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  • Product Name
    ENT1 Antibody
  • Catalog No.
    DF8542
  • RRID
    AB_2841746
  • Source
    Rabbit
  • Application
    WB,ELISA
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%)
  • UniProt
  • Mol.Wt
    50kD;
    50kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

Equilibrative NBMPR-sensitive nucleoside transporter; equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter; Equilibrative nucleoside transporter 1; es-type; MGC1465; MGC3778; Nucleoside transporter; Nucleoside transporter, es-type; OTTHUMP00000016506; OTTHUMP00000016507; OTTHUMP00000016508; OTTHUMP00000016509; OTTHUMP00000016510; OTTHUMP00000016511; OTTHUMP00000016512; S29A1_HUMAN; Slc29a1; solute carrier family 29 (equilibrative nucleoside transporter), member 1; solute carrier family 29 (nucleoside transporters), member 1; Solute carrier family 29 member 1;

Applications:

WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

ENT1 Antibody detects endogenous levels of total ENT1.

RRID:

AB_2841746
Please cite this product as: Affinity Biosciences Cat# DF8542, RRID:AB_2841746.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human ENT1, corresponding to a region within N-terminal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

SLC29A1

Molecular Weight:

Observed Mol.Wt.: 50kD.
Predicted Mol.Wt.: 50kDa(Calculated)..

Subcellular Location:

Basolateral cell membrane. Apical cell membrane. Predominantly localized in the basolateral membrane in polarised MDCK cells.

Tissue Specificity:

Q99808 S29A1_HUMAN:
Detected in erythrocytes (at protein level). Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.

Sequence:
MTTSHQPQDRYKAVWLIFFMLGLGTLLPWNFFMTATQYFTNRLDMSQNVSLVTAELSKDAQASAAPAAPLPERNSLSAIFNNVMTLCAMLPLLLFTYLNSFLHQRIPQSVRILGSLVAILLVFLITAILVKVQLDALPFFVITMIKIVLINSFGAILQGSLFGLAGLLPASYTAPIMSGQGLAGFFASVAMICAIASGSELSESAFGYFITACAVIILTIICYLGLPRLEFYRYYQQLKLEGPGEQETKLDLISKGEEPRAGKEESGVSVSNSQPTNESHSIKAILKNISVLAFSVCFIFTITIGMFPAVTVEVKSSIAGSSTWERYFIPVSCFLTFNIFDWLGRSLTAVFMWPGKDSRWLPSLVLARLVFVPLLLLCNIKPRRYLTVVFEHDAWFIFFMAAFAFSNGYLASLCMCFGPKKVKPAEAETAGAIMAFFLCLGLALGAVFSFLFRAIV

Research Background

Function:

Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).

Post-translational Modifications:

Glycosylated.

Subcellular Location:

Basolateral cell membrane>Multi-pass membrane protein. Apical cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Note: Predominantly localized in the basolateral membrane in polarized MDCK cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Detected in erythrocytes (at protein level). Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.

Subunit Structure:

Identified in a complex with STOM.

Similarity:

Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.

Research Fields

Research Fields:

· Human Diseases > Substance dependence > Alcoholism.

Reference Citations:

1). Liu P;Zhu L;Zhou L;Bai X; et al. e5NT inhibitor protects acute restraint stress‐induced depression by regulating nucleoside release in mice. J Pharm Pharmacol 2020 Aug 2. (PubMed: 32743802)

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Catalog Number :

DF8542-BP
(Blocking peptide available as DF8542-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Rabbit
100%
Chicken
100%
Sheep
100%
Bovine
100%
Horse
100%
Pig
90%
Xenopus
50%
Dog
0%
Zebrafish
0%
High similarity Medium similarity Low similarity No similarity
Q99808 as Substrate
Site PTM Type Enzyme
N48 N-Glycosylation
S63 O-Glycosylation
S63 Phosphorylation
T143 Phosphorylation
K239 Ubiquitination
T248 Phosphorylation
K249 Ubiquitination
S254 Phosphorylation
K255 Ubiquitination
K263 Ubiquitination
S266 Phosphorylation
S269 Phosphorylation
S271 Phosphorylation
S273 Phosphorylation
S281 Phosphorylation Q05655 (PRKCD)
K283 Ubiquitination
K287 Ubiquitination
K356 Ubiquitination
K381 Ubiquitination
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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