CaMKIbeta Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of CaMKIbeta using LOVO whole cell lysates.

Product:	CaMKIbeta Antibody
Catalog:	AF9031
Description:	Rabbit polyclonal antibody to CaMKIbeta
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	38kDa; 39kD(Calculated).
Uniprot:	Q6P2M8
RRID:	AB_2843222

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

CaMKIbeta Antibody detects endogenous levels of total CaMKIbeta.

RRID:

AB_2843222
Cite Format: Affinity Biosciences Cat# AF9031, RRID:AB_2843222.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Calcium/calmodulin dependent protein kinase type 1B; Calcium/calmodulin-dependent protein kinase type 1B; CaM KI beta; CaM kinase I beta; CaM kinase IB; CaM-KI beta; CaMK1b; CaMKI beta; CaMKI-beta; EC 2.7.11.17; KCC1B_HUMAN; MGC45419; PNCK; Pregnancy up regulated non ubiquitously expressed CaM kinase; Pregnancy up-regulated non-ubiquitously-expressed CaM kinase;

Immunogens

Immunogen:

Uniprot:

Q6P2M8(KCC1B_HUMAN) >>Visit HPA database.

Gene(ID):

PNCK(139728)

Sequence:

Q6P2M8 KCC1B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLLLKKHTEDISSVYEIRERLGSGAFSEVVLAQERGSAHLVALKCIPKKALRGKEALVENEIAVLRRISHPNIVALEDVHESPSHLYLAMELVTGGELFDRIMERGSYTEKDASHLVGQVLGAVSYLHSLGIVHRDLKPENLLYATPFEDSKIMVSDFGLSKIQAGNMLGTACGTPGYVAPELLEQKPYGKAVDVWALGVISYILLCGYPPFYDESDPELFSQILRASYEFDSPFWDDISESAKDFIRHLLERDPQKRFTCQQALRHLWISGDTAFDRDILGSVSEQIRKNFARTHWKRAFNATSFLRHIRKLGQIPEGEGASEQGMARHSHSGLRAGQPPKW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Horse

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6P2M8 As Substrate

Q6P2M8

Site	PTM Type	Enzyme	Source
T171	Phosphorylation		Uniprot
Y229	Phosphorylation		Uniprot

PTMs - Q6P2M8 As Enzyme

Q6P2M8

Substrate	Site	Source
O43432 (EIF4G3)	S1156	Uniprot
P07900 (HSP90AA1)	S391	Uniprot
P07900 (HSP90AA1)	T624	Uniprot
P08238 (HSP90AB1)	T89	Uniprot

Research Backgrounds

Q6P2M8_KCC1B_HUMAN

Function:

Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1 (By similarity).

PTMs:

Phosphorylated by CAMKK1.

Subcellular Location:

Cytoplasm. Nucleus.

Family&Domains:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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CaMKIbeta Antibody - #AF9031