CLCN2 Antibody - #DF9375
| Product: | CLCN2 Antibody |
| Catalog: | DF9375 |
| Description: | Rabbit polyclonal antibody to CLCN2 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 98 kDa; 99kD(Calculated). |
| Uniprot: | P51788 |
| RRID: | AB_2842571 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9375, RRID:AB_2842571.
Fold/Unfold
Chloride Channel 2; Chloride channel protein 2; Chloride channel, voltage sensitive 2; CIC 2; CIC2; ClC-2; CLC2; Clcn2; CLCN2_HUMAN; ECA2; ECA3; EG13; EGI11; EGMA; EJM6; EJM8; PKA-activated chloride channel;
Immunogens
Ubiquitously expressed. Moderately expressed in aortic and coronary vascular smooth muscle cells and expressed at a low level in aortic endothelial cells. Expressed in the adrenal gland, predominantly in the zona glomerulosa (PubMed:29403011).
- P51788 CLCN2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAAAEEGMEPRALQYEQTLMYGRYTQDLGAFAKEEAARIRLGGPEPWKGPPSSRAAPELLEYGRSRCARCRVCSVRCHKFLVSRVGEDWIFLVLLGLLMALVSWVMDYAIAACLQAQQWMSRGLNTSILLQYLAWVTYPVVLITFSAGFTQILAPQAVGSGIPEMKTILRGVVLKEYLTLKTFIAKVIGLTCALGSGMPLGKEGPFVHIASMCAALLSKFLSLFGGIYENESRNTEMLAAACAVGVGCCFAAPIGGVLFSIEVTSTFFAVRNYWRGFFAATFSAFIFRVLAVWNRDEETITALFKTRFRLDFPFDLQELPAFAVIGIASGFGGALFVYLNRKIVQVMRKQKTINRFLMRKRLLFPALVTLLISTLTFPPGFGQFMAGQLSQKETLVTLFDNRTWVRQGLVEELEPPSTSQAWNPPRANVFLTLVIFILMKFWMSALATTIPVPCGAFMPVFVIGAAFGRLVGESMAAWFPDGIHTDSSTYRIVPGGYAVVGAAALAGAVTHTVSTAVIVFELTGQIAHILPVMIAVILANAVAQSLQPSLYDSIIRIKKLPYLPELGWGRHQQYRVRVEDIMVRDVPHVALSCTFRDLRLALHRTKGRMLALVESPESMILLGSIERSQVVALLGAQLSPARRRQHMQERRATQTSPLSDQEGPPTPEASVCFQVNTEDSAFPAARGETHKPLKPALKRGPSVTRNLGESPTGSAESAGIALRSLFCGSPPPEAASEKLESCEKRKLKRVRISLASDADLEGEMSPEEILEWEEQQLDEPVNFSDCKIDPAPFQLVERTSLHKTHTIFSLLGVDHAYVTSIGRLIGIVTLKELRKAIEGSVTAQGVKVRPPLASFRDSATSSSDTETTEVHALWGPHSRHGLPREGSPSDSDDKCQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P51788 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T20 | Phosphorylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| T193 | Phosphorylation | Uniprot | |
| T308 | Phosphorylation | Uniprot | |
| T607 | Phosphorylation | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| S641 | Phosphorylation | Uniprot | |
| K693 | Acetylation | Uniprot | |
| K696 | Acetylation | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| S712 | Phosphorylation | Uniprot | |
| S716 | Phosphorylation | Uniprot | |
| S719 | Phosphorylation | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| S731 | Phosphorylation | Uniprot | |
| S738 | Phosphorylation | Uniprot | |
| S743 | Phosphorylation | Uniprot | |
| T801 | Phosphorylation | Uniprot | |
| S802 | Phosphorylation | Uniprot | |
| S856 | Phosphorylation | Uniprot |
Research Backgrounds
Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport. Involved in the regulation of aldosterone production. The opening of CLCN2 channels at hyperpolarized membrane potentials in the glomerulosa causes cell membrane depolarization, activation of voltage-gated Ca2+ channels and increased expression of aldosterone synthase, the rate-limiting enzyme for aldosterone biosynthesis.
Phosphorylated. Activated by dephosphorylation.
Cell membrane>Multi-pass membrane protein.
Ubiquitously expressed. Moderately expressed in aortic and coronary vascular smooth muscle cells and expressed at a low level in aortic endothelial cells. Expressed in the adrenal gland, predominantly in the zona glomerulosa.
Belongs to the chloride channel (TC 2.A.49) family. ClC-2/CLCN2 subfamily.
Research Fields
· Organismal Systems > Digestive system > Mineral absorption.
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