ENT1 Antibody - N-terminal - DF8542
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- Product Name
- ENT1 Antibody
-
- Catalog No.
- DF8542
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- RRID
- AB_2841746
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- Source
- Rabbit
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- Application
- WB,IHC,ELISA
-
- Reactivity
- Human, Mouse, Rat
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- Prediction
- Pig(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%)
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- UniProt
-
- Mol.Wt
- 50kD;
50kDa(Calculated). -
- Concentration
- 1mg/ml
Related Products
Product Information
Alternative Names:Expand▼
Equilibrative NBMPR-sensitive nucleoside transporter; equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative nucleoside transporter 1; es-type; MGC1465; MGC3778; Nucleoside transporter; Nucleoside transporter, es-type; OTTHUMP00000016506; OTTHUMP00000016507; OTTHUMP00000016508; OTTHUMP00000016509; OTTHUMP00000016510; OTTHUMP00000016511; OTTHUMP00000016512; S29A1_HUMAN; Slc29a1; solute carrier family 29 (equilibrative nucleoside transporter), member 1; solute carrier family 29 (nucleoside transporters), member 1; Solute carrier family 29 member 1;
Applications:
WB 1:1000-3000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human, Mouse, Rat
Predicted Reactivity:
Pig(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%)
Source:
Rabbit
Clonality:
Polyclonal
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Specificity:
ENT1 Antibody detects endogenous levels of total ENT1.
RRID:
AB_2841746
Please cite this product as: Affinity Biosciences Cat# DF8542, RRID:AB_2841746.
Format:
Liquid
Concentration:
1mg/ml
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Immunogen Information
Immunogen:
A synthesized peptide derived from human ENT1, corresponding to a region within N-terminal amino acids.
Uniprot:
>>Visit The Human Protein Atlas
Gene ID:
Gene Name:
SLC29A1
Molecular Weight:
Observed Mol.Wt.: 50kD.
Predicted Mol.Wt.: 50kDa(Calculated)..
Subcellular Location:
Basolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells.
Tissue Specificity:
Q99808 S29A1_HUMAN:
Detected in erythrocytes (at protein level). Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.
Sequence:
- Q99808 S29A1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTSHQPQDRYKAVWLIFFMLGLGTLLPWNFFMTATQYFTNRLDMSQNVSLVTAELSKDAQASAAPAAPLPERNSLSAIFNNVMTLCAMLPLLLFTYLNSFLHQRIPQSVRILGSLVAILLVFLITAILVKVQLDALPFFVITMIKIVLINSFGAILQGSLFGLAGLLPASYTAPIMSGQGLAGFFASVAMICAIASGSELSESAFGYFITACAVIILTIICYLGLPRLEFYRYYQQLKLEGPGEQETKLDLISKGEEPRAGKEESGVSVSNSQPTNESHSIKAILKNISVLAFSVCFIFTITIGMFPAVTVEVKSSIAGSSTWERYFIPVSCFLTFNIFDWLGRSLTAVFMWPGKDSRWLPSLVLARLVFVPLLLLCNIKPRRYLTVVFEHDAWFIFFMAAFAFSNGYLASLCMCFGPKKVKPAEAETAGAIMAFFLCLGLALGAVFSFLFRAIV
Research Background
Function:
Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).
Post-translational Modifications:
Glycosylated.
Subcellular Location:
Basolateral cell membrane>Multi-pass membrane protein. Apical cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Note: Predominantly localized in the basolateral membrane in polarized MDCK cells.
Tissue Specificity:
Detected in erythrocytes (at protein level). Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.
Subunit Structure:
Identified in a complex with STOM.
Similarity:
Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.
Research Fields
Research Fields:
· Human Diseases > Substance dependence > Alcoholism.
Protocols & Troubleshooting
WB Images(2)
IHC Images(2)
Reference Citations:
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Catalog Number :
DF8542-BP
(Blocking peptide available as DF8542-BP)
Price/Size :
$350/1mg.
Tips: For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).
Function :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.
Format and storage :
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions :
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Q99808 as Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N48 | N-Glycosylation | Uniprot | |
| S63 | O-Glycosylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| T143 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| T248 | Phosphorylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| S271 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Q05655 (PRKCD) | Uniprot |
| K283 | Ubiquitination | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| K381 | Ubiquitination | Uniprot |
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