Mitofusin 2 Antibody | Affinity Biosciences

Product:	Mitofusin 2 Antibody
Catalog:	DF8106
Description:	Rabbit polyclonal antibody to Mitofusin 2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	86 kDa; 86kD(Calculated).
Uniprot:	O95140
RRID:	AB_2841449

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(87%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%), Xenopus(86%)

Clonality:

Polyclonal

Specificity:

Mitofusin 2 Antibody detects endogenous levels of total Mitofusin 2.

RRID:

AB_2841449
Cite Format: Affinity Biosciences Cat# DF8106, RRID:AB_2841449.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CMT2A; CMT2A2; CPRP 1; CPRP1; EC 3.6.5.-; Fzo; HSG; hyperplasia suppressor gene; Hypertension related protein 1; KIAA0214; MARF; MFN 2; Mfn2; MFN2_HUMAN; Mitochondrial assembly regulatory factor; Mitofusin-2; Mitofusin2; Transmembrane GTPase MFN2;

Immunogens

Immunogen:

Uniprot:

O95140(MFN2_HUMAN) >>Visit HPA database.

Gene(ID):

MFN2(9927)

Expression:

O95140 MFN2_HUMAN:

Ubiquitous; expressed at low level. Highly expressed in heart and kidney.

Sequence:

O95140 MFN2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSLLFSRCNSIVTVKKNKRHMAEVNASPLKHFVTAKKKINGIFEQLGAYIQESATFLEDTYRNAELDPVTTEEQVLDVKGYLSKVRGISEVLARRHMKVAFFGRTSNGKSTVINAMLWDKVLPSGIGHTTNCFLRVEGTDGHEAFLLTEGSEEKRSAKTVNQLAHALHQDKQLHAGSLVSVMWPNSKCPLLKDDLVLMDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKHFFHKVSERLSRPNIFILNNRWDASASEPEYMEEVRRQHMERCTSFLVDELGVVDRSQAGDRIFFVSAKEVLNARIQKAQGMPEGGGALAEGFQVRMFEFQNFERRFEECISQSAVKTKFEQHTVRAKQIAEAVRLIMDSLHMAAREQQVYCEEMREERQDRLKFIDKQLELLAQDYKLRIKQITEEVERQVSTAMAEEIRRLSVLVDDYQMDFHPSPVVLKVYKNELHRHIEEGLGRNMSDRCSTAITNSLQTMQQDMIDGLKPLLPVSVRSQIDMLVPRQCFSLNYDLNCDKLCADFQEDIEFHFSLGWTMLVNRFLGPKNSRRALMGYNDQVQRPIPLTPANPSMPPLPQGSLTQEEFMVSMVTGLASLTSRTSMGILVVGGVVWKAVGWRLIALSFGLYGLLYVYERLTWTTKAKERAFKRQFVEHASEKLQLVISYTGSNCSHQVQQELSGTFAHLCQQVDVTRENLEQEIAAMNKKIEVLDSLQSKAKLLRNKAGWLDSELNMFTHQYLQPSR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Zebrafish

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95140 As Substrate

O95140

Site	PTM Type	Enzyme	Source
	Ubiquitination		Uniprot
S10	Phosphorylation		Uniprot
S27	Phosphorylation	P45984 (MAPK9)	Uniprot
K30	Ubiquitination		Uniprot
K36	Acetylation		Uniprot
K36	Ubiquitination		Uniprot
K79	Ubiquitination		Uniprot
K84	Ubiquitination		Uniprot
K98	Ubiquitination		Uniprot
T111	Phosphorylation		Uniprot
K154	Ubiquitination		Uniprot
K158	Ubiquitination		Uniprot
K171	Ubiquitination		Uniprot
K192	Ubiquitination		Uniprot
K243	Acetylation		Uniprot
K243	Ubiquitination		Uniprot
K307	Ubiquitination		Uniprot
K316	Ubiquitination		Uniprot
K355	Acetylation		Uniprot
K355	Ubiquitination		Uniprot
K357	Ubiquitination		Uniprot
K366	Ubiquitination		Uniprot
K406	Ubiquitination		Uniprot
K416	Ubiquitination		Uniprot
K420	Ubiquitination		Uniprot
T423	Phosphorylation		Uniprot
S442	Phosphorylation		Uniprot
K460	Ubiquitination		Uniprot
K463	Ubiquitination		Uniprot
K560	Ubiquitination		Uniprot
K662	Ubiquitination		Uniprot
K719	Ubiquitination		Uniprot
K720	Ubiquitination		Uniprot
K730	Ubiquitination		Uniprot
K737	Ubiquitination		Uniprot
S756	Phosphorylation		Uniprot

Research Backgrounds

O95140_MFN2_HUMAN

Function:

Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (Probable). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (By similarity). Plays an important role in the regulation of vascular smooth muscle cell proliferation (By similarity). Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress (By similarity). Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions (By similarity).

PTMs:

Phosphorylated by PINK1.

Ubiquitinated by non-degradative ubiquitin by PRKN, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.

Subcellular Location:

Mitochondrion outer membrane>Multi-pass membrane protein.
Note: Colocalizes with BAX during apoptosis.

Tissue Specificity:

Ubiquitous; expressed at low level. Highly expressed in heart and kidney.

Subunit Structure:

Forms homomultimers and heteromultimers with MFN1. Oligomerization is essential for mitochondrion fusion (Probable). Interacts with VAT1 (By similarity). Interacts with STOML2; may form heterooligomers. Interacts (phosphorylated) with PRKN. Interacts with EIF2AK3 (By similarity).

Family&Domains:

A helix bundle is formed by helices from the N-terminal and the C-terminal part of the protein. The GTPase domain cannot be expressed by itself, without the helix bundle. Rearrangement of the helix bundle and/or of the coiled coil domains may bring membranes from adjacent mitochondria into close contact, and thereby play a role in mitochondrial fusion.

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

References

1). Ginsenoside CK improves skeletal muscle insulin resistance by activating DRP1/PINK1-mediated mitophagy. Food & Function (PubMed: 36562271) [IF=6.1]

2). FGFR2 Mutation p.Cys342Arg Enhances Mitochondrial Metabolism-Mediated Osteogenesis via FGF/FGFR-AMPK-Erk1/2 Axis in Crouzon Syndrome. Cells (PubMed: 36231091) [IF=6.0]

Application: WB Species: Human Sample:

Figure 7 Mitochondrial dynamics participate in osteogenesis mediated by FGFR/FGFR2-AMPK pathway: (A) Mito-tracker red and mitochondria 2D analysis showed more fragmented or punctate mitochondria with fewer and shorter branches in the MT group, while there were widely reticular mitochondria with longer branches in the WT group. (B) qRT-PCR and Western blot analysis demonstrated that the expressions of mitochondrial-fusion-related factors Mfn2 and Opa1 were downregulated, however, the expression of mitochondrial-fission-related factor Drp1 was upregulated in the MT group compared to the WT group. (C) Mito-tracker red and mitochondria 2D analysis showed a filamentous network of mitochondria with more and longer branches widely spread in the cytoplasm in the siFGFR2 group. (D) qPCR and Western blot analysis showed that the expression of fusion-related genes Mfn2 and Opa1 was increased and the expression of fission-related gene Drp1 was decreased in the siFGFR2 group. (E) After treatment with Compound C, the morphology of the mitochondria which should have tended to split adopted a fused reticular structure with more and longer branches, as shown by Mito-tracker red and mitochondria 2D analysis. (F) Western blot analysis showed the level of MFN2 and OPA1 was increased and then level of DRP1 was decreased, as induced by Compound C. p values were significant at * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.

3). Targeted inhibition of CX3CL1 limits podocytes ferroptosis to ameliorate cisplatin-induced acute kidney injury. Molecular medicine (Cambridge, Mass.) (PubMed: 37875838) [IF=5.7]

Application: WB Species: Mouse Sample: kidney tissues

Fig. 4 The mitochondrial function was improved by CX3CL1 deficiency in mice with cisplatin-induced AKI. A TEM images of renal tissues were captured. Scale bar = 500 nm. B The relative expression profiles of the mitochondrial proteins UCP2, Mfn2, and PGC1α in kidney tissues were determined by Western blotting. C DHE staining was conducted to determine the ROS level in renal tissues. Scale bar = 20 μm. D TEM was employed to capture images of podocytes. Scale bar = 500 nm. E Western blot analysis illustrating the relative expression patterns of the mitochondrial proteins UCP2, Mfn2, and PGC1α in podocytes. F The ROS level was detected through DCFH-DA labeling. Scale bar = 10 μm. G JC-1 staining was conducted to detect MMP in podocytes. Scale bar = 10 μm. (AKI, acute kidney injury; TEM, transmission electron microscopy; UCP2, uncoupling protein 2; Mfn2, Mitofusin 2; PGC1α, peroxisome proliferators-activated receptor γ coactivator l-alpha; DHE, dihydroethidium; ROS, reactive oxygen species; DCFH-DA, 2,7-dichlorodihydrofluorescein diacetate; MMP, matrix metalloproteinase; P value was calculated by one-way analysis of variance and Tukey’s test.

4). Effect and molecular mechanism of Sulforaphane alleviates brain damage caused by acute carbon monoxide poisoning:Network pharmacology analysis, molecular docking, and experimental evidence. Environmental toxicology (PubMed: 37860845) [IF=4.5]

5). Melatonin Alleviates Acute Kidney Injury by Inhibiting NRF2/Slc7a11 Axis-Mediated Ferroptosis. Oxidative Medicine and Cellular Longevity (PubMed: 35979396)

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Mitofusin 2 Antibody - #DF8106