CD2 Antibody - Internal - DF7894
-
- Product Name
- CD2 Antibody
-
- Catalog No.
- DF7894
-
- RRID
- AB_2841315
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- Source
- Rabbit
-
- Application
- WB,ELISA
-
- Reactivity
- Human
-
- UniProt
-
- Mol.Wt
- 39kD;
39kDa(Calculated). -
- Concentration
- 1mg/ml
Related Products
Product Information
Alternative Names:Expand▼
CD 2; CD2; CD2 antigen (p50), sheep red blood cell receptor; CD2 antigen; CD2 molecule; CD2_HUMAN; Erythrocyte receptor; FLJ46032; LFA-2; LFA-3 receptor; LFA2; LFA3 receptor; Ly-37; Lymphocyte function antigen 2; lymphocyte-function antigen-2; OTTHUMP00000024366; Rosette receptor; Sheep erythrocyte receptor; SRBC; T cell surface antigen CD2; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5; T-lymphocyte surface CD2 antigen; T11;
Applications:
WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human
Source:
Rabbit
Clonality:
Polyclonal
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Specificity:
CD2 Antibody detects endogenous levels of total CD2.
RRID:
AB_2841315
Please cite this product as: Affinity Biosciences Cat# DF7894, RRID:AB_2841315.
Format:
Liquid
Concentration:
1mg/ml
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Immunogen Information
Immunogen:
A synthesized peptide derived from human CD2, corresponding to a region within the internal amino acids.
Uniprot:
>>Visit The Human Protein Atlas
Gene ID:
Gene Name:
CD2
Molecular Weight:
Observed Mol.Wt.: 39kD.
Predicted Mol.Wt.: 39kDa(Calculated)..
Subcellular Location:
Membrane.
Sequence:
- P06729 CD2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSFPCKFVASFLLIFNVSSKGAVSKEITNALETWGALGQDINLDIPSFQMSDDIDDIKWEKTSDKKKIAQFRKEKETFKEKDTYKLFKNGTLKIKHLKTDDQDIYKVSIYDTKGKNVLEKIFDLKIQERVSKPKISWTCINTTLTCEVMNGTDPELNLYQDGKHLKLSQRVITHKWTTSLSAKFKCTAGNKVSKESSVEPVSCPEKGLDIYLIIGICGGGSLLMVFVALLVFYITKRKKQRSRRNDEELETRAHRVATEERGRKPHQIPASTPQNPATSQHPPPPPGHRSQAPSHRPPPPGHRVQHQPQKRPPAPSGTQVHQQKGPPLPRPRVQPKPPHGAAENSLSPSSN
Research Background
Function:
CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Subcellular Location:
Cell membrane>Single-pass type I membrane protein.
Subunit Structure:
Interacts with CD48 (By similarity). Interacts with CD58 (LFA-3). Interacts with CD2AP (By similarity). Interacts with PSTPIP1.
Research Fields
Research Fields:
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).(View pathway)
· Organismal Systems > Immune system > Hematopoietic cell lineage.(View pathway)
Protocols & Troubleshooting
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Catalog Number :
DF7894-BP
(Blocking peptide available as DF7894-BP)
Price/Size :
$350/1mg.
Tips: For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).
Function :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.
Format and storage :
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions :
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
P06729 as Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N89 | N-Glycosylation | Uniprot | |
| T99 | Phosphorylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| N141 | N-Glycosylation | Uniprot | |
| N150 | N-Glycosylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| T173 | Phosphorylation | Uniprot | |
| T258 | Phosphorylation | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| S294 | Phosphorylation | Uniprot | |
| K310 | Ubiquitination | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot |
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