ATE1 Antibody | Affinity Biosciences

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ATE1 Antibody - Western blot analysis of ATE1 using COLO205 whole cell lysates.

Product:	ATE1 Antibody
Catalog:	DF9226
Description:	Rabbit polyclonal antibody to ATE1
Application:	WB
Reactivity:	Human
Prediction:	Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	59 kDa; 59kD(Calculated).
Uniprot:	O95260
RRID:	AB_2842422

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

ATE1 Antibody detects endogenous levels of total ATE1.

RRID:

AB_2842422
Cite Format: Affinity Biosciences Cat# DF9226, RRID:AB_2842422.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AI225793; Arginine tRNA protein transferase 1; Arginine-tRNA--protein transferase 1; Arginyl-tRNA--protein transferase 1; Arginyl-tRNA-protein transferase; Arginyltransferase 1; Ate1; ATE1_HUMAN; AW547406; EC 2.3.2.8; MGC26724; OTTHUMP00000020637; OTTHUMP00000058665; R transferase 1; R-transferase 1;

Immunogens

Immunogen:

Uniprot:

O95260(ATE1_HUMAN) >>Visit HPA database.

Gene(ID):

ATE1(11101)

Sequence:

O95260 ATE1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAFWAGGSPSVVDYFPSEDFYRCGYCKNESGSRSNGMWAHSMTVQDYQDLIDRGWRRSGKYVYKPVMNQTCCPQYTIRCRPLQFQPSKSHKKVLKKMLKFLAKGEVPKGSCEDEPMDSTMDDAVAGDFALINKLDIQCDLKTLSDDIKESLESEGKNSKKEEPQELLQSQDFVGEKLGSGEPSHSVKVHTVPKPGKGADLSKPPCRKAKEIRKERKRLKLMQQNPAGELEGFQAQGHPPSLFPPKAKSNQPKSLEDLIFESLPENASHKLEVRVVRSSPPSSQFKATLLESYQVYKRYQMVIHKNPPDTPTESQFTRFLCSSPLEAETPPNGPDCGYGSFHQQYWLDGKIIAVGVIDILPNCVSSVYLYYDPDYSFLSLGVYSALREIAFTRQLHEKTSQLSYYYMGFYIHSCPKMKYKGQYRPSDLLCPETYVWVPIEQCLPSLENSKYCRFNQDPEAVDEDRSTEPDRLQVFHKRAIMPYGVYKKQQKDPSEEAAVLQYASLVGQKCSERMLLFRN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Sheep

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95260 As Substrate

O95260

Site	PTM Type	Source
Y61	Phosphorylation	Uniprot
Y63	Phosphorylation	Uniprot
Y75	Phosphorylation	Uniprot
K103	Ubiquitination	Uniprot
S110	Phosphorylation	Uniprot
K141	Ubiquitination	Uniprot
K148	Ubiquitination	Uniprot
K160	Ubiquitination	Uniprot
S169	Phosphorylation	Uniprot
K176	Ubiquitination	Uniprot
S179	Phosphorylation	Uniprot
S240	Phosphorylation	Uniprot
K252	Ubiquitination	Uniprot
K269	Ubiquitination	Uniprot
K285	Methylation	Uniprot
S291	Phosphorylation	Uniprot
Y292	Phosphorylation	Uniprot
Y422	Phosphorylation	Uniprot
Y433	Phosphorylation	Uniprot
R464	Methylation	Uniprot
Y482	Phosphorylation	Uniprot
K490	Ubiquitination	Uniprot
Y501	Phosphorylation	Uniprot
K508	Ubiquitination	Uniprot

Research Backgrounds

O95260_ATE1_HUMAN

Function:

Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway. Does not arginylate cysteine residues (By similarity).

Subcellular Location:

Nucleus. Cytoplasm.

Cytoplasm.

Subunit Structure:

Monomer. Interacts with LIAT1 (By similarity).

Family&Domains:

Belongs to the R-transferase family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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ATE1 Antibody - #DF9226