RNF6 Antibody - #DF8890

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Product: RNF6 Antibody
Catalog: DF8890
Description: Rabbit polyclonal antibody to RNF6
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Horse
Mol.Wt.: 78kda; 78kD(Calculated).
Uniprot: Q9Y252
RRID: AB_2842086

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Horse(80%)
Clonality:
Polyclonal
Specificity:
RNF6 Antibody detects endogenous levels of total RNF6.
RRID:
AB_2842086
Cite Format: Affinity Biosciences Cat# DF8890, RRID:AB_2842086.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

E3 ubiquitin-protein ligase RNF6; Ring finger protein (C3H2C3 type) 6; Ring finger protein 6; RING H2 protein; RING H2 protein RNF6; RNF 6; RNF6; RNF6_HUMAN; SPG2;

Immunogens

Immunogen:
Uniprot:

Q9Y252(RNF6_HUMAN) >>Visit HPA database.

Gene(ID):
RNF6(6049)
Expression:
Q9Y252 RNF6_HUMAN:

Weakly expressed in peripheral blood, spleen, prostate, testis and ovary. According to PubMed:18368307, it is preferentially expressed in testis and ovary and hardly detected in other tissues.

Sequence:
MNQSRSRSDGGSEETLPQDHNHHENERRWQQERLHREEAYYQFINELNDEDYRLMRDHNLLGTPGEITSEELQQRLDGVKEQLASQPDLRDGTNYRDSEVPRESSHEDSLLEWLNTFRRTGNATRSGQNGNQTWRAVSRTNPNNGEFRFSLEIHVNHENRGFEIHGEDYTDIPLSDSNRDHTANRQQRSTSPVARRTRSQTSVNFNGSSSNIPRTRLASRGQNPAEGSFSTLGRLRNGIGGAAGIPRANASRTNFSSHTNQSGGSELRQREGQRFGAAHVWENGARSNVTVRNTNQRLEPIRLRSTSNSRSRSPIQRQSGTVYHNSQRESRPVQQTTRRSVRRRGRTRVFLEQDRERERRGTAYTPFSNSRLVSRITVEEGEESSRSSTAVRRHPTITLDLQVRRIRPGENRDRDSIANRTRSRVGLAENTVTIESNSGGFRRTISRLERSGIRTYVSTITVPLRRISENELVEPSSVALRSILRQIMTGFGELSSLMEADSESELQRNGQHLPDMHSELSNLGTDNNRSQHREGSSQDRQAQGDSTEMHGENETTQPHTRNSDSRGGRQLRNPNNLVETGTLPILRLAHFFLLNESDDDDRIRGLTKEQIDNLSTRHYEHNSIDSELGKICSVCISDYVTGNKLRQLPCMHEFHIHCIDRWLSENCTCPICRQPVLGSNIANNG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
80
Dog
60
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y252 As Substrate

Site PTM Type Enzyme
S6 Phosphorylation
K80 Ubiquitination
T133 Phosphorylation
R220 Methylation
R236 Methylation
R247 Methylation
S340 Phosphorylation
Y364 Phosphorylation
T365 Phosphorylation
S368 Phosphorylation
S370 Phosphorylation
S387 Phosphorylation
T389 Phosphorylation
T459 Phosphorylation
T461 Phosphorylation
S468 Phosphorylation
S476 Phosphorylation
S546 Phosphorylation
T547 Phosphorylation
S597 Phosphorylation
K608 Ubiquitination
K644 Ubiquitination

Research Backgrounds

Function:

E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator.

Subcellular Location:

Nucleus. Cytoplasm. Cell projection>Axon. Nucleus>PML body.
Note: Localizes to the PML nuclear bodies in Sertoli cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Weakly expressed in peripheral blood, spleen, prostate, testis and ovary. According to it is preferentially expressed in testis and ovary and hardly detected in other tissues.

Subunit Structure:

Interacts with LIMK1 (By similarity). Interacts with AR.

Family&Domains:

Belongs to the RNF12 family.

Restrictive clause

 

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