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$280 100ul
$350 200ul

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  • Product Name
    STX6 Antibody
  • Catalog No.
    DF9956
  • RRID
    AB_2843150
  • Source
    Rabbit
  • Application
    WB,IHC,IF/ICC,ELISA(peptide)
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
  • UniProt
  • Mol.Wt
    29 kD;
    29kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

STX 6; STX6; STX-6; STX6_HUMAN; Syntaxin-6; Syntaxin6;

Applications:

WB 1:1000-3000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Specificity:

STX6 Antibody detects endogenous levels of total STX6.

RRID:

AB_2843150
Please cite this product as: Affinity Biosciences Cat# DF9956, RRID:AB_2843150.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human STX6, corresponding to a region within the internal amino acids.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

STX6

Molecular Weight:

Observed Mol.Wt.: 29 kD.
Predicted Mol.Wt.: 29kDa(Calculated)..

Sequence:
MSMEDPFFVVKGEVQKAVNTAQGLFQRWTELLQDPSTATREEIDWTTNELRNNLRSIEWDLEDLDETISIVEANPRKFNLDATELSIRKAFITSTRQVVRDMKDQMSTSSVQALAERKNRQALLGDSGSQNWSTGTTDKYGRLDRELQRANSHFIEEQQAQQQLIVEQQDEQLELVSGSIGVLKNMSQRIGGELEEQAVMLEDFSHELESTQSRLDNVMKKLAKVSHMTSDRRQWCAIAILFAVLLVVLILFLVL

Research Background

Function:

Involved in intracellular vesicle trafficking.

Subcellular Location:

Golgi apparatus membrane>Single-pass type IV membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Subunit Structure:

Identified in a complex containing STX6, STX12, VAMP4 and VTI1A. Binds EEA1 (By similarity). Interacts with VPS45A (By similarity). Interacts with MARCHF2 and MARCHF3 (By similarity). Interacts with GOPC. Interacts with UHRF1BP1L (via C-terminal coiled-coil domain). Interacts with BAIAP3; this interaction is increased in the presence of calcium.

Similarity:

Belongs to the syntaxin family.

Research Fields

Research Fields:

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

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Catalog Number :

DF9956-BP
(Blocking peptide available as DF9956-BP)

Price/Size :

$350/1mg.
Tips: For modified antibodies, we provide modified peptides(0.5mg) and non-modified peptides(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Horse
100%
Bovine
100%
Sheep
100%
Dog
100%
Chicken
100%
Rabbit
100%
Xenopus
83%
Zebrafish
83%
High similarity Medium similarity Low similarity No similarity
O43752 as Substrate
Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K77 Ubiquitination
T83 Phosphorylation
S86 Phosphorylation
K89 Ubiquitination
S107 Phosphorylation
K118 Ubiquitination
S127 Phosphorylation
S129 Phosphorylation
T134 Phosphorylation
T137 Phosphorylation
K139 Ubiquitination
Y140 Phosphorylation
S152 Phosphorylation
K184 Ubiquitination
T229 Phosphorylation
S230 Phosphorylation
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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