SOD3 Antibody - #DF7753

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Product: SOD3 Antibody
Catalog: DF7753
Description: Rabbit polyclonal antibody to SOD3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 26 kDa; 26kD(Calculated).
Uniprot: P08294
RRID: AB_2841219

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
SOD3 Antibody detects endogenous levels of total SOD3.
RRID:
AB_2841219
Cite Format: Affinity Biosciences Cat# DF7753, RRID:AB_2841219.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC SOD; EC-SOD; Extracellular superoxide dismutase [Cu Zn]; Extracellular superoxide dismutase [Cu-Zn]; Extracellular superoxide dismutase; Extracellular superoxide dismutase precursor; MGC20077; SOD 3; SOD3; SODE_HUMAN; Superoxide dismutase 3 extracellular;

Immunogens

Immunogen:
Uniprot:

P08294(SODE_HUMAN) >>Visit HPA database.

Gene(ID):
SOD3(6649)
Expression:
P08294 SODE_HUMAN:

Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.

Sequence:
MLALLCSCLLLAAGASDAWTGEDSAEPNSDSAEWIRDMYAKVTEIWQEVMQRRDDDGALHAACQVQPSATLDAAQPRVTGVVLFRQLAPRAKLDAFFALEGFPTEPNSSSRAIHVHQFGDLSQGCESTGPHYNPLAVPHPQHPGDFGNFAVRDGSLWRYRAGLAASLAGPHSIVGRAVVVHAGEDDLGRGGNQASVENGNAGRRLACCVVGVCGPGLWERQAREHSERKKRRRESECKAA

PTMs - P08294 As Substrate

Site PTM Type Enzyme
N107 N-Glycosylation
Y159 Phosphorylation
S166 Phosphorylation
S172 Phosphorylation
S195 Phosphorylation

Research Backgrounds

Function:

Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.

Subcellular Location:

Secreted>Extracellular space.
Note: 99% of EC-SOD is anchored to heparan sulfate proteoglycans in the tissue interstitium, and 1% is located in the vasculature in equilibrium between the plasma and the endothelium.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in blood vessels, heart, lung, kidney and placenta. Major SOD isoenzyme in extracellular fluids such as plasma, lymph and synovial fluid.

Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the Cu-Zn superoxide dismutase family.

References

1). LncRNA MEG3 promotes osteogenesis of hBMSCs by regulating miR-21-5p/SOD3 axis. ACTA BIOCHIMICA POLONICA (PubMed: 35231166) [IF=1.7]

Application: IF/ICC    Species: Human    Sample:

Figure 6. Expression of SOD3 and COL2A1 in human bone mar-row-derived mesenchymal stem cells overexpressing long non-coding RNA (LncRNA) MEG3 after cultured in osteogenic medi-um for 14 days.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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