RAP1 Antibody - #DF8598

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Product: RAP1 Antibody
Catalog: DF8598
Description: Rabbit polyclonal antibody to RAP1
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 44 kDa; 44kD(Calculated).
Uniprot: Q9NYB0
RRID: AB_2841802

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
RAP1 Antibody detects endogenous levels of total RAP1.
RRID:
AB_2841802
Cite Format: Affinity Biosciences Cat# DF8598, RRID:AB_2841802.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Dopamine receptor interacting protein 5; Dopamine receptor-interacting protein 5; DRIP 5; DRIP5; hRap1; MGC105533; RAP 1; RAP1 homolog; RAP1, yeast, homolog if; RAP1, yeast, homolog of; Repressor/activator protein 1 homolog; TE2IP_HUMAN; Telomeric Repeat Binding Factor 2 Interacting Protein; Telomeric repeat-binding factor 2-interacting protein 1; TERF2-interacting protein; TERF2-interacting telomeric protein 1; TERF2IP; TRF2 Interacting Telomeric Protein RAP1; TRF2 interacting telomeric RAP1 protein; TRF2-interacting telomeric protein 1; TRF2-interacting telomeric protein;

Immunogens

Immunogen:
Uniprot:

Q9NYB0(TE2IP_HUMAN) >>Visit HPA database.

Gene(ID):
TERF2IP(54386)
Expression:
Q9NYB0 TE2IP_HUMAN:

Ubiquitous. Highly expressed.

Sequence:
MAEAMDLGKDPNGPTHSSTLFVRDDGSSMSFYVRPSPAKRRLSTLILHGGGTVCRVQEPGAVLLAQPGEALAEASGDFISTQYILDCVERNERLELEAYRLGPASAADTGSEAKPGALAEGAAEPEPQRHAGRIAFTDADDVAILTYVKENARSPSSVTGNALWKAMEKSSLTQHSWQSLKDRYLKHLRGQEHKYLLGDAPVSPSSQKLKRKAEEDPEAADSGEPQNKRTPDLPEEEYVKEEIQENEEAVKKMLVEATREFEEVVVDESPPDFEIHITMCDDDPPTPEEDSETQPDEEEEEEEEKVSQPEVGAAIKIIRQLMEKFNLDLSTVTQAFLKNSGELEATSAFLASGQRADGYPIWSRQDDIDLQKDDEDTREALVKKFGAQNVARRIEFRKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
57
Horse
0
Xenopus
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NYB0 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T15 Phosphorylation
Y32 Phosphorylation
S36 Phosphorylation
S43 Phosphorylation
T44 Phosphorylation
T52 Phosphorylation
C54 S-Nitrosylation
S105 Phosphorylation
T109 Phosphorylation
K114 Ubiquitination
K149 Ubiquitination
S154 Phosphorylation
S156 Phosphorylation
K169 Ubiquitination
S176 Phosphorylation
S179 Phosphorylation
K194 Ubiquitination
Y195 Phosphorylation
S203 Phosphorylation P24941 (CDK2)
S205 Phosphorylation
S206 Phosphorylation
K208 Acetylation
K208 Ubiquitination
S222 Phosphorylation
K228 Ubiquitination
T230 Phosphorylation
Y238 Phosphorylation
K240 Sumoylation
K240 Ubiquitination
K251 Ubiquitination
K252 Ubiquitination
T286 Phosphorylation
S291 Phosphorylation
K316 Ubiquitination
T377 Phosphorylation
K384 Ubiquitination

Research Backgrounds

Function:

Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. Instead, it is required to negatively regulate telomere recombination and is essential for repressing homology-directed repair (HDR), which can affect telomere length. Does not bind DNA directly: recruited to telomeric double-stranded 5'-TTAGGG-3' repeats via its interaction with TERF2. Independently of its function in telomeres, also acts as a transcription regulator: recruited to extratelomeric 5'-TTAGGG-3' sites via its association with TERF2 or other factors, and regulates gene expression. When cytoplasmic, associates with the I-kappa-B-kinase (IKK) complex and acts as a regulator of the NF-kappa-B signaling by promoting IKK-mediated phosphorylation of RELA/p65, leading to activate expression of NF-kappa-B target genes.

Subcellular Location:

Nucleus. Cytoplasm. Chromosome. Chromosome>Telomere.
Note: Associates with chromosomes, both at telomeres and in extratelomeric sites. Also exists as a cytoplasmic form, where it associates with the IKK complex.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Highly expressed.

Subunit Structure:

Associates with the I-kappa-B-kinase (IKK) core complex, composed of CHUK, IKBKB and IKBKG (By similarity). Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP ACD and POT1. Interacts with TERF2; the interaction is direct. Does not interact with TERF1. Interacts with SLX4/BTBD12.

Family&Domains:

Belongs to the RAP1 family.

References

1). Epac1 signaling pathway mediates the damage and apoptosis of inner ear hair cells after noise exposure in a rat model. NEUROSCIENCE (PubMed: 33838290) [IF=3.3]

Application: WB    Species: rat    Sample: HEI-OC1 cells

Fig. 6. Expression of Epac1 in HEI-OC1 cells after oligomycin exposure. (A) Representative fluorescent microscopic images showing Epac1 expression in HEI-OC1 cells 12 h after oligomycin exposure; blue: DAPI; scale bar = 20 mm, n = 3. (B) Bar graph representing the intensity of Epac1 signal in HEI-OC1 cells. (C) Western blot analysis of Epac1 and Rap1 expression in HEI-OC1 cells 12 h after oligomycin exposure. b-actin was used as the loading control. The data represent the mean ± SEM, n = 3; *P < 0.05 vs. Control group, #P < 0.05 vs. OA.

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