Product: FOX1 Antibody
Catalog: DF9232
Description: Rabbit polyclonal antibody to FOX1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 43 kDa,55kDa; 43kD(Calculated).
Uniprot: Q9NWB1
RRID: AB_2842428

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
FOX1 Antibody detects endogenous levels of total FOX1.
RRID:
AB_2842428
Cite Format: Affinity Biosciences Cat# DF9232, RRID:AB_2842428.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2BP1; A2 BP1; A2BP 1; A2BP; A2BP1; Ataxin 2 binding protein 1; Ataxin-2-binding protein 1; Ataxin2 binding protein 1; FOX 1; Fox-1 homolog A; fox-1-like RNA-binding protein 1; Hexaribonucleotide binding protein 1; Hexaribonucleotide-binding protein 1; HRNBP 1; HRNBP1; RBFOX1; RFOX1_HUMAN; RNA binding protein fox-1 homolog 1; RNA binding protein fox-1 homolog 1, C. elegans, homolog of, 1; RNA binding protein, fox-1 homolog (C. elegans) 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9NWB1 RFOX1_HUMAN:

Predominantly expressed in muscle and brain.

Sequence:
MNCEREQLRGNQEAAAAPDTMAQPYASAQFAPPQNGIPAEYTAPHPHPAPEYTGQTTVPEHTLNLYPPAQTHSEQSPADTSAQTVSGTATQTDDAAPTDGQPQTQPSENTENKSQPKRLHVSNIPFRFRDPDLRQMFGQFGKILDVEIIFNERGSKGFGFVTFENSADADRAREKLHGTVVEGRKIEVNNATARVMTNKKTVNPYTNGWKLNPVVGAVYSPEFYAGTVLLCQANQEGSSMYSAPSSLVYTSAMPGFPYPAATAAAAYRGAHLRGRGRTVYNTFRAAAPPPPIPAYGGVVYQDGFYGADIYGGYAAYRYAQPTPATAAAYSDSYGRVYAADPYHHALAPAPTYGVGAMNAFAPLTDAKTRSHADDVGLVLSSLQASIYRGGYNRFAPY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
92
Zebrafish
70
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NWB1 As Substrate

Site PTM Type Enzyme
R134 Methylation
K156 Ubiquitination
K175 Acetylation
K175 Ubiquitination
K185 Ubiquitination
Y205 Phosphorylation

Research Backgrounds

Function:

RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.

Subcellular Location:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Predominantly expressed in muscle and brain.

Subunit Structure:

Binds to the C-terminus of ATXN2.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.