ADAMDEC1 Antibody | Affinity Biosciences

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Product:	ADAMDEC1 Antibody
Catalog:	DF9167
Description:	Rabbit polyclonal antibody to ADAMDEC1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	53kDa; 53kD(Calculated).
Uniprot:	O15204
RRID:	AB_2842363

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

ADAMDEC1 Antibody detects endogenous levels of total ADAMDEC1.

RRID:

AB_2842363
Cite Format: Affinity Biosciences Cat# DF9167, RRID:AB_2842363.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

A disintegrin and metalloproteinase domain like protein decysin 1; A disintegrin and metalloproteinase domain-like protein decysin-1; ADAM DEC1; ADAM like decysin 1; ADAM like protein decysin 1; ADAM-like protein decysin-1; ADAMDEC 1; Adamdec1; ADEC1_HUMAN; Decysin; Disintegrin protease; M12.219;

Immunogens

Immunogen:

Uniprot:

O15204(ADEC1_HUMAN) >>Visit HPA database.

Gene(ID):

ADAMDEC1(27299)

Expression:

O15204 ADEC1_HUMAN:

Expressed highly in the small intestine and appendix, moderately in lymph node, mucosal lining of the colon, thymus, spleen and very weakly in the bone marrow. Predominantly expressed in dendritic cells (DC) of the germinal center. Weakly expressed in monocyte and highly expressed in macrophage. Absent in immature DC.

Sequence:

O15204 ADEC1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLRGISQLPAVATMSWVLLPVLWLIVQTQAIAIKQTPELTLHEIVCPKKLHILHKREIKNNQTEKHGKEERYEPEVQYQMILNGEEIILSLQKTKHLLGPDYTETLYSPRGEEITTKPENMEHCYYKGNILNEKNSVASISTCDGLRGYFTHHHQRYQIKPLKSTDEKEHAVFTSNQEEQDPANHTCGVKSTDGKQGPIRISRSLKSPEKEDFLRAQKYIDLYLVLDNAFYKNYNENLTLIRSFVFDVMNLLNVIYNTIDVQVALVGMEIWSDGDKIKVVPSASTTFDNFLRWHSSNLGKKIHDHAQLLSGISFNNRRVGLAASNSLCSPSSVAVIEAKKKNNVALVGVMSHELGHVLGMPDVPFNTKCPSGSCVMNQYLSSKFPKDFSTSCRAHFERYLLSQKPKCLLQAPIPTNIMTTPVCGNHLLEVGEDCDCGSPKECTNLCCEALTCKLKPGTDCGGDAPNHTTE

PTMs - O15204 As Substrate

O15204

Site	PTM Type	Source
Y72	Phosphorylation	Uniprot
N184	N-Glycosylation	Uniprot
N237	N-Glycosylation	Uniprot

Research Backgrounds

O15204_ADEC1_HUMAN

Function:

May play an important role in the control of the immune response and during pregnancy.

Subcellular Location:

Secreted.

Tissue Specificity:

References

1). ADAMDEC1 promotes skin inflammation in rosacea via modulating the polarization of M1 macrophages. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2020 (PubMed: 31627897) [IF=2.5]

Application: IF/ICC Species: mouse Sample: macrophages

Fig. 2. ADAMDEC1 was located at macrophages and upregulated in M1 macrophages. (A) Co-localization of F4/80 and ADAMDEC1 in sections from LL37-induced mice model.

Application: WB Species: mouse Sample: macrophages

Fig. 3. |Knocking down of ADAMDEC1 impeded M1 macrophage activation. Macrophages were transfected with ADAMDEC1 siRNA (siAdam-1 and siAdam-2) (n ¼ 3) and negative control siRNA (siCtrl) (n ¼ 3) separately for 48 h. (A) ADAMDEC1 protein and mRNA level were detected in transfected human monocyte-derived macrophages.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ADAMDEC1 Antibody - #DF9167