN1B) S10 Uniprot
Q02363 (ID2) T27 Uniprot
Q99801 (NKX3-1) S185 Uniprot
Q9UQL6 (HDAC5) S279 Uniprot
Q9Y463 (DYRK1B) Y271 Uniprot
Q9Y463 (DYRK1B) Y273 Uniprot
Q9Y463 (DYRK1B) S421 Uniprot

Research Backgrounds

Function:

Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. Enhances the transcriptional activity of TCF1/HNF1A and FOXO1. Inhibits epithelial cell migration. Mediates colon carcinoma cell survival in mitogen-poor environments. Inhibits the SHH and WNT1 pathways, thereby enhancing adipogenesis. In addition, promotes expression of the gluconeogenic enzyme glucose-6-phosphatase (G6PC).

PTMs:

Autophosphorylated on tyrosine residues. Phosphorylated by MAP kinase. Tyrosine phosphorylation may be required for dimerization.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines.

Subunit Structure:

Dimer. Interacts with DCOHM, MAP2K3/MKK3, RANBP9 and TCF1/HNF1A. Part of a complex consisting of RANBP9, RAN, DYRK1B and COPS5. Interacts with DCAF7.

Family&Domains:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.

Restrictive clause

 

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