Product: BCL2L14 Antibody
Catalog: DF9220
Description: Rabbit polyclonal antibody to BCL2L14
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Bovine, Sheep
Mol.Wt.: 37 kDa; 37kD(Calculated).
Uniprot: Q9BZR8
RRID: AB_2842416

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(83%), Sheep(83%)
Clonality:
Polyclonal
Specificity:
BCL2L14 Antibody detects endogenous levels of total BCL2L14.
RRID:
AB_2842416
Cite Format: Affinity Biosciences Cat# DF9220, RRID:AB_2842416.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Apoptosis facilitator Bcl 2 like protein 14; Apoptosis regulator BCL G; Bcl2 L 14; BCL2 like 14 (apoptosis facilitator); BCL2 like 14; BCL2L14; BCLG; testicular tissue protein Li 26;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9BZR8 B2L14_HUMAN:

Isoform 1 is widely expressed. Isoform 2 is testis-specific.

Sequence:
MCSTSGCDLEEIPLDDDDLNTIEFKILAYYTRHHVFKSTPALFSPKLLRTRSLSQRGLGNCSANESWTEVSWPCRNSQSSEKAINLGKKKSSWKAFFGVVEKEDSQSTPAKVSAQGQRTLEYQDSHSQQWSRCLSNVEQCLEHEAVDPKVISIANRVAEIVYSWPPPQATQAGGFKSKEIFVTEGLSFQLQGHVPVASSSKKDEEEQILAKIVELLKYSGDQLERKLKKDKALMGHFQDGLSYSVFKTITDQVLMGVDPRGESEVKAQGFKAALVIDVTAKLTAIDNHPMNRVLGFGTKYLKENFSPWIQQHGGWEKILGISHEEVD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
83
Sheep
83
Rabbit
71
Pig
67
Horse
55
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BZR8 As Substrate

Site PTM Type Enzyme
S38 Phosphorylation
S44 Phosphorylation
S52 Phosphorylation
S125 Phosphorylation
S135 Phosphorylation
S152 Phosphorylation

Research Backgrounds

Function:

Plays a role in apoptosis.

PTMs:

Phosphorylated by MELK, leading to inhibit its pro-apoptotic function.

Subcellular Location:

Cytoplasm.

Cytoplasm>Cytosol.
Note: Diffusely distributed throughout the cytosol.

Endomembrane system.
Note: Predominantly localized to cytosolic organelles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is widely expressed. Isoform 2 is testis-specific.

Family&Domains:

Belongs to the Bcl-2 family.

Restrictive clause

 

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For Research Use Only.
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