Product: SOAT1 Antibody
Catalog: DF7778
Description: Rabbit polyclonal antibody to SOAT1
Application: WB IHC
Reactivity: Human, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 65 kDa; 65kD(Calculated).
Uniprot: P35610
RRID: AB_2841242

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(92%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(92%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
SOAT1 Antibody detects endogenous levels of total SOAT1.
RRID:
AB_2841242
Cite Format: Affinity Biosciences Cat# DF7778, RRID:AB_2841242.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACACT; ACAT; ACAT-1; ACAT1; Acyl coenzyme A cholesterol acyltransferase 1; Acyl-coenzyme A:cholesterol acyltransferase 1; Cholesterol acyltransferase 1; SOAT; SOAT1; SOAT1_HUMAN; STAT; Sterol O acyltransferase 1; Sterol O-acyltransferase 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MVGEEKMSLRNRLSKSRENPEEDEDQRNPAKESLETPSNGRIDIKQLIAKKIKLTAEAEELKPFFMKEVGSHFDDFVTNLIEKSASLDNGGCALTTFSVLEGEKNNHRAKDLRAPPEQGKIFIARRSLLDELLEVDHIRTIYHMFIALLILFILSTLVVDYIDEGRLVLEFSLLSYAFGKFPTVVWTWWIMFLSTFSVPYFLFQHWATGYSKSSHPLIRSLFHGFLFMIFQIGVLGFGPTYVVLAYTLPPASRFIIIFEQIRFVMKAHSFVRENVPRVLNSAKEKSSTVPIPTVNQYLYFLFAPTLIYRDSYPRNPTVRWGYVAMKFAQVFGCFFYVYYIFERLCAPLFRNIKQEPFSARVLVLCVFNSILPGVLILFLTFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYYRTWNVVVHDWLYYYAYKDFLWFFSKRFKSAAMLAVFAVSAVVHEYALAVCLSFFYPVLFVLFMFFGMAFNFIVNDSRKKPIWNVLMWTSLFLGNGVLLCFYSQEWYARQHCPLKNPTFLDYVRPRSWTCRYVF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
92
Zebrafish
92
Chicken
92
Rabbit
92
Xenopus
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35610 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S8 Phosphorylation
S14 Phosphorylation
S16 Phosphorylation
K31 Ubiquitination
S33 Phosphorylation
T36 Phosphorylation
S38 Phosphorylation
K45 Ubiquitination
K62 Ubiquitination
K67 Ubiquitination
S71 Phosphorylation
K83 Ubiquitination
K104 Ubiquitination
K120 Ubiquitination
K283 Ubiquitination
K353 Ubiquitination
K531 Ubiquitination

Research Backgrounds

Function:

Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

May form homo- or heterodimers. Interacts with UBIAD1.

Family&Domains:

Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Research Fields

· Metabolism > Lipid metabolism > Steroid biosynthesis.

· Organismal Systems > Digestive system > Cholesterol metabolism.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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