Product: COX11 Antibody
Catalog: AF0601
Description: Rabbit polyclonal antibody to COX11
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Rabbit, Dog
Mol.Wt.: 31kDa; 31kD(Calculated).
Uniprot: Q9Y6N1
RRID: AB_2834373

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(80%), Rabbit(80%), Dog(80%)
Clonality:
Polyclonal
Specificity:
COX11 Antibody detects endogenous levels of total COX11.
RRID:
AB_2834373
Cite Format: Affinity Biosciences Cat# AF0601, RRID:AB_2834373.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

COX11; COX11 homolog; COX11 homolog, cytochrome c oxidase assembly protein (yeast); COX11_HUMAN; COX11P; Cytochrome c oxidase assembly protein COX11; Cytochrome c oxidase assembly protein COX11, mitochondrial; Cytochrome c oxidase subunit 11; mitochondrial;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9Y6N1 COX11_HUMAN:

Ubiquitous.

Description:
Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be a heme A biosynthetic enzyme involved in COX formation, according to the yeast mutant studies. However, the studies in Rhodobacter sphaeroides suggest that this gene is not required for heme A biosynthesis, but required for stable formation of the Cu(B) and magnesium centers of COX. This human protein is predicted to contain a transmembrane domain localized in the mitochondrial inner membrane. A pseudogene COX11P has been found on chromosome 6.
Sequence:
MGGLWRPGWRCVPFCGWRWIHPGSPTRAAERVEPFLRPEWSGTGGAERGLRWLGTWKRCSLRARHPALQPPRRPKSSNPFTRAQEEERRRQNKTTLTYVAAVAVGMLGASYAAVPLYRLYCQTTGLGGSAVAGHASDKIENMVPVKDRIIKISFNADVHASLQWNFRPQQTEIYVVPGETALAFYRAKNPTDKPVIGISTYNIVPFEAGQYFNKIQCFCFEEQRLNPQEEVDMPVFFYIDPEFAEDPRMIKVDLITLSYTFFEAKEGHKLPVPGYN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
80
Dog
80
Rabbit
80
Pig
70
Bovine
70
Sheep
70
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6N1 As Substrate

Site PTM Type Enzyme
S24 Phosphorylation
K57 Ubiquitination
Y117 Phosphorylation
K138 Ubiquitination
K146 Ubiquitination
K188 Ubiquitination
K193 Ubiquitination
K265 Ubiquitination
K269 Ubiquitination

Research Backgrounds

Function:

Exerts its effect at some terminal stage of cytochrome c oxidase synthesis, probably by being involved in the insertion of the copper B into subunit I.

Subcellular Location:

Mitochondrion inner membrane>Single-pass membrane protein>Intermembrane side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with CNNM4/ACDP4.

Family&Domains:

Belongs to the COX11/CtaG family.

Research Fields

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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