APOL6 Antibody | Affinity Biosciences

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APOL6 Antibody - Western blot analysis of APOL6 using COLO205 whole cell lysates.

Product:	APOL6 Antibody
Catalog:	DF9219
Description:	Rabbit polyclonal antibody to APOL6
Application:	WB
Reactivity:	Human
Mol.Wt.:	38 kDa; 38kD(Calculated).
Uniprot:	Q9BWW8
RRID:	AB_2842415

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

APOL6 Antibody detects endogenous levels of total APOL6.

RRID:

AB_2842415
Cite Format: Affinity Biosciences Cat# DF9219, RRID:AB_2842415.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2310076O14Rik; APOL VI; ApoL-VI; APOL6; APOL6_HUMAN; Apolipoprotein L VI; Apolipoprotein L, 6; Apolipoprotein L-VI; Apolipoprotein L6; APOLVI; DKFZp667M075; FLJ38562; FLJ90164; MGC57495;

Immunogens

Immunogen:

Uniprot:

Q9BWW8(APOL6_HUMAN) >>Visit HPA database.

Gene(ID):

APOL6(80830)

Expression:

Q9BWW8 APOL6_HUMAN:

Widely expressed; highly expressed in the uterus, fetal brain and spinal cord, also detected in heart, liver, lung, colon, spleen, thymus, prostate, placenta, adrenal gland, salivary and mammary gland.

Sequence:

Q9BWW8 APOL6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDNQAERESEAGVGLQRDEDDAPLCEDVELQDGDLSPEEKIFLREFPRLKEDLKGNIDKLRALADDIDKTHKKFTKANMVATSTAVISGVMSLLGLALAPATGGGSLLLSTAGQGLATAAGVTSIVSGTLERSKNKEAQARAEDILPTYDQEDREDEEEKADYVTAAGKIIYNLRNTLKYAKKNVRAFWKLRANPRLANATKRLLTTGQVSSRSRVQVQKAFAGTTLAMTKNARVLGGVMSAFSLGYDLATLSKEWKHLKEGARTKFAEELRAKALELERKLTELTQLYKSLQQKVRSRARGVGKDLTGTCETEAYWKELREHVWMWLWLCVCLCVCVYVQFT

PTMs - Q9BWW8 As Substrate

Q9BWW8

Site	PTM Type	Source
K40	Ubiquitination	Uniprot
K59	Ubiquitination	Uniprot
K160	Ubiquitination	Uniprot
K169	Ubiquitination	Uniprot
K190	Ubiquitination	Uniprot
K202	Ubiquitination	Uniprot
T206	Phosphorylation	Uniprot
K220	Ubiquitination	Uniprot
K231	Ubiquitination	Uniprot
K266	Ubiquitination	Uniprot
K274	Acetylation	Uniprot
K274	Ubiquitination	Uniprot
K281	Ubiquitination	Uniprot
K290	Ubiquitination	Uniprot
K295	Ubiquitination	Uniprot
K305	Ubiquitination	Uniprot

Research Backgrounds

Q9BWW8_APOL6_HUMAN

Function:

May affect the movement of lipids in the cytoplasm or allow the binding of lipids to organelles.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Family&Domains:

Belongs to the apolipoprotein L family.

References

1). APOL6 predicts immunotherapy efficacy of bladder cancer by ferroptosis. BMC cancer, 2024 (PubMed: 39187773) [IF=3.8]

Application: IF/ICC Species: Human Sample: T24 cells

Fig. 6. APOL6 as a key regulatory factor in inducing ferroptosis in BLCA. A and B Relation between APOL6 and ferroptosis-related markers. C Levels of the Cox-2 and GPX4 protein in T24 cells following transfected with APOL6. D Lipid peroxidation in T24 cells was detected by C11-BODIPY581/591 probe. E T24 cells were transfected with APOL6, followed by detection with FerroOrange probe

Restrictive clause

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For Research Use Only.
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APOL6 Antibody - #DF9219