Product: Gli1 Antibody
Catalog: DF7523
Source: Rabbit
Application: WB, IHC, IF/ICC, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Mol.Wt.: 118 kD; 118kD(Calculated).
Uniprot: P08151
RRID: AB_2841022

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Gli1 Antibody detects endogenous levels of total Gli1.
RRID:
AB_2841022
Cite Format: Affinity Biosciences Cat# DF7523, RRID:AB_2841022.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Gli 1; GLI; GLI family zinc finger 1; GLI Kruppel family member 1; gli1; GLI1_HUMAN; Glioma associated oncogene 1; Glioma associated oncogene homolog 1 (zinc finger protein); Glioma associated oncogene homolog; Glioma-associated oncogene; Oncogene GLI; Zfp 5; Zfp5; Zinc finger protein GLI 1; Zinc finger protein GLI1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P08151 GLI1_HUMAN:

Detected in testis (at protein level) (PubMed:2105456). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract (PubMed:19878745). Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues (PubMed:19706761).

Sequence:
MFNSMTPPPISSYGEPCCLRPLPSQGAPSVGTEGLSGPPFCHQANLMSGPHSYGPARETNSCTEGPLFSSPRSAVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPAQMNHQKGPSPSFGVQPCGPHDSARGGMIPHPQSRGPFPTCQLKSELDMLVGKCREEPLEGDMSSPNSTGIQDPLLGMLDGREDLEREEKREPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEHEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAPSISTVEPKREREGGPIREESRLTVPEGAMKPQPSPGAQSSCSSDHSPAGSAANTDSGVEMTGNAGGSTEDLSSLDEGPCIAGTGLSTLRRLENLRLDQLHQLRPIGTRGLKLPSLSHTGTTVSRRVGPPVSLERRSSSSSSISSAYTVSRRSSLASPFPPGSPPENGASSLPGLMPAQHYLLRARYASARGGGTSPTAASSLDRIGGLPMPPWRSRAEYPGYNPNAGVTRRASDPAQAADRPAPARVQRFKSLGCVHTPPTVAGGGQNFDPYLPTSVYSPQPPSITENAAMDARGLQEEPEVGTSMVGSGLNPYMDFPPTDTLGYGGPEGAAAEPYGARGPGSLPLGPGPPTNYGPNPCPQQASYPDPTQETWGEFPSHSGLYPGPKALGGTYSQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSEGPPHPQPLFSHYPQPSPPQYLQSGPYTQPPPDYLPSEPRPCLDFDSPTHSTGQLKAQLVCNYVQSQQELLWEGGGREDAPAQEPSYQSPKFLGGSQVSPSRAKAPVNTYGPGFGPNLPNHKSGSYPTPSPCHENFVVGANRASHRAAAPPRLLPPLPTCYGPLKVGGTNPSCGHPEVGRLGGGPALYPPPEGQVCNPLDSLDLDNTQLDFVAILDEPQGLSPPPSHDQRGSSGHTPPPSGPPNMAVGNMSVLLRSLPGETEFLNSSA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08151 As Substrate

Site PTM Type Enzyme
Phosphorylation
Ubiquitination
T63 Phosphorylation
S69 Phosphorylation
S84 Phosphorylation P23443 (RPS6KB1)
S102 Phosphorylation P54646 (PRKAA2)
S130 Phosphorylation
R161 Methylation
S201 Phosphorylation
S204 Phosphorylation
T205 Phosphorylation
S243 Phosphorylation P41743 (PRKCI)
T304 Phosphorylation P41743 (PRKCI)
K319 Ubiquitination
K365 Acetylation
K371 Acetylation
T374 Phosphorylation P17612 (PRKACA)
S408 Phosphorylation P54646 (PRKAA2)
K518 Acetylation
S521 Phosphorylation
T527 Phosphorylation
S538 Phosphorylation
S544 Phosphorylation P17612 (PRKACA)
S545 Phosphorylation
S560 Phosphorylation P17612 (PRKACA)
R597 Methylation
S602 Phosphorylation
S622 Phosphorylation
S640 Phosphorylation P17612 (PRKACA)
S934 Phosphorylation
S937 Phosphorylation
S939 Phosphorylation
S968 Phosphorylation
S1071 Phosphorylation
T1074 Phosphorylation P54646 (PRKAA2)
S1078 Phosphorylation

Research Backgrounds

Function:

Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling.

Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration.

PTMs:

Phosphorylated in vitro by ULK3.

Acetylation at Lys-518 down-regulates transcriptional activity. Deacetylated by HDAC1.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Tethered in the cytoplasm by binding to SUFU (PubMed:10806483). Activation and translocation to the nucleus is promoted by interaction with STK36 (PubMed:10806483). Phosphorylation by ULK3 may promote nuclear localization (PubMed:19878745). Translocation to the nucleus is promoted by interaction with ZIC1 (PubMed:11238441).

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in testis (at protein level). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues.

Subunit Structure:

Interacts with KIF7 (By similarity). Interacts with STK36. Interacts with ZIC1; the interaction enhances transcription activation. Interacts with SUFU; this inhibits transcriptional activation by GLI1.

Family&Domains:

Belongs to the GLI C2H2-type zinc-finger protein family.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

References

1). Jiang Y et al. Hedgehog pathway inhibition causes primary follicle atresia and decreases female germline stem cell proliferation capacity or stemness. Stem Cell Res Ther 2019 Jul 5;10(1):198 (PubMed: 31277696) [IF=5.116]

Application: IHC    Species: mouse    Sample: ovarian

Fig. 1 | Hh signaling pathway activity decreases with ovarian aging. a mRNA expression levels of Gli1, Ptch1, and Cyclin D1 in three mice of reproductive age. b, c Protein expression levels.d IHC detection of key members of the Hh pathway, Gli1, Ptch1, and Cyclin D1, in the OSE. Bar is 10 μm. *p < 0.05 and **p < 0.01

Application: IF/ICC    Species: mouse    Sample: ovarian

Fig. 2| Ovarian aging has a similar trend as the decrease in Hh activity. a mRNA levels of Mvh decrease gradually along with ovarian aging. bOct4 mRNA levels have the same trend as Mvh levels. c, d Protein levels of Mvh and Oct4 at three reproductive age points. e, f Dual-IF showed the coexpression of Mvh and Gli1, Oct4, and Gli1 in OSE. Bar is 20 μm. *p < 0.05 and **p < 0.01

Application: WB    Species: mouse    Sample: ovarian

Fig. 3| Pathological ovaries exhibited a decline in Hh pathway activity. a HE staining of a normal ovary and POF ovary. Bar is 200 μm. b The ratio of atretic follicles of normal and POF ovaries. c IHC shows that the Mvh-positive follicles and FGSCs dramatically disappeared in the POF model;green arrows are FGSCs, and black arrow represents primordial follicles, the scale is 100 μm. d–f mRNA and protein expression levels of Mvh, Oct4, Gli1,Ptch1, and Cyclin D1. *p < 0.05 and **p < 0.01

Application: WB    Species: mouse    Sample: ovaries

Fig. |1 Hh signaling pathway activity decreases with ovarian aging. a mRNA expression levels of Gli1, Ptch1, and Cyclin D1 in three mice of reproductive age. b, c Protein expression levels.

Application: IHC    Species: mouse    Sample: ovaries

Fig. |1 Hh signaling pathway activity decreases with ovarian aging. a mRNA expression levels of Gli1, Ptch1, and Cyclin D1 in three mice of reproductive age. b, c Protein expression levels.d IHC detection of key members of the Hh pathway, Gli1, Ptch1, and Cyclin D1, in the OSE. Bar is 10 μm. *p < 0.05 and **p < 0.01

Application: IF/ICC    Species: mouse    Sample: ovaries

Fig. 2 |Ovarian aging has a similar trend as the decrease in Hh activity. a mRNA levels of Mvh decrease gradually along with ovarian aging. bOct4 mRNA levels have the same trend as Mvh levels. c, d Protein levels of Mvh and Oct4 at three reproductive age points. e, f Dual-IF showed the coexpression of Mvh and Gli1, Oct4, and Gli1 in OSE. Bar is 20 μm. *p < 0.05 and **p < 0.01

2). Jiang Y et al. Resveratrol Plays a Protective Role against Premature Ovarian Failure and Prompts Female Germline Stem Cell Survival. Int J Mol Sci 2019 Jul 23;20(14) (PubMed: 31340581) [IF=4.556]

3). Wang C et al. Gankyrin activates the hedgehog signalling to drive metastasis in osteosarcoma. J Cell Mol Med 2021 Jun 5. (PubMed: 34089292) [IF=4.486]

4). Qiu P et al. Inoscavin A, a pyrone compound isolated from a Sanghuangporus vaninii extract, inhibits colon cancer cell growth and induces cell apoptosis via the hedgehog signaling pathway. Phytomedicine 2022 Feb;96:153852. (PubMed: 35026508) [IF=4.268]

5). Zhao J et al. Dehydroepiandrosterone alleviates E. Coli O157:H7-induced inflammation by preventing the activation of p38 MAPK and NF-κB pathways in mice peritoneal macrophages. Mol Immunol 2019 Jul 24;114:114-122 (PubMed: 31351412) [IF=3.641]

6). Qu JY et al. Hedgehog Signaling Pathway Regulates the Proliferation and Differentiation of Rat Meibomian Gland Epithelial Cells. Invest Ophthalmol Vis Sci 2021 Feb 1;62(2):33. (PubMed: 33616621) [IF=3.470]

7). Sun X et al. (‑)‑Epigallocatechin‑3‑gallate inhibits bladder cancer stem cells via suppression of sonic hedgehog pathway. Oncol Rep 2019 Jul;42(1):425-435 (PubMed: 31180522) [IF=3.417]

Application: WB    Species: human    Sample: Bladder CSCs

Figure 3.| - EGCG induces apoptosis and reduces the expression of cell proliferation-related proteins on bladder CSCs. Bladder CSCs were treated with different concentrations of EGCG for 7 days. (D) The expression levels of Shh signaling pathway-related proteins including Shh, Smo, Gli1 and Gli2 were detected by western blot analysis. Data are expressed as mean ± standard deviation. *P<0.05, **P<0.01 vs. the EGCG-0 µm group. EGCG, (−)-epigallocatechin-3-gallate; CSCs, cancer stem cells; PCNA, proliferating cell nuclear antigen; Shh, sonic hedgehog protein; Smo, smoothened homolog; Gli, zinc finger protein GLI.

8). Fan J et al. Curcumin mitigates the epithelial-to-mesenchymal transition in biliary epithelial cells through upregulating CD109 expression. Drug Dev Res 2019 Aug 12 (PubMed: 31403228)

Application: WB    Species: human    Sample: HIBECs

FIGURE 5 |Curcumin suppresses the TGF-β1-activated Hedgehog signaling in HIBECs. (a) Quantitative analysis of Shh, Gli1, Smo, Ptch-1, and Sufu protein expression.

9). Li Y et al. Shen-Shuai-Ling Formulation Attenuates Renal Interstitial Fibrosis in Chronic Kidney Disease by Regulating SHH-Gli1 Signaling Pathway. Evid Based Complement Alternat Med 2022 Feb 12;2022:3754985. (PubMed: 35190746)

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