RSF1 Antibody - #DF8109

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Product: RSF1 Antibody
Catalog: DF8109
Description: Rabbit polyclonal antibody to RSF1
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 164,250kD; 164kD(Calculated).
Uniprot: Q96T23
RRID: AB_2841452

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
RSF1 Antibody detects endogenous levels of total RSF1.
RRID:
AB_2841452
Cite Format: Affinity Biosciences Cat# DF8109, RRID:AB_2841452.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HBV pX associated protein 8; Hepatitis B virus x associated protein; HXBAP; p325; p325 subunit of RSF chromatin remodeling complex; remodeling and spacing factor 1; RSF 1; XAP8;

Immunogens

Immunogen:
Uniprot:

Q96T23(RSF1_HUMAN) >>Visit HPA database.

Gene(ID):
RSF1(51773)
Expression:
Q96T23 RSF1_HUMAN:

Ubiquitously expressed.

Sequence:
MATAAAAAAVMAPPGCPGSCPNFAVVCSFLERYGPLLDLPELPFPELERVLQAPPPDVGNGEVPKELVELHLKLMRKIGKSVTADRWEKYLIKICQEFNSTWAWEMEKKGYLEMSVECKLALLKYLCECQFDDNLKFKNIINEEDADTMRLQPIGRDKDGLMYWYQLDQDHNVRMYIEEQDDQDGSSWKCIVRNRNELAETLALLKAQIDPVLLKNSSQQDNSSRESPSLEDEETKKEEETPKQEEQKESEKMKSEEQPMDLENRSTANVLEETTVKKEKEDEKELVKLPVIVKLEKPLPENEEKKIIKEESDSFKENVKPIKVEVKECRADPKDTKSSMEKPVAQEPERIEFGGNIKSSHEITEKSTEETEKLKNDQQAKIPLKKREIKLSDDFDSPVKGPLCKSVTPTKEFLKDEIKQEEETCKRISTITALGHEGKQLVNGEVSDERVAPNFKTEPIETKFYETKEESYSPSKDRNIITEGNGTESLNSVITSMKTGELEKETAPLRKDADSSISVLEIHSQKAQIEEPDPPEMETSLDSSEMAKDLSSKTALSSTESCTMKGEEKSPKTKKDKRPPILECLEKLEKSKKTFLDKDAQRLSPIPEEVPKSTLESEKPGSPEAAETSPPSNIIDHCEKLASEKEVVECQSTSTVGGQSVKKVDLETLKEDSEFTKVEMDNLDNAQTSGIEEPSETKGSMQKSKFKYKLVPEEETTASENTEITSERQKEGIKLTIRISSRKKKPDSPPKVLEPENKQEKTEKEEEKTNVGRTLRRSPRISRPTAKVAEIRDQKADKKRGEGEDEVEEESTALQKTDKKEILKKSEKDTNSKVSKVKPKGKVRWTGSRTRGRWKYSSNDESEGSGSEKSSAASEEEEEKESEEAILADDDEPCKKCGLPNHPELILLCDSCDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCEKLEEQLQDLDVALKKKERAERRKERLVYVGISIENIIPPQEPDFSEDQEEKKKDSKKSKANLLERRSTRTRKCISYRFDEFDEAIDEAIEDDIKEADGGGVGRGKDISTITGHRGKDISTILDEERKENKRPQRAAAARRKKRRRLNDLDSDSNLDEEESEDEFKISDGSQDEFVVSDENPDESEEDPPSNDDSDTDFCSRRLRRHPSRPMRQSRRLRRKTPKKKYSDDDEEEESEENSRDSESDFSDDFSDDFVETRRRRSRRNQKRQINYKEDSESDGSQKSLRRGKEIRRVHKRRLSSSESEESYLSKNSEDDELAKESKRSVRKRGRSTDEYSEADEEEEEEEGKPSRKRLHRIETDEEESCDNAHGDANQPARDSQPRVLPSEQESTKKPYRIESDEEEDFENVGKVGSPLDYSLVDLPSTNGQSPGKAIENLIGKPTEKSQTPKDNSTASASLASNGTSGGQEAGAPEEEEDELLRVTDLVDYVCNSEQL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
100
Dog
100
Rabbit
100
Pig
90
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96T23 As Substrate

Site PTM Type Enzyme
K77 Acetylation
K80 Ubiquitination
K89 Ubiquitination
K136 Acetylation
K136 Ubiquitination
K138 Ubiquitination
K158 Ubiquitination
S186 Phosphorylation
K189 Ubiquitination
K215 Ubiquitination
S217 Phosphorylation
S218 Phosphorylation
S227 Phosphorylation
K252 Acetylation
K254 Sumoylation
K254 Ubiquitination
S255 Phosphorylation
S266 Phosphorylation
K277 Sumoylation
K278 Acetylation
K284 Sumoylation
K288 Ubiquitination
K294 Sumoylation
K297 Ubiquitination
K309 Sumoylation
S314 Phosphorylation
K316 Ubiquitination
K320 Ubiquitination
K323 Sumoylation
K337 Sumoylation
K358 Ubiquitination
K366 Ubiquitination
K381 Ubiquitination
K390 Sumoylation
K390 Ubiquitination
S392 Phosphorylation
S397 Phosphorylation
S406 Phosphorylation
T408 Phosphorylation
T410 Phosphorylation
K411 Ubiquitination
K415 Sumoylation
K419 Sumoylation
S429 Phosphorylation
S447 Phosphorylation
K456 Acetylation
K456 Sumoylation
K456 Ubiquitination
K463 Ubiquitination
K468 Sumoylation
K468 Ubiquitination
S471 Phosphorylation
S473 Phosphorylation
K498 Ubiquitination
T506 Phosphorylation
S516 Phosphorylation
S524 Phosphorylation
T539 Phosphorylation
S540 Phosphorylation
S544 Phosphorylation
S561 Phosphorylation
C562 S-Nitrosylation
S570 Phosphorylation
T594 Phosphorylation
S604 Phosphorylation
T614 Phosphorylation
S617 Phosphorylation
S622 Phosphorylation
T628 Phosphorylation
S629 Phosphorylation
S632 Phosphorylation
S643 Phosphorylation
S660 Phosphorylation
K663 Sumoylation
T668 Phosphorylation
K670 Sumoylation
K677 Sumoylation
S689 Phosphorylation
K698 Sumoylation
K698 Ubiquitination
K709 Ubiquitination
S748 Phosphorylation
K758 Acetylation
K758 Sumoylation
K758 Ubiquitination
K768 Sumoylation
S778 Phosphorylation
S782 Phosphorylation
K824 Ubiquitination
K825 Ubiquitination
S874 Phosphorylation
S882 Phosphorylation
K946 Ubiquitination
K959 Ubiquitination
Y973 Phosphorylation
S977 Phosphorylation
S990 Phosphorylation
K1004 Ubiquitination
K1039 Ubiquitination
K1050 Acetylation
K1050 Ubiquitination
T1056 Phosphorylation
K1061 Acetylation
S1096 Phosphorylation
S1098 Phosphorylation
S1105 Phosphorylation
S1135 Phosphorylation
S1139 Phosphorylation
T1141 Phosphorylation
Y1171 Phosphorylation
S1172 Phosphorylation
S1187 Phosphorylation
S1189 Phosphorylation
S1192 Phosphorylation
S1196 Phosphorylation
Y1217 Phosphorylation
S1221 Phosphorylation
S1223 Phosphorylation
S1226 Phosphorylation
S1229 Phosphorylation
K1234 Acetylation
S1245 Phosphorylation
S1246 Phosphorylation
S1247 Phosphorylation
S1249 Phosphorylation
S1252 Phosphorylation
Y1253 Phosphorylation
S1255 Phosphorylation
S1258 Phosphorylation
S1277 Phosphorylation
T1278 Phosphorylation
Y1281 Phosphorylation
S1282 Phosphorylation
T1305 Phosphorylation
S1310 Phosphorylation
S1325 Phosphorylation
S1336 Phosphorylation
T1337 Phosphorylation
K1339 Acetylation
Y1341 Phosphorylation
S1345 Phosphorylation
S1359 Phosphorylation P53350 (PLK1)
Y1363 Phosphorylation
S1364 Phosphorylation
S1370 Phosphorylation
T1371 Phosphorylation
S1375 Phosphorylation P06493 (CDK1)
K1378 Acetylation
K1386 Acetylation
T1388 Phosphorylation
K1390 Acetylation
S1391 Phosphorylation
T1393 Phosphorylation
S1406 Phosphorylation
S1438 Phosphorylation

Research Backgrounds

Function:

Required for assembly of regular nucleosome arrays by the RSF chromatin-remodeling complex. Facilitates transcription of hepatitis B virus (HBV) genes by the pX transcription activator. In case of infection by HBV, together with pX, it represses TNF-alpha induced NF-kappa-B transcription activation. Represses transcription when artificially recruited to chromatin by fusion to a heterogeneous DNA binding domain.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Interacts with SMARCA5/SNF2H to form the RSF complex. Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1. Also binds the HBV pX/HBx protein, which is required to activate transcription of the viral genome.

Restrictive clause

 

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