SERP1 Antibody | Affinity Biosciences

IHC
IF/ICC
Cites

1/3

DF9873 at 1/100 staining Mouse colorectal tissue by IHC-P.

Product:	SERP1 Antibody
Catalog:	DF9873
Description:	Rabbit polyclonal antibody to SERP1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Rabbit, Chicken
Mol.Wt.:	7 kDa; 7kD(Calculated).
Uniprot:	Q9Y6X1
RRID:	AB_2843067

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Rabbit(100%), Chicken(92%)

Clonality:

Polyclonal

Specificity:

SERP1 Antibody detects endogenous levels of total SERP1.

RRID:

AB_2843067
Cite Format: Affinity Biosciences Cat# DF9873, RRID:AB_2843067.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

D3Ucla1; Ramp4; Ribosome associated membrane protein 4; Ribosome attached membrane protein 4; Ribosome-attached membrane protein 4; Serp1; SERP1_HUMAN; Stress associated endoplasmic reticulum protein 1; Stress-associated endoplasmic reticulum protein 1;

Immunogens

Immunogen:

Uniprot:

Q9Y6X1(SERP1_HUMAN) >>Visit HPA database.

Gene(ID):

SERP1(27230)

Sequence:

Q9Y6X1 SERP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVAKQRIRMANEKHSKNITQRGNVAKTSRNAPEEKASVGPWLLALFIFVVCGSAIFQIIQSIRMGM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Horse

Sheep

Dog

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6X1 As Substrate

Q9Y6X1

Site	PTM Type	Source
K16	Ubiquitination	Uniprot
R21	Methylation	Uniprot
K26	Ubiquitination	Uniprot
S37	Phosphorylation	Uniprot

Research Backgrounds

Q9Y6X1_SERP1_HUMAN

Function:

Interacts with target proteins during their translocation into the lumen of the endoplasmic reticulum. Protects unfolded target proteins against degradation during ER stress. May facilitate glycosylation of target proteins after termination of ER stress. May modulate the use of N-glycosylation sites on target proteins (By similarity).

Subcellular Location:

Membrane>Single-pass type IV membrane protein. Endoplasmic reticulum membrane>Single-pass membrane protein>Cytoplasmic side.

Subunit Structure:

Interacts with SEC61B, SEC61A1 and the SEC61 complex. Interacts with CANX (By similarity).

Family&Domains:

Belongs to the RAMP4 family.

References

1). SERP1 reduces inchoate acute hepatic injury through regulation of endoplasmic reticulum stress via the GSK3β/β‑catenin/TCF/LEF signaling pathway. Molecular Medicine Reports (PubMed: 35419615) [IF=3.4]

Application: WB Species: Mouse Sample:

Figure 1. - Establishment of an inchoate acute hepatic injury model and detection of ERS-related proteins. (A) Pathological changes of tissues of mice in the model group detected by H&E staining. (B) Levels of alanine aminotransferase and aspartate aminotransferase in the serum. (C) ERS-related protein expression detected by western blotting. SERP1 expression in acute hepatic injury tissues detected by (D) immunohistochemistry and (E) western blotting. (F) SERP1 expression in acute hepatic injury cells detected by western blotting and reverse transcription-quantitative PCR. *P

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

SERP1 Antibody - #DF9873