SERPINF2 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from A2780 cells, using SERPINF2 Antibody.

Product:	SERPINF2 Antibody
Catalog:	DF9194
Description:	Rabbit polyclonal antibody to SERPINF2
Application:	WB IHC IF/ICC
Reactivity:	Human, Rat
Prediction:	Pig, Horse
Mol.Wt.:	55 kDa; 55kD(Calculated).
Uniprot:	P08697
RRID:	AB_2842390

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Pig(83%), Horse(83%)

Clonality:

Polyclonal

Specificity:

SERPINF2 Antibody detects endogenous levels of total SERPINF2.

RRID:

AB_2842390
Cite Format: Affinity Biosciences Cat# DF9194, RRID:AB_2842390.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

A2AP; A2AP_HUMAN; AAP; Alpha 2 antiplasmin; Alpha 2 antiplasmin pigment epithelium derived factor; Alpha 2 AP; ALPHA 2 PI; Alpha 2 plasmin inhibitor; Alpha 2 plasmin inhibitor deficiency; Alpha-2-antiplasmin; Alpha-2-AP; Alpha-2-PI; Alpha-2-plasmin inhibitor; Antiplasmin deficiency; API; Plasmin inhibitor deficiency; PLI; Serine (or cysteine) peptidase inhibitor, clade F, member 2; Serine (Or cysteine) peptidase inhibitor, clade F, member 2, isoform CRA_c; Serine (or cysteine) proteinase inhibitor, clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2; Serine (Or cysteine) proteinase inhibitor, clade F, member 2; Serine or cysteine peptidase inhibitor clade F member 2; Serpin F2; Serpin peptidase inhibitor clade F; Serpin peptidase inhibitor, clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2; SERPINF2;

Immunogens

Immunogen:

Uniprot:

P08697(A2AP_HUMAN) >>Visit HPA database.

Gene(ID):

SERPINF2(5345)

Expression:

P08697 A2AP_HUMAN:

Expressed by the liver and secreted in plasma.

Sequence:

P08697 A2AP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALLWGLLVLSWSCLQGPCSVFSPVSAMEPLGRQLTSGPNQEQVSPLTLLKLGNQEPGGQTALKSPPGVCSRDPTPEQTHRLARAMMAFTADLFSLVAQTSTCPNLILSPLSVALALSHLALGAQNHTLQRLQQVLHAGSGPCLPHLLSRLCQDLGPGAFRLAARMYLQKGFPIKEDFLEQSEQLFGAKPVSLTGKQEDDLANINQWVKEATEGKIQEFLSGLPEDTVLLLLNAIHFQGFWRNKFDPSLTQRDSFHLDEQFTVPVEMMQARTYPLRWFLLEQPEIQVAHFPFKNNMSFVVLVPTHFEWNVSQVLANLSWDTLHPPLVWERPTKVRLPKLYLKHQMDLVATLSQLGLQELFQAPDLRGISEQSLVVSGVQHQSTLELSEVGVEAAAATSIAMSRMSLSSFSVNRPFLFFIFEDTTGLPLFVGSVRNPNPSAPRELKEQQDSPGNKDFLQSLKGFPRGDKLFGPDLKLVPPMEEDYPQFGSPK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Dog

Rabbit

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08697 As Substrate

P08697

Site	PTM Type	Source
N126	N-Glycosylation	Uniprot
N295	N-Glycosylation	Uniprot
N309	N-Glycosylation	Uniprot
T397	Phosphorylation	Uniprot
S398	Phosphorylation	Uniprot
S405	Phosphorylation	Uniprot
S407	Phosphorylation	Uniprot
S410	Phosphorylation	Uniprot
K454	Ubiquitination	Uniprot
S459	Phosphorylation	Uniprot
Y484	Phosphorylation	Uniprot

Research Backgrounds

P08697_A2AP_HUMAN

Function:

Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.

PTMs:

Proteolytically cleaved at Pro-39 by both the prolyl endopeptidase FAP form and antiplasmin-cleaving enzyme FAP soluble form to generate mature alpha-2-antiplasmin.

Subcellular Location:

Secreted.

Tissue Specificity:

Expressed by the liver and secreted in plasma.

Subunit Structure:

Forms protease inhibiting heterodimer with TMPRSS7.

Family&Domains:

Belongs to the serpin family.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

SERPINF2 Antibody - #DF9194