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$350 200ul

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  • Product Name
    CYB5R4 Antibody
  • Catalog No.
    DF9025
  • RRID
    AB_2842221
  • Source
    Rabbit
  • Application
    WB,IHC,ELISA
  • Reactivity
    Human
  • Prediction
    Pig(92%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(92%)
  • UniProt
  • Mol.Wt
    59kD;
    59kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

b5+b5R; b5b5r; C79736; cb5/cb5R; Cb5cb5r; Cyb5r4; Cytochrome b type NAD(P)H oxidoreductase; Cytochrome b5 reductase 4; dJ676J13.1; Flavohemoprotein b5+b5R; Flavohemoprotein b5/b5R; N terminal cytochrome b5 and cytochrome b5 oxidoreductase domain containing protein; N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein; NADPH cytochrome B5 oxidoreductase; NB5R4_HUMAN; NCB5OR; RP4 676J13.1;

Applications:

WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human

Predicted Reactivity:

Pig(92%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(92%)

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

CYB5R4 Antibody detects endogenous levels of total CYB5R4.

RRID:

AB_2842221
Please cite this product as: Affinity Biosciences Cat# DF9025, RRID:AB_2842221.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information

Immunogen:

A synthesized peptide derived from human CYB5R4, corresponding to a region within N-terminal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

CYB5R4

Molecular Weight:

Observed Mol.Wt.: 59kD.
Predicted Mol.Wt.: 59kDa(Calculated)..

Subcellular Location:

Endoplasmic reticulum. Soluble protein.

Tissue Specificity:

Q7L1T6 NB5R4_HUMAN:
Widely expressed.

Sequence:
MLNVPSQSFPAPRSQQRVASGGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKGRLIEVTEEELKKHNKKDDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGSDGTELFDQVHRWVNYESMLKECLVGRMAIKPAVLKDYREEEKKVLNGMLPKSQVTDTLAKEGPSYPSYDWFQTDSLVTIAIYTKQKDINLDSIIVDHQNDSFRAETIIKDCLYLIHIGLSHEVQEDFSVRVVESVGKIEIVLQKKENTSWDFLGHPLKNHNSLIPRKDTGLYYRKCQLISKEDVTHDTRLFCLMLPPSTHLQVPIGQHVYLKLPITGTEIVKPYTPVSGSLLSEFKEPVLPNNKYIYFLIKIYPTGLFTPELDRLQIGDFVSVSSPEGNFKISKFQELEDLFLLAAGTGFTPMVKILNYALTDIPSLRKVKLMFFNKTEDDIIWRSQLEKLAFKDKRLDVEFVLSAPISEWNGKQGHISPALLSEFLKRNLDKSKVLVCICGPVPFTEQGVRLLHDLNFSKNEIHSFTA

Research Background

Function:

NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS). Reduces a variety of substrates in vitro, such as cytochrome c, feericyanide and methemoglobin.

Subcellular Location:

Endoplasmic reticulum.
Note: Soluble protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Widely expressed.

Similarity:

Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Research Fields

Research Fields:

· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.

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Catalog Number :

DF9025-BP
(Blocking peptide available as DF9025-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Rabbit
100%
Bovine
100%
Dog
100%
Horse
100%
Xenopus
92%
Pig
92%
Zebrafish
75%
Sheep
0%
Chicken
0%
High similarity Medium similarity Low similarity No similarity
Q7L1T6 as Substrate
Site PTM Type Enzyme
M1 Acetylation
S14 Phosphorylation
S154 Phosphorylation
T157 Phosphorylation
K277 Ubiquitination
Y326 Phosphorylation
T361 Phosphorylation
K442 Ubiquitination
S471 Phosphorylation
S476 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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