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$280 100ul
$350 200ul

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  • Product Name
    VEZF1 Antibody
  • Catalog No.
    DF9036
  • RRID
    AB_2842232
  • Source
    Rabbit
  • Application
    WB,ELISA(peptide)
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
  • UniProt
  • Mol.Wt
    57 kD;
    57kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

DB1; MGC82370; Putative transcription factor DB1; Vascular endothelial zinc finger 1; VEZF1; VEZF1_HUMAN; Zinc finger protein 161; ZNF161;

Applications:

WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

VEZF1 Antibody detects endogenous levels of total VEZF1.

RRID:

AB_2842232
Please cite this product as: Affinity Biosciences Cat# DF9036, RRID:AB_2842232.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human VEZF1, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

VEZF1

Molecular Weight:

Observed Mol.Wt.: 57 kD.
Predicted Mol.Wt.: 57kDa(Calculated)..

Subcellular Location:

Nucleus.

Tissue Specificity:

Q14119 VEZF1_HUMAN:
Ubiquitously expressed. Highest levels in skeletal muscle and kidney.

Sequence:
MEANWTAFLFQAHEASHHQQQAAQNSLLPLLSSAVEPPDQKPLLPIPITQKPQGAPETLKDAIGIKKEKPKTSFVCTYCSKAFRDSYHLRRHESCHTGIKLVSRPKKTPTTVVPLISTIAGDSSRTSLVSTIAGILSTVTTSSSGTNPSSSASTTAMPVTQSVKKPSKPVKKNHACEMCGKAFRDVYHLNRHKLSHSDEKPFECPICNQRFKRKDRMTYHVRSHEGGITKPYTCSVCGKGFSRPDHLSCHVKHVHSTERPFKCQTCTAAFATKDRLRTHMVRHEGKVSCNICGKLLSAAYITSHLKTHGQSQSINCNTCKQGISKTCMSEETSNQKQQQQQQQQQQQQQQQQQQHVTSWPGKQVETLRLWEEAVKARKKEAANLCQTSTAATTPVTLTTPFSITSSVSSGTMSNPVTVAAAMSMRSPVNVSSAVNITSPMNIGHPVTITSPLSMTSPLTLTTPVNLPTPVTAPVNIAHPVTITSPMNLPTPMTLAAPLNIAMRPVESMPFLPQALPTSPPW

Research Background

Function:

Possible transcription factor. Specifically binds to the CT/GC-rich region of the interleukin-3 promoter and mediates tax transactivation of IL-3.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Ubiquitously expressed. Highest levels in skeletal muscle and kidney.

Subunit Structure:

Interacts with ARHGAP22.

Similarity:

Belongs to the krueppel C2H2-type zinc-finger protein family.

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Catalog Number :

DF9036-BP
(Blocking peptide available as DF9036-BP)

Price/Size :

$350/1mg.
Tips: For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Horse
100%
Bovine
100%
Sheep
100%
Dog
100%
Xenopus
100%
Chicken
100%
Rabbit
100%
Zebrafish
0%
High similarity Medium similarity Low similarity No similarity
Q14119 as Substrate
Site PTM Type Enzyme
K41 Sumoylation
K66 Sumoylation
S86 Phosphorylation
S127 Phosphorylation
S130 Phosphorylation
S144 Phosphorylation
S150 Phosphorylation
T155 Phosphorylation
K181 Ubiquitination
S195 Phosphorylation
S197 Phosphorylation
K200 Ubiquitination
S256 Phosphorylation
K273 Ubiquitination
K286 Ubiquitination
S297 Phosphorylation
Y300 Phosphorylation
T302 Phosphorylation
S303 Phosphorylation
K320 Ubiquitination
K325 Ubiquitination
K362 Acetylation
K362 Ubiquitination
K375 Ubiquitination
T517 Phosphorylation
S518 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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