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$350 200ul

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  • Product Name
    Phospho-PKM2 (Tyr105) Antibody
  • Catalog No.
    DF7771
  • RRID
    AB_2841237
  • Source
    Rabbit
  • Application
    WB,ELISA(peptide)
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
  • UniProt
  • Mol.Wt
    58 kD;
    58kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

CTHBP; Cytosolic thyroid hormone binding protein; Cytosolic thyroid hormone-binding protein; KPYM_HUMAN; MGC3932; OIP 3; OIP-3; OIP3; OPA interacting protein 3; Opa-interacting protein 3; p58; PK muscle type; PK, muscle type; PK2; PK3; PKM; PKM2; pykm; Pyruvate kinase 2/3; Pyruvate kinase 3; Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle; Pyruvate kinase muscle isozyme; pyruvate kinase PKM; Pyruvate kinase, muscle 2; TCB; THBP1; Thyroid hormone binding protein 1; Thyroid hormone binding protein cytosolic; Thyroid hormone-binding protein 1; Tumor M2 PK; Tumor M2-PK;

Applications:

WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

Phospho-PKM2 (Tyr105) Antibody detects endogenous levels of PKM2 only when phosphorylated at Tyr105.

RRID:

AB_2841237
Please cite this product as: Affinity Biosciences Cat# DF7771, RRID:AB_2841237.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human PKM2 around the phosphorylation site of Tyr105.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

PKM

Molecular Weight:

Observed Mol.Wt.: 58 kD.
Predicted Mol.Wt.: 58kDa(Calculated)..

Subcellular Location:

Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.

Tissue Specificity:

P14618 KPYM_HUMAN:
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.

Sequence:
MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

Research Background

Function:

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. Promotes in a STAT1-dependent manner, the expression of the immune checkpoint protein CD274 in ARNTL/BMAL1-deficient macrophages (By similarity).

Post-translational Modifications:

ISGylated.

Under hypoxia, hydroxylated by EGLN3.

Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.

FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.

Subunit Structure:

Monomer and homotetramer. Exists as a monomer in the absence of D-fructose 1,6-bisphosphate (FBP), and reversibly associates to form a homotetramer in the presence of FBP. The monomeric form binds T3. Tetramer formation induces pyruvate kinase activity. The tetrameric form has high affinity for the substrate and is associated within the glycolytic enzyme complex. Exists in a nearly inactive dimeric form in tumor cells and the dimeric form has less affinity for the substrate. Binding to certain oncoproteins such as HPV-16 E7 oncoprotein can trigger dimerization. FBP stimulates the formation of tetramers from dimers. Interacts with HERC1, POU5F1 and PML. Interacts (isoform M2) with EGLN3; the interaction hydroxylates PKM under hypoxia and enhances binding to HIF1A. Interacts (isoform M2) with HIF1A; the interaction is enhanced by binding of EGLN3, promoting enhanced transcription activity under hypoxia. Interacts (isoform M2, but not isoform M1) with TRIM35; this interaction prevents FGFR1-dependent tyrosine phosphorylation. Interacts with JMJD8.

Similarity:

Belongs to the pyruvate kinase family.

Research Fields

Research Fields:

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.(View pathway)
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
· Organismal Systems > Endocrine system > Glucagon signaling pathway.

WB Images(1)


ELISA analysis of DF7771 showing specificity to Phospho-PKM2 (Tyr105) Blocking Peptide peptide. Peptides concentration: 1ug/ml.
P-peptide: phospho-peptide; N-peptide: non-phospho-peptide.

Reference Citations:

1). Xu Q et al. Inhibition of PTP1B blocks pancreatic cancer progression by targeting the PKM2/AMPK/mTOC1 pathway. Cell Death Dis 2019 Nov 19;10(12):874 (PubMed: 31745071) [IF=6.304]

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Catalog Number :

DF7771-BP
(Blocking peptide available as DF7771-BP)

Price/Size :

$350/1mg.
Tips: For modified antibodies, we provide modified peptides(0.5mg) and non-modified peptides(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Bovine
100%
Sheep
100%
Dog
100%
Xenopus
100%
Rabbit
100%
Chicken
88%
Horse
0%
Zebrafish
0%
High similarity Medium similarity Low similarity No similarity
P14618 as Substrate
Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K3 Acetylation
K3 Methylation
K3 Ubiquitination
S6 Phosphorylation
T25 Phosphorylation
S37 Phosphorylation P28482 (MAPK1)
T41 Phosphorylation
T45 Phosphorylation Q96GD4 (AURKB)
C49 S-Nitrosylation
T50 Phosphorylation
S55 Phosphorylation
S57 Phosphorylation
T60 Phosphorylation
K62 Acetylation
K62 Ubiquitination
K66 Acetylation
K66 Ubiquitination
S77 Phosphorylation
T80 Phosphorylation
Y83 Phosphorylation
T87 Phosphorylation
K89 Acetylation
K89 Ubiquitination
R92 Methylation
T93 Phosphorylation
T95 Phosphorylation
S97 Phosphorylation
S100 Phosphorylation
Y105 Phosphorylation Q9UM73 (ALK)
K115 Acetylation
K115 Ubiquitination
K125 Ubiquitination
S127 Phosphorylation
T129 Phosphorylation
K135 Acetylation
K135 Ubiquitination
K136 Ubiquitination
K141 Acetylation
K141 Ubiquitination
T143 Phosphorylation
Y148 Phosphorylation P00533 (EGFR)
K151 Acetylation
K151 Ubiquitination
C152 S-Nitrosylation
Y161 Phosphorylation
K162 Acetylation
K162 Ubiquitination
K166 Acetylation
K166 Sumoylation
K166 Ubiquitination
S172 Phosphorylation
Y175 Phosphorylation
S182 Phosphorylation
K186 Acetylation
K186 Ubiquitination
K188 Acetylation
K188 Ubiquitination
T195 Phosphorylation
S202 Phosphorylation
S205 Phosphorylation
K206 Acetylation
K206 Ubiquitination
K207 Acetylation
K207 Ubiquitination
S222 Phosphorylation
K224 Acetylation
K224 Sumoylation
K224 Ubiquitination
K230 Acetylation
K230 Ubiquitination
S243 Phosphorylation
K247 Ubiquitination
S249 Phosphorylation
R255 Methylation
K256 Ubiquitination
K261 Acetylation
K261 Ubiquitination
K266 Acetylation
K266 Ubiquitination
S269 Phosphorylation
K270 Sumoylation
K270 Ubiquitination
R278 Methylation
S287 Phosphorylation
K305 Acetylation
K305 Ubiquitination
K311 Ubiquitination
R319 Methylation
K322 Ubiquitination
C326 S-Nitrosylation
T328 Phosphorylation
K336 Ubiquitination
K337 Ubiquitination
S346 Phosphorylation
S362 Phosphorylation
T365 Phosphorylation
K367 Acetylation
K367 Ubiquitination
Y370 Phosphorylation
Y390 Phosphorylation
T405 O-Glycosylation
S406 O-Glycosylation
S406 Phosphorylation
T409 Phosphorylation
T412 Phosphorylation
S420 Phosphorylation
K422 Acetylation
C423 S-Nitrosylation
C424 S-Nitrosylation
S425 Phosphorylation
T432 Phosphorylation
K433 Acetylation
K433 Ubiquitination
S434 Phosphorylation
Y444 Phosphorylation
T454 Phosphorylation Q9P1W9 (PIM2)
Y466 Phosphorylation
R467 Methylation
C474 S-Nitrosylation
K475 Ubiquitination
K498 Acetylation
K498 Ubiquitination
K505 Ubiquitination
T513 Phosphorylation
S519 Phosphorylation
T524 Phosphorylation
P14618 as PTM Enzyme
Substrate Site Source
O00161 (SNAP23) S95 Uniprot
O43684 (BUB3) Y207 Uniprot
P40763 (STAT3) Y705 Uniprot
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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