Claudin 14 Antibody - #DF9391

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Product: Claudin 14 Antibody
Catalog: DF9391
Description: Rabbit polyclonal antibody to Claudin 14
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 26 kDa; 26kD(Calculated).
Uniprot: O95500
RRID: AB_2842587

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(80%), Bovine(90%), Horse(90%), Rabbit(100%), Dog(80%), Chicken(90%)
Clonality:
Polyclonal
Specificity:
Claudin 14 Antibody detects endogenous levels of total Claudin 14.
RRID:
AB_2842587
Cite Format: Affinity Biosciences Cat# DF9391, RRID:AB_2842587.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI851731; Claudin 14; Claudin-14; CLD14_HUMAN; CLDN 14; CLDN14; DFNB 29; DFNB29; Human CLDN14 gene; MGC109472; OTTHUMP00000109046; OTTHUMP00000109049; OTTMUSP00000021531; UNQ777/PRO1571;

Immunogens

Immunogen:
Uniprot:

O95500(CLD14_HUMAN) >>Visit HPA database.

Gene(ID):
CLDN14(23562)
Expression:
O95500 CLD14_HUMAN:

Liver, kidney. Also found in ear.

Sequence:
MASTAVQLLGFLLSFLGMVGTLITTILPHWRRTAHVGTNILTAVSYLKGLWMECVWHSTGIYQCQIYRSLLALPQDLQAARALMVISCLLSGIACACAVIGMKCTRCAKGTPAKTTFAILGGTLFILAGLLCMVAVSWTTNDVVQNFYNPLLPSGMKFEIGQALYLGFISSSLSLIGGTLLCLSCQDEAPYRPYQAPPRATTTTANTAPAYQPPAAYKDNRAPSVTSATHSGYRLNDYV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
90
Bovine
90
Chicken
90
Pig
80
Dog
80
Xenopus
70
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95500 As Substrate

Site PTM Type Enzyme
Y194 Phosphorylation
Y211 Phosphorylation
Y217 Phosphorylation
Y233 Phosphorylation

Research Backgrounds

Function:

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

Subcellular Location:

Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Liver, kidney. Also found in ear.

Family&Domains:

Belongs to the claudin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

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