MYH11 Antibody - #DF8344

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Product: MYH11 Antibody
Catalog: DF8344
Description: Rabbit polyclonal antibody to MYH11
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Rabbit, Dog
Mol.Wt.: 227 kDa; 227kD(Calculated).
Uniprot: P35749
RRID: AB_2841611

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(92%), Horse(100%), Rabbit(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
MYH11 Antibody detects endogenous levels of total MYH11.
RRID:
AB_2841611
Cite Format: Affinity Biosciences Cat# DF8344, RRID:AB_2841611.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AAT4; DKFZp686D10126; DKFZp686D19237; FAA4; FLJ35232; MGC126726; MGC32963; MYH 11; MYH11; MYH11_HUMAN; Myosin 11; Myosin heavy chain 11; Myosin heavy chain 11 smooth muscle; Myosin heavy chain; Myosin heavy chain smooth muscle isoform; Myosin heavy polypeptide 11 smooth muscle; Myosin-11; SMHC; SMMHC; smooth muscle isoform; Smooth muscle myosin heavy chain 11 isoform SM2; Smooth muscle myosin heavy chain isoform SM2;

Immunogens

Immunogen:
Uniprot:

P35749(MYH11_HUMAN) >>Visit HPA database.

Gene(ID):
MYH11(4629)
Expression:
P35749 MYH11_HUMAN:

Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea. Isoform 1 is mostly found in slowly contracting tonic muscles.

Sequence:
MAQKGQLSDDEKFLFVDKNFINSPVAQADWAAKRLVWVPSEKQGFEAASIKEEKGDEVVVELVENGKKVTVGKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYFSGLIYTYSGLFCVVVNPYKHLPIYSEKIVDMYKGKKRHEMPPHIYAIADTAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDTSITGELEKQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAIRQARDERTFHIFYYMIAGAKEKMRSDLLLEGFNNYTFLSNGFVPIPAAQDDEMFQETVEAMAIMGFSEEEQLSILKVVSSVLQLGNIVFKKERNTDQASMPDNTAAQKVCHLMGINVTDFTRSILTPRIKVGRDVVQKAQTKEQADFAVEALAKATYERLFRWILTRVNKALDKTHRQGASFLGILDIAGFEIFEVNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGVLALLDEECWFPKATDKSFVEKLCTEQGSHPKFQKPKQLKDKTEFSIIHYAGKVDYNASAWLTKNMDPLNDNVTSLLNASSDKFVADLWKDVDRIVGLDQMAKMTESSLPSASKTKKGMFRTVGQLYKEQLGKLMTTLRNTTPNFVRCIIPNHEKRSGKLDAFLVLEQLRCNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACILMIKALELDPNLYRIGQSKIFFRTGVLAHLEEERDLKITDVIMAFQAMCRGYLARKAFAKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTKVKPLLQVTRQEEEMQAKEDELQKTKERQQKAENELKELEQKHSQLTEEKNLLQEQLQAETELYAEAEEMRVRLAAKKQELEEILHEMEARLEEEEDRGQQLQAERKKMAQQMLDLEEQLEEEEAARQKLQLEKVTAEAKIKKLEDEILVMDDQNNKLSKERKLLEERISDLTTNLAEEEEKAKNLTKLKNKHESMISELEVRLKKEEKSRQELEKLKRKLEGDASDFHEQIADLQAQIAELKMQLAKKEEELQAALARLDDEIAQKNNALKKIRELEGHISDLQEDLDSERAARNKAEKQKRDLGEELEALKTELEDTLDSTATQQELRAKREQEVTVLKKALDEETRSHEAQVQEMRQKHAQAVEELTEQLEQFKRAKANLDKNKQTLEKENADLAGELRVLGQAKQEVEHKKKKLEAQVQELQSKCSDGERARAELNDKVHKLQNEVESVTGMLNEAEGKAIKLAKDVASLSSQLQDTQELLQEETRQKLNVSTKLRQLEEERNSLQDQLDEEMEAKQNLERHISTLNIQLSDSKKKLQDFASTVEALEEGKKRFQKEIENLTQQYEEKAAAYDKLEKTKNRLQQELDDLVVDLDNQRQLVSNLEKKQRKFDQLLAEEKNISSKYADERDRAEAEAREKETKALSLARALEEALEAKEELERTNKMLKAEMEDLVSSKDDVGKNVHELEKSKRALETQMEEMKTQLEELEDELQATEDAKLRLEVNMQALKGQFERDLQARDEQNEEKRRQLQRQLHEYETELEDERKQRALAAAAKKKLEGDLKDLELQADSAIKGREEAIKQLRKLQAQMKDFQRELEDARASRDEIFATAKENEKKAKSLEADLMQLQEDLAAAERARKQADLEKEELAEELASSLSGRNALQDEKRRLEARIAQLEEELEEEQGNMEAMSDRVRKATQQAEQLSNELATERSTAQKNESARQQLERQNKELRSKLHEMEGAVKSKFKSTIAALEAKIAQLEEQVEQEAREKQAATKSLKQKDKKLKEILLQVEDERKMAEQYKEQAEKGNARVKQLKRQLEEAEEESQRINANRRKLQRELDEATESNEAMGREVNALKSKLRRGNETSFVPSRRSGGRRVIENADGSEEETDTRDADFNGTKASE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
92
Dog
92
Rabbit
92
Pig
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35749 As Substrate

Site PTM Type Enzyme
S8 Phosphorylation
S23 Phosphorylation
K67 Acetylation
K78 Acetylation
K83 Ubiquitination
S85 Phosphorylation
K129 Methylation
Y142 Phosphorylation
K143 Acetylation
K146 Acetylation
Y155 Phosphorylation
Y162 Phosphorylation
S173 Phosphorylation
K184 Ubiquitination
Y194 Phosphorylation
T208 Phosphorylation
K232 Ubiquitination
T233 Phosphorylation
K235 Ubiquitination
K244 Ubiquitination
K268 Ubiquitination
K362 Acetylation
K363 Acetylation
T367 Phosphorylation
S371 Phosphorylation
T376 Phosphorylation
S395 Phosphorylation
K547 Ubiquitination
S548 Phosphorylation
S559 Phosphorylation
T604 Phosphorylation
S637 Phosphorylation
S638 Phosphorylation
T652 Phosphorylation
K658 Methylation
T666 Phosphorylation
T667 Phosphorylation
R718 Methylation
Y761 Phosphorylation
S766 Phosphorylation
K767 Acetylation
K767 Ubiquitination
Y826 Phosphorylation
K828 Acetylation
K828 Ubiquitination
K842 Ubiquitination
S883 Phosphorylation
T900 Phosphorylation
Y903 Phosphorylation
K968 Ubiquitination
S1009 Phosphorylation
S1034 Phosphorylation
K1088 Acetylation
K1088 Ubiquitination
K1111 Acetylation
S1121 Phosphorylation
T1153 Phosphorylation
S1161 Phosphorylation
T1162 Phosphorylation
S1189 Phosphorylation
K1216 Acetylation
K1226 Ubiquitination
S1266 Phosphorylation
T1336 Phosphorylation
S1347 Phosphorylation
S1367 Phosphorylation
T1368 Phosphorylation
K1377 Acetylation
K1378 Acetylation
K1395 Acetylation
T1405 Phosphorylation
Y1408 Phosphorylation
Y1415 Phosphorylation
T1421 Phosphorylation
S1444 Phosphorylation
K1448 Acetylation
K1449 Acetylation
K1452 Acetylation
K1452 Methylation
K1452 Ubiquitination
S1465 Phosphorylation
Y1467 Phosphorylation
K1484 Acetylation
K1484 Ubiquitination
S1487 Phosphorylation
T1558 Phosphorylation
K1562 Ubiquitination
Y1601 Phosphorylation
T1603 Phosphorylation
K1619 Acetylation
K1649 Ubiquitination
S1667 Phosphorylation
T1674 Phosphorylation
K1680 Acetylation
K1681 Acetylation
R1701 Methylation
K1731 Acetylation
S1810 Phosphorylation
K1811 Acetylation
K1813 Acetylation
S1814 Phosphorylation
T1815 Phosphorylation
Y1868 Phosphorylation
K1925 Methylation
S1954 Phosphorylation
T1958 Phosphorylation
T1960 Phosphorylation

Research Backgrounds

Function:

Muscle contraction.

Subcellular Location:

Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea. Isoform 1 is mostly found in slowly contracting tonic muscles.

Subunit Structure:

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Family&Domains:

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction.   (View pathway)

References

1). C/EBPα-Mediated Transcriptional Activation of PIK3C2A Regulates Autophagy, Matrix Metalloproteinase Expression, and Phenotypic of Vascular Smooth Muscle Cells in Aortic Dissection. Journal of Immunology Research (PubMed: 36132983) [IF=4.1]

Application: WB    Species: Rat    Sample: VSMCs

Figure 4 The impact of C/EBPα in angiotensin II- (Ang II-) treated VSMCs. The relative protein expressions in Ang II-treated VSMCs with presence of C/EBPα overexpression or knockdown were analyzed by Western blot. GAPDH was used as an internal control. Data are presented as means ± SEM. (a, b) Evaluation of the influence of C/EBPα on the expression of α-SMA, SM-MHC, and OPN proteins. (c, d) Evaluation of the influence of C/EBPα on the expression of PIK3C2A protein. (e, f) Evaluation of the influence of C/EBPα on the expression of Beclin-1, p62, LC3II, and LC3I proteins. (g, h) Evaluation of the influence of C/EBPα on the expression of MMP-2 and MMP-9 proteins. (i) Intracellular ROS was measured by flow cytometry using DCFH-DA probe with presence of C/EBPα overexpression or knockdown. Control: control group; model: treated with 1 μM Ang II; vector: treated with 1 μM Ang II in vector transfected VSMCs; pcC/EBPα: treated with 1 μM Ang II in C/EBPα-overexpressed VSMCs; siNC: treated with 1 μM Ang II in siRNA NC-transfected VSMCs; siC/EBPα: treated with 1 μM Ang II in C/EBPα-knockdown VSMCs. ∗P < 0.05 vs. control,

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