MYBBP1A Antibody | Affinity Biosciences

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MYBBP1A Antibody - Western blot analysis of extracts from Hela cells(heat shock treatment), using MYBBP1A Antibody.

MYBBP1A Antibody - DF8468 staining A549 cells by IF/ICC.

Product:	MYBBP1A Antibody
Catalog:	DF8468
Description:	Rabbit polyclonal antibody to MYBBP1A
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Rabbit
Mol.Wt.:	149 kDa; 149kD(Calculated).
Uniprot:	Q9BQG0
RRID:	AB_2841700

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Rabbit(89%)

Clonality:

Polyclonal

Specificity:

MYBBP1A Antibody detects endogenous levels of total MYBBP1A.

RRID:

AB_2841700
Cite Format: Affinity Biosciences Cat# DF8468, RRID:AB_2841700.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AL024407; AU019902; cb486; FLJ37886; MBB1A_HUMAN; MYB binding protein (P160) 1a; myb binding protein (P160) 1a like; Myb-binding protein 1A; Mybbp1a; nuclear protein P160; P160; p160MBP; p53 activated protein 2; p67MBP; PAP2; PAR interacting protein; RP23 48A2.3; sb:cb486;

Immunogens

Immunogen:

Uniprot:

Q9BQG0(MBB1A_HUMAN) >>Visit HPA database.

Gene(ID):

MYBBP1A(10514)

Sequence:

Q9BQG0 MBB1A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MESRDPAQPMSPGEATQSGARPADRYGLLKHSREFLDFFWDIAKPEQETRLAATEKLLEYLRGRPKGSEMKYALKRLITGLGVGRETARPCYSLALAQLLQSFEDLPLCSILQQIQEKYDLHQVKKAMLRPALFANLFGVLALFQSGRLVKDQEALMKSVKLLQALAQYQNHLQEQPRKALVDILSEVSKATLQEILPEVLKADLNIILSSPEQLELFLLAQQKVPSKLKKLVGSVNLFSDENVPRLVNVLKMAASSVKKDRKLPAIALDLLRLALKEDKFPRFWKEVVEQGLLKMQFWPASYLCFRLLGAALPLLTKEQLHLVMQGDVIRHYGEHVCTAKLPKQFKFAPEMDDYVGTFLEGCQDDPERQLAVLVAFSSVTNQGLPVTPTFWRVVRFLSPPALQGYVAWLRAMFLQPDLDSLVDFSTNNQKKAQDSSLHMPERAVFRLRKWIIFRLVSIVDSLHLEMEEALTEQVARFCLFHSFFVTKKPTSQIPETKHPFSFPLENQAREAVSSAFFSLLQTLSTQFKQAPGQTQGGQPWTYHLVQFADLLLNHSHNVTTVTPFTAQQRQAWDRMLQTLKELEAHSAEARAAAFQHLLLLVGIHLLKSPAESCDLLGDIQTCIRKSLGEKPRRSRTKTIDPQEPPWVEVLVEILLALLAQPSHLMRQVARSVFGHICSHLTPRALQLILDVLNPETSEDENDRVVVTDDSDERRLKGAEDKSEEGEDNRSSESEEESEGEESEEEERDGDVDQGFREQLMTVLQAGKALGGEDSENEEELGDEAMMALDQSLASLFAEQKLRIQARRDEKNKLQKEKALRRDFQIRVLDLVEVLVTKQPENALVLELLEPLLSIIRRSLRSSSSKQEQDLLHKTARIFTHHLCRARRYCHDLGERAGALHAQVERLVQQAGRQPDSPTALYHFNASLYLLRVLKGNTAEGCVHETQEKQKAGTDPSHMPTGPQAASCLDLNLVTRVYSTALSSFLTKRNSPLTVPMFLSLFSRHPVLCQSLLPILVQHITGPVRPRHQACLLLQKTLSMREVRSCFEDPEWKQLMGQVLAKVTENLRVLGEAQTKAQHQQALSSLELLNVLFRTCKHEKLTLDLTVLLGVLQGQQQSLQQGAHSTGSSRLHDLYWQAMKTLGVQRPKLEKKDAKEIPSATQSPISKKRKKKGFLPETKKRKKRKSEDGTPAEDGTPAATGGSQPPSMGRKKRNRTKAKVPAQANGTPTTKSPAPGAPTRSPSTPAKSPKLQKKNQKPSQVNGAPGSPTEPAGQKQHQKALPKKGVLGKSPLSALARKKARLSLVIRSPSLLQSGAKKKAQVRKAGKP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Dog

Pig

Sheep

Bovine

Xenopus

Horse

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BQG0 As Substrate

Q9BQG0

Site	PTM Type	Enzyme	Source
S11	Phosphorylation		Uniprot
T16	Phosphorylation		Uniprot
S18	Phosphorylation		Uniprot
Y26	Phosphorylation		Uniprot
K30	Methylation		Uniprot
K30	Ubiquitination		Uniprot
S32	Phosphorylation		Uniprot
K44	Ubiquitination		Uniprot
K56	Ubiquitination		Uniprot
Y60	Phosphorylation		Uniprot
K71	Acetylation		Uniprot
K71	Ubiquitination		Uniprot
T79	Phosphorylation		Uniprot
K125	Ubiquitination		Uniprot
K151	Ubiquitination		Uniprot
K158	Acetylation		Uniprot
K158	Ubiquitination		Uniprot
K161	Ubiquitination		Uniprot
K179	Ubiquitination		Uniprot
S186	Phosphorylation		Uniprot
S189	Phosphorylation		Uniprot
K231	Ubiquitination		Uniprot
K252	Ubiquitination		Uniprot
K277	Ubiquitination		Uniprot
K341	Ubiquitination		Uniprot
S399	Phosphorylation		Uniprot
K432	Ubiquitination		Uniprot
T487	Phosphorylation		Uniprot
S492	Phosphorylation		Uniprot
K498	Ubiquitination		Uniprot
K581	Ubiquitination		Uniprot
R591	Methylation		Uniprot
S613	Phosphorylation		Uniprot
K631	Acetylation		Uniprot
K631	Ubiquitination		Uniprot
S635	Phosphorylation		Uniprot
S663	Phosphorylation		Uniprot
T708	Phosphorylation		Uniprot
S711	Phosphorylation		Uniprot
S775	Phosphorylation		Uniprot
S792	Phosphorylation		Uniprot
S795	Phosphorylation		Uniprot
S859	Phosphorylation		Uniprot
S862	Phosphorylation		Uniprot
S863	Phosphorylation		Uniprot
K866	Ubiquitination		Uniprot
K874	Ubiquitination		Uniprot
R896	Methylation		Uniprot
K935	Ubiquitination		Uniprot
T946	Phosphorylation		Uniprot
K949	Ubiquitination		Uniprot
Y978	Phosphorylation		Uniprot
T987	Phosphorylation		Uniprot
K988	Ubiquitination		Uniprot
T994	Phosphorylation		Uniprot
S1000	Phosphorylation		Uniprot
S1003	Phosphorylation		Uniprot
S1011	Phosphorylation		Uniprot
T1021	Phosphorylation		Uniprot
C1031	S-Nitrosylation		Uniprot
K1036	Ubiquitination		Uniprot
K1053	Ubiquitination		Uniprot
K1062	Ubiquitination		Uniprot
K1140	Ubiquitination		Uniprot
K1148	Ubiquitination		Uniprot
K1155	Ubiquitination		Uniprot
S1159	Phosphorylation		Uniprot
T1161	Phosphorylation		Uniprot
S1163	Phosphorylation		Uniprot
S1166	Phosphorylation		Uniprot
S1186	Phosphorylation		Uniprot
T1190	Phosphorylation		Uniprot
T1196	Phosphorylation		Uniprot
T1200	Phosphorylation		Uniprot
S1203	Phosphorylation		Uniprot
S1207	Phosphorylation		Uniprot
T1227	Phosphorylation		Uniprot
T1229	Phosphorylation		Uniprot
S1232	Phosphorylation		Uniprot
T1239	Phosphorylation		Uniprot
S1241	Phosphorylation		Uniprot
S1243	Phosphorylation		Uniprot
T1244	Phosphorylation		Uniprot
S1248	Phosphorylation		Uniprot
S1267	Phosphorylation	P28482 (MAPK1)	Uniprot
T1269	Phosphorylation		Uniprot
S1290	Phosphorylation		Uniprot
S1293	Phosphorylation		Uniprot
S1303	Phosphorylation	Q96GD4 (AURKB) , O14965 (AURKA)	Uniprot
S1308	Phosphorylation		Uniprot
S1310	Phosphorylation	O14757 (CHEK1)	Uniprot
S1314	Phosphorylation		Uniprot
K1317	Acetylation		Uniprot
K1319	Acetylation		Uniprot

Research Backgrounds

Q9BQG0_MBB1A_HUMAN

Function:

May activate or repress transcription via interactions with sequence specific DNA-binding proteins (By similarity). Repression may be mediated at least in part by histone deacetylase activity (HDAC activity) (By similarity). Acts as a corepressor and in concert with CRY1, represses the transcription of the core circadian clock component PER2 (By similarity). Preferentially binds to dimethylated histone H3 'Lys-9' (H3K9me2) on the PER2 promoter (By similarity). Has a role in rRNA biogenesis together with PWP1.

PTMs:

Citrullinated by PADI4.

Subcellular Location:

Cytoplasm. Nucleus. Nucleus>Nucleolus.
Note: Shuttles between the nucleus and cytoplasm. Nuclear import may be mediated by KPNA2, while export appears to depend partially on XPO1/CRM1 (By similarity). Predominantly nucleolar.

Subunit Structure:

Binds to and represses JUN and MYB via the leucine zipper regions present in these proteins. Also binds to and represses PPARGC1A: this interaction is abrogated when PPARGC1A is phosphorylated by MAPK1/ERK. Binds to and stimulates transcription by AHR. Binds to KPNA2. Interacts with CLOCK and CRY1 (By similarity). Component of the B-WICH complex, at least composed of SMARCA5/SNF2H, BAZ1B/WSTF, SF3B1, DEK, MYO1C, ERCC6, MYBBP1A and DDX21.

Family&Domains:

Belongs to the MYBBP1A family.

Restrictive clause

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For Research Use Only.
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MYBBP1A Antibody - #DF8468