AQP9 Antibody - #DF9225

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Product: AQP9 Antibody
Catalog: DF9225
Description: Rabbit polyclonal antibody to AQP9
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 31 kDa; 31kD(Calculated).
Uniprot: O43315
RRID: AB_2842421

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(83%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
AQP9 Antibody detects endogenous levels of total AQP9.
RRID:
AB_2842421
Cite Format: Affinity Biosciences Cat# DF9225, RRID:AB_2842421.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AQP 9; AQP-9; Aqp9; AQP9_HUMAN; Aquaglyceroporin-9; Aquaporin-9; Aquaporin9; HsT 17287; HsT17287; Small solute channel 1; SSC 1; SSC1;

Immunogens

Immunogen:
Uniprot:

O43315(AQP9_HUMAN) >>Visit HPA database.

Gene(ID):
AQP9(366)
Expression:
O43315 AQP9_HUMAN:

Highly expressed in peripheral leukocytes. Also expressed in liver, lung, and spleen.

Sequence:
MQPEGAEKGKSFKQRLVLKSSLAKETLSEFLGTFILIVLGCGCVAQAILSRGRFGGVITINVGFSMAVAMAIYVAGGVSGGHINPAVSLAMCLFGRMKWFKLPFYVGAQFLGAFVGAATVFGIYYDGLMSFAGGKLLIVGENATAHIFATYPAPYLSLANAFADQVVATMILLIIVFAIFDSRNLGAPRGLEPIAIGLLIIVIASSLGLNSGCAMNPARDLSPRLFTALAGWGFEVFRAGNNFWWIPVVGPLVGAVIGGLIYVLVIEIHHPEPDSVFKTEQSEDKPEKYELSVIM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
92
Horse
83
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43315 As Substrate

Site PTM Type Enzyme
S11 Phosphorylation Q05513 (PRKCZ)
S222 Phosphorylation Q05513 (PRKCZ)

Research Backgrounds

Function:

Forms a water channel with a broad specificity. Also permeable glycerol and urea. Mediates passage of a wide variety of small, non-charged solutes including carbamides, polyols, purines, and pyrimidines.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in peripheral leukocytes. Also expressed in liver, lung, and spleen.

Family&Domains:

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Belongs to the MIP/aquaporin (TC 1.A.8) family.

References

1). LncRNA-mRNA co-expression analysis reveals aquaporin-9-promoted neutrophil extracellular trap formation and inflammatory activation in sepsis. International immunopharmacology, 2024 (PubMed: 39133961) [IF=4.8]
2). Tanshinone IIA increased amniotic fluid volume through down-regulating placental AQPs expression via inhibiting the activity of GSK-3β. CELL AND TISSUE RESEARCH, 2022 (PubMed: 35674921) [IF=3.2]
3). Arctiin alleviates functional constipation by enhancing intestinal motility in mice. Experimental and Therapeutic Medicine, 2023 (PubMed: 37090075) [IF=2.4]

Application: IF/ICC    Species: Mouse    Sample: colon tissue

Figure 5 Arc reverses loperamide-induced elevation of AQP-3 and AQP-9. Immunofluorescence analysis of (A) AQP-3 and (B) AQP-9 in colon tissue. (C) Evaluation of immunofluorescent staining was performed using Image-Pro Plus software. (D) Western blot analysis of protein expression levels of AQP-3 and AQP-9. (E) Densitometry analysis of western blots. **P

Application: WB    Species: Mouse    Sample: colon tissue

Figure 5 Arc reverses loperamide-induced elevation of AQP-3 and AQP-9. Immunofluorescence analysis of (A) AQP-3 and (B) AQP-9 in colon tissue. (C) Evaluation of immunofluorescent staining was performed using Image-Pro Plus software. (D) Western blot analysis of protein expression levels of AQP-3 and AQP-9. (E) Densitometry analysis of western blots. **P

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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