TUBGCP2 Antibody - #DF9527

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Product: TUBGCP2 Antibody
Catalog: DF9527
Description: Rabbit polyclonal antibody to TUBGCP2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 103 kDa; 103kD(Calculated).
Uniprot: Q9BSJ2
RRID: AB_2842723

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(82%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
TUBGCP2 Antibody detects endogenous levels of total TUBGCP2.
RRID:
AB_2842723
Cite Format: Affinity Biosciences Cat# DF9527, RRID:AB_2842723.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1700022B05Rik; A disintegrin and metalloprotease domain 8; Adam8; Gamma ring complex protein 103 kDa; Gamma tubulin complex component 2; Gamma tubulin complex protein 2; Gamma-ring complex protein 103 kDa; Gamma-tubulin complex component 2; GCP 2; GCP-2; GCP2; GCP2_HUMAN; h103p; hGCP2; hGrip103; hSpc97; MGC138162; MGC83533; OTTHUMP00000020808; SPBC97; Spc97p; Spindle pole body protein Spc97 homolog; TUBGCP 2; Tubgcp2; Tubulin gamma complex associated protein 2;

Immunogens

Immunogen:
Uniprot:

Q9BSJ2(GCP2_HUMAN) >>Visit HPA database.

Gene(ID):
TUBGCP2(10844)
Expression:
Q9BSJ2 GCP2_HUMAN:

Ubiquitously expressed.

Sequence:
MSEFRIHHDVNELLSLLRVHGGDGAEVYIDLLQKNRTPYVTTTVSAHSAKVKIAEFSRTPEDFLKKYDELKSKNTRNLDPLVYLLSKLTEDKETLQYLQQNAKERAELAAAAVGSSTTSINVPAAASKISMQELEELRKQLGSVATGSTLQQSLELKRKMLRDKQNKKNSGQHLPIFPAWVYERPALIGDFLIGAGISTDTALPIGTLPLASQESAVVEDLLYVLVGVDGRYVSAQPLAGRQSRTFLVDPNLDLSIRELVHRILPVAASYSAVTRFIEEKSSFEYGQVNHALAAAMRTLVKEHLILVSQLEQLHRQGLLSLQKLWFYIQPAMRTMDILASLATSVDKGECLGGSTLSLLHDRSFSYTGDSQAQELCLYLTKAASAPYFEVLEKWIYRGIIHDPYSEFMVEEHELRKERIQEDYNDKYWDQRYTIVQQQIPSFLQKMADKILSTGKYLNVVRECGHDVTCPVAKEIIYTLKERAYVEQIEKAFNYASKVLLDFLMEEKELVAHLRSIKRYFLMDQGDFFVHFMDLAEEELRKPVEDITPPRLEALLELALRMSTANTDPFKDDLKIDLMPHDLITQLLRVLAIETKQEKAMAHADPTELALSGLEAFSFDYIVKWPLSLIINRKALTRYQMLFRHMFYCKHVERQLCSVWISNKTAKQHSLHSAQWFAGAFTLRQRMLNFVQNIQYYMMFEVMEPTWHILEKNLKSASNIDDVLGHHTGFLDTCLKDCMLTNPELLKVFSKLMSVCVMFTNCMQKFTQSMKLDGELGGQTLEHSTVLGLPAGAEERARKELARKHLAEHADTVQLVSGFEATINKFDKNFSAHLLDLLARLSIYSTSDCEHGMASVISRLDFNGFYTERLERLSAERSQKATPQVPVLRGPPAPAPRVAVTAQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Sheep
82
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BSJ2 As Substrate

Site PTM Type Enzyme
T37 Phosphorylation
Y39 Phosphorylation
K65 Ubiquitination
Y83 Phosphorylation
S86 Phosphorylation
K92 Ubiquitination
K103 Ubiquitination
K157 Ubiquitination
K426 Ubiquitination
K449 Ubiquitination
K455 Ubiquitination
T468 Phosphorylation
K480 Ubiquitination
K490 Ubiquitination
T584 Phosphorylation
T594 Phosphorylation
K595 Ubiquitination
K714 Ubiquitination
K735 Ubiquitination
K803 Ubiquitination
K827 Acetylation
S873 Phosphorylation
K879 Ubiquitination
T881 Phosphorylation

Research Backgrounds

Function:

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Gamma-tubulin complex is composed of gamma-tubulin, TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6. Interacts with ATF5; the ATF5:PCNT:polyglutamylated tubulin (PGT) tripartite unites the mother centriole and the pericentriolar material (PCM) in the centrosome.

Family&Domains:

Belongs to the TUBGCP family.

Restrictive clause

 

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