APBA1 Antibody - #DF9202

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Product: APBA1 Antibody
Catalog: DF9202
Description: Rabbit polyclonal antibody to APBA1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 93kDa; 93kD(Calculated).
Uniprot: Q02410
RRID: AB_2842398

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(86%), Sheep(86%), Rabbit(86%), Dog(93%)
Clonality:
Polyclonal
Specificity:
APBA1 Antibody detects endogenous levels of total APBA1.
RRID:
AB_2842398
Cite Format: Affinity Biosciences Cat# DF9202, RRID:AB_2842398.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Adapter protein X11 alpha; Adapter protein X11alpha; Amyloid beta A4 precursor protein-binding family A member 1; Apba1; APBA1_HUMAN; Mint 1; Mint-1; Neuron specific X11 protein; Neuron-specific X11 protein; Neuronal Munc18 1 interacting protein 1; Neuronal Munc18-1-interacting protein 1; UROP11; x11; X11alpha;

Immunogens

Immunogen:
Uniprot:

Q02410(APBA1_HUMAN) >>Visit HPA database.

Gene(ID):
APBA1(320)
Expression:
Q02410 APBA1_HUMAN:

Brain and spinal cord. Isoform 2 is expressed in testis and brain, but not detected in lung, liver or spleen.

Sequence:
MNHLEGSAEVEVTDEAAGGEVNESVEADLEHPEVEEEQQQPPQQQHYVGRHQRGRALEDLRAQLGQEEEERGECLARSASTESGFHNHTDTAEGDVIAAARDGYDAERAQDPEDESAYAVQYRPEAEEYTEQAEAEHAEATHRRALPNHLHFHSLEHEEAMNAAYSGYVYTHRLFHRGEDEPYSEPYADYGGLQEHVYEEIGDAPELDARDGLRLYEQERDEAAAYRQEALGARLHHYDERSDGESDSPEKEAEFAPYPRMDSYEQEEDIDQIVAEVKQSMSSQSLDKAAEDMPEAEQDLERPPTPAGGRPDSPGLQAPAGQQRAVGPAGGGEAGQRYSKEKRDAISLAIKDIKEAIEEVKTRTIRSPYTPDEPKEPIWVMRQDISPTRDCDDQRPMDGDSPSPGSSSPLGAESSSTSLHPSDPVEASTNKESRKSLASFPTYVEVPGPCDPEDLIDGIIFAANYLGSTQLLSDKTPSKNVRMMQAQEAVSRIKMAQKLAKSRKKAPEGESQPMTEVDLFISTQRIKVLNADTQETMMDHPLRTISYIADIGNIVVLMARRRMPRSNSQENVEASHPSQDGKRQYKMICHVFESEDAQLIAQSIGQAFSVAYQEFLRANGINPEDLSQKEYSDLLNTQDMYNDDLIHFSKSENCKDVFIEKQKGEILGVVIVESGWGSILPTVIIANMMHGGPAEKSGKLNIGDQIMSINGTSLVGLPLSTCQSIIKGLKNQSRVKLNIVRCPPVTTVLIRRPDLRYQLGFSVQNGIICSLMRGGIAERGGVRVGHRIIEINGQSVVATPHEKIVHILSNAVGEIHMKTMPAAMYRLLTAQEQPVYI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
93
Bovine
86
Sheep
86
Rabbit
86
Pig
75
Xenopus
71
Horse
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q02410 As Substrate

Site PTM Type Enzyme
S78 Phosphorylation
S80 Phosphorylation
S83 Phosphorylation
T89 Phosphorylation
T91 Phosphorylation
Y118 Phosphorylation
Y122 Phosphorylation
Y129 Phosphorylation
S154 Phosphorylation
Y165 Phosphorylation
S166 Phosphorylation
Y168 Phosphorylation
Y170 Phosphorylation
T171 Phosphorylation
Y183 Phosphorylation
S184 Phosphorylation
Y190 Phosphorylation
Y198 Phosphorylation
Y216 Phosphorylation
Y226 Phosphorylation
Y238 Phosphorylation
S242 Phosphorylation
S246 Phosphorylation
S248 Phosphorylation
S263 Phosphorylation
Y264 Phosphorylation
S282 Phosphorylation
S285 Phosphorylation
T305 Phosphorylation
S313 Phosphorylation
S339 Phosphorylation
S347 Phosphorylation
Y369 Phosphorylation
T370 Phosphorylation
S386 Phosphorylation
S422 Phosphorylation
S428 Phosphorylation
S433 Phosphorylation
S566 Phosphorylation
S568 Phosphorylation

Research Backgrounds

Function:

Putative function in synaptic vesicle exocytosis by binding to Munc18-1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the amyloid-beta precursor protein (APP) and hence formation of APP-beta.

Subcellular Location:

Cytoplasm. Cytoplasm>Perinuclear region. Nucleus.
Note: Only about 5% of the protein is located in the nucleus.

Golgi apparatus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Brain and spinal cord. Isoform 2 is expressed in testis and brain, but not detected in lung, liver or spleen.

Subunit Structure:

Part of a multimeric complex containing Munc18-1 and syntaxin-1. Also part of the brain-specific heterotrimeric complex LIN-10/X11-alpha, LIN-2/CASK, and LIN7. Both isoform 1 and isoform 2 bind to the cytoplasmic domain of amyloid protein (APP). Interacts (via PDZ 1 and 2 domains) with FSPB. Isoform 2, but not isoform 1, interacts (via its truncated PID domain) with active, GTP-bound RAB6A and RAB6B.

Family&Domains:

Composed of an N-terminal domain that binds Munc18-1 and LIN-2/CASK, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the amyloid-beta precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.

The autoinhibitory helix linker occludes the APP binding site.

The PID domain, truncated by 11 amino acids, as observed in isoform 2, but not full-length, mediates the interaction with RAB6A and RAB6B.

Restrictive clause

 

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