ENT1 Antibody - DF8542 - affinity

	Price	Size
	$280	100ul
	$350	200ul

Product Information

Alternative Names:Expand▼

Equilibrative NBMPR-sensitive nucleoside transporter; equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative nucleoside transporter 1; es-type; MGC1465; MGC3778; Nucleoside transporter; Nucleoside transporter, es-type; OTTHUMP00000016506; OTTHUMP00000016507; OTTHUMP00000016508; OTTHUMP00000016509; OTTHUMP00000016510; OTTHUMP00000016511; OTTHUMP00000016512; S29A1_HUMAN; Slc29a1; solute carrier family 29 (equilibrative nucleoside transporter), member 1; solute carrier family 29 (nucleoside transporters), member 1; Solute carrier family 29 member 1;

Applications:

WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

ENT1 Antibody detects endogenous levels of total ENT1.

RRID:

AB_2841746
Please cite this product as: Affinity Biosciences Cat# DF8542, RRID:AB_2841746.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human ENT1, corresponding to a region within N-terminal amino acids.

Uniprot:

>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

SLC29A1

Molecular Weight:

Observed Mol.Wt.: 50kD.
Predicted Mol.Wt.: 50kDa(Calculated)..

Subcellular Location:

Basolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells.

Tissue Specificity:

Q99808 S29A1_HUMAN:
Detected in erythrocytes (at protein level). Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.

Sequence:

Q99808 S29A1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTTSHQPQDRYKAVWLIFFMLGLGTLLPWNFFMTATQYFTNRLDMSQNVSLVTAELSKDAQASAAPAAPLPERNSLSAIFNNVMTLCAMLPLLLFTYLNSFLHQRIPQSVRILGSLVAILLVFLITAILVKVQLDALPFFVITMIKIVLINSFGAILQGSLFGLAGLLPASYTAPIMSGQGLAGFFASVAMICAIASGSELSESAFGYFITACAVIILTIICYLGLPRLEFYRYYQQLKLEGPGEQETKLDLISKGEEPRAGKEESGVSVSNSQPTNESHSIKAILKNISVLAFSVCFIFTITIGMFPAVTVEVKSSIAGSSTWERYFIPVSCFLTFNIFDWLGRSLTAVFMWPGKDSRWLPSLVLARLVFVPLLLLCNIKPRRYLTVVFEHDAWFIFFMAAFAFSNGYLASLCMCFGPKKVKPAEAETAGAIMAFFLCLGLALGAVFSFLFRAIV

Research Background

Q99808_S29A1_HUMAN

Function:

Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).

Post-translational Modifications:

Glycosylated.

Subcellular Location:

Basolateral cell membrane>Multi-pass membrane protein. Apical cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Note: Predominantly localized in the basolateral membrane in polarized MDCK cells.

Tissue Specificity:

Detected in erythrocytes (at protein level). Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.

Subunit Structure:

Identified in a complex with STOM.

Similarity:

Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.

Research Fields

Research Fields:

· Human Diseases > Substance dependence > Alcoholism.

Protocols & Troubleshooting

Sample Preparation For Western Blotting.

Western Blotting Handbook.

WB Images(1)

Western blot analysis of ENT1 using Jurkat whole cell lysates

Reference Citations:

1). Liu P;Zhu L;Zhou L;Bai X; et al. e5NT inhibitor protects acute restraint stress‐induced depression by regulating nucleoside release in mice. J Pharm Pharmacol 2020 Aug 2. (PubMed: 32743802)

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Results:

Application

Sample

Antibody dilution ratio

Result image

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Content

Verification code

Catalog Number :

DF8542-BP
(Blocking peptide available as DF8542-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Rabbit

100%

Chicken

100%

Sheep

100%

Bovine

100%

Horse

100%

Pig

90%

Xenopus

50%

Dog

Zebrafish

High similarity Medium similarity Low similarity No similarity

Q99808 as Substrate

Q99808

Site	PTM Type	Enzyme	Source
N48	N-Glycosylation		Uniprot
S63	O-Glycosylation		Uniprot
S63	Phosphorylation		Uniprot
T143	Phosphorylation		Uniprot
K239	Ubiquitination		Uniprot
T248	Phosphorylation		Uniprot
K249	Ubiquitination		Uniprot
S254	Phosphorylation		Uniprot
K255	Ubiquitination		Uniprot
K263	Ubiquitination		Uniprot
S266	Phosphorylation		Uniprot
S269	Phosphorylation		Uniprot
S271	Phosphorylation		Uniprot
S273	Phosphorylation		Uniprot
S281	Phosphorylation	Q05655 (PRKCD)	Uniprot
K283	Ubiquitination		Uniprot
K287	Ubiquitination		Uniprot
K356	Ubiquitination		Uniprot
K381	Ubiquitination		Uniprot

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

Secondary antibody Goat Anti-Rabbit IgG (H+L) HRP (S0001) Goat Anti-Rabbit IgG (H+L) AP (S0003) Goat Anti-Rabbit IgG (H+L) Fluor594-conjugated (S0006) Goat Anti-Rabbit IgG (H+L) FITC-conjugated (S0008) Goat Anti-Rabbit IgG (H+L) CY3-conjugated (S0011) Goat Anti-Rabbit IgG (H+L) Fluor647-conjugated (S0013) Goat Anti-Rabbit IgG (H+L) TRITC-conjugated (S0015) Goat Anti-Rabbit IgG (H+L) Fluor488-conjugated (S0018)

Home / Primary antibody / ENT1 Antibody

ENT1 Antibody - N-terminal - DF8542

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Product Information

Alternative Names:Expand▼

Applications:

Reactivity:

Predicted Reactivity:

Source:

Clonality:

Purification:

Specificity:

RRID:

Format:

Concentration:

Storage Condition and Buffer:

Immunogen Information

Immunogen:

Uniprot:

Gene ID:

Gene Name:

Molecular Weight:

Subcellular Location:

Tissue Specificity:

Sequence:

Research Background

Function:

Post-translational Modifications:

Subcellular Location:

Tissue Specificity:

Subunit Structure:

Similarity:

Research Fields

Research Fields:

Protocols & Troubleshooting

WB Images(1)

Reference Citations:

Submit Review

Catalog Number :

Price/Size :

Function :

Format and storage :

Precautions :

Q99808 as Substrate

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