Acetyl-Lysine Antibody - #DF7729
(3)
(3)
| Product: | Acetyl-Lysine Antibody |
| Catalog: | DF7729 |
| Description: | Rabbit polyclonal antibody to Acetyl-Lysine |
| Application: | WB |
| Reactivity: | Human, All |
| Uniprot: | |
| RRID: | AB_2841197 |
Product Info
Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,All
Clonality:
Polyclonal
Specificity:
Acetyl-Lysine Antibody detects endogenous levels of Acetyl-Lysine Antibody only when acetylated at Lysine Antibody.
RRID:
AB_2841197
Cite Format: Affinity Biosciences Cat# DF7729, RRID:AB_2841197.
Cite Format: Affinity Biosciences Cat# DF7729, RRID:AB_2841197.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Immunogens
Immunogen:
A synthesized peptide derived from human Lysine Antibody around the acetylation site of Lysine Antibody.
References
1). Resveratrol ameliorates triglyceride accumulation through FXR deacetylation in high glucose-treated HepG2 cells. Journal of Functional Foods
[IF=5.6]
Application: WB Species: Human Sample: HepG2 cells
Fig. 4. Effect of resveratrol on the acetylation of FXR in HepG2 cells. (A) The interaction of SIRT1 with FXR. (B) The relative protein levels of Ac-FXR in different groups of HepG2 cells detected by IP.
2). GRIM-19 inhibits proliferation and induces apoptosis in a p53-dependent manner in colorectal cancer cells through the SIRT7/PCAF/MDM2 axis. Experimental Cell Research
(PubMed: 34461110)
[IF=3.7]
Application: WB Species: Human Sample: CRC cells
Fig. 1. GRIM-19 expression is related to CRC progression. (A) Expression of GRIM-19 transcript in CRC tissues versus adjacent normal tissues. The plotted data
are from the GEPIA database. (B and C) Overall survival and disease-free survival among CRC patients with different GRIM-19 levels. The plotted data are from the
GEPIA database. (D and E) The expression of GRIM-19 and p53 in primary normal colorectal (N) and CRC (T) tissues as analyzed by Western blot assays. (F and G)
The expression of GRIM-19 in normal colorectal and CRC tissues was detected by IHC. Bar = 50 μ m. (H) The expression of GRIM-19 in CRC cell lines DLD-1, SW620,
HT15, HCT116 and HCT-8 as analyzed by Western blot assays.
3). Curcumol alleviates liver fibrosis by inducing endoplasmic reticulum stress-mediated necroptosis of hepatic stellate cells through Sirt1/NICD pathway. PeerJ
(PubMed: 35582617)
[IF=2.7]
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
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