Product: ARP-1 Antibody
Catalog: AF9015
Description: Rabbit polyclonal antibody to ARP-1
Application: WB IF/ICC
Reactivity: Human
Prediction: Bovine, Dog
Mol.Wt.: 45kDa; 46kD(Calculated).
Uniprot: P24468
RRID: AB_2843206

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Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ARP-1 Antibody detects endogenous levels of total ARP-1.
RRID:
AB_2843206
Cite Format: Affinity Biosciences Cat# AF9015, RRID:AB_2843206.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Apolipoprotein A-I regulatory protein 1; Apolipoprotein AI regulatory protein 1; ARP-1; ARP1; COT2_HUMAN; COUP TF2; COUP transcription factor 2; COUP transcription factor II; COUP-TF II; COUP-TF2; NR2F2; Nuclear receptor subfamily 2 group F member 2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P24468 COT2_HUMAN:

Ubiquitous.

Sequence:
MAMVVSTWRDPQDEVPGSQGSQASQAPPVPGPPPGAPHTPQTPGQGGPASTPAQTAAGGQGGPGGPGSDKQQQQQHIECVVCGDKSSGKHYGQFTCEGCKSFFKRSVRRNLSYTCRANRNCPIDQHHRNQCQYCRLKKCLKVGMRREAVQRGRMPPTQPTHGQFALTNGDPLNCHSYLSGYISLLLRAEPYPTSRFGSQCMQPNNIMGIENICELAARMLFSAVEWARNIPFFPDLQITDQVALLRLTWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSADRVVAFMDHIRIFQEQVEKLKALHVDSAEYSCLKAIVLFTSDACGLSDVAHVESLQEKSQCALEEYVRSQYPNQPTRFGKLLLRLPSLRTVSSSVIEQLFFVRLVGKTPIETLIRDMLLSGSSFNWPYMAIQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
100
Chicken
75
Xenopus
70
Pig
0
Horse
0
Sheep
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P24468 As Substrate

Site PTM Type Enzyme
T39 Phosphorylation
T51 Phosphorylation
K89 Ubiquitination
S112 Phosphorylation
K141 Methylation
K301 Ubiquitination
Y348 Phosphorylation
K362 Ubiquitination
K389 Ubiquitination
T394 Phosphorylation

Research Backgrounds

Function:

Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with SQSTM1 (By similarity). Binds DNA as a dimer; homodimer or heterodimer with NR2F6. Interacts with NCOA1, NCOA2, NCOA3 and PPARGC1A. Interacts with ZFPM2 (By similarity).

Family&Domains:

Belongs to the nuclear hormone receptor family. NR2 subfamily.

Restrictive clause

 

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For Research Use Only.
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