Product: Casein Kinase Igamma2 Antibody
Catalog: AF9034
Description: Rabbit polyclonal antibody to Casein Kinase Igamma2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 47kDa; 47kD(Calculated).
Uniprot: P78368
RRID: AB_2843225

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(92%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
Casein Kinase Igamma2 Antibody detects endogenous levels of total Casein Kinase Igamma2.
RRID:
AB_2843225
Cite Format: Affinity Biosciences Cat# AF9034, RRID:AB_2843225.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Casein kinase 1 gamma 2; Casein kinase I isoform gamma 1; Casein kinase I isoform gamma 3; Casein kinase I isoform gamma-2; CK1g2; CKI gamma 1; CKI gamma 2; CKI gamma 3; CSNK1G1; CSNK1G2; CSNK1G3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
MDFDKKGGKGETEEGRRMSKAGGGRSSHGIRSSGTSSGVLMVGPNFRVGKKIGCGNFGELRLGKNLYTNEYVAIKLEPIKSRAPQLHLEYRFYKQLSATEGVPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFTLKTVLMIAIQLITRMEYVHTKSLIYRDVKPENFLVGRPGTKRQHAIHIIDFGLAKEYIDPETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRATPIEVLCENFPEEMATYLRYVRRLDFFEKPDYDYLRKLFTDLFDRSGFVFDYEYDWAGKPLPTPIGTVHTDLPSQPQLRDKTQPHSKNQALNSTNGELNADDPTAGHSNAPITAPAEVEVADETKCCCFFKRRKRKSLQRHK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
92
Chicken
92
Horse
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P78368 As Substrate

Site PTM Type Enzyme
R25 Methylation
S26 Phosphorylation
S32 Phosphorylation
S33 Phosphorylation
T35 Phosphorylation
S36 Phosphorylation
K51 Ubiquitination
K64 Ubiquitination
Y67 Phosphorylation
T68 Phosphorylation
Y71 Phosphorylation
Y90 Phosphorylation
Y93 Phosphorylation
T136 Phosphorylation
T138 Phosphorylation
T141 Phosphorylation
T151 Phosphorylation
T158 Phosphorylation
K167 Ubiquitination
Y195 Phosphorylation
K256 Ubiquitination
T259 Phosphorylation
K266 Ubiquitination
K310 Ubiquitination
T336 Phosphorylation
S359 Phosphorylation
S366 Phosphorylation
T367 Phosphorylation
T377 Phosphorylation
S381 Phosphorylation
K404 Ubiquitination
S410 Phosphorylation

PTMs - P78368 As Enzyme

Substrate Site Source
P84022 (SMAD3) S418 Uniprot
Q14457 (BECN1) T406 Uniprot
Q14457 (BECN1) S409 Uniprot
Q9Y5P4 (COL4A3BP) S132 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation.

PTMs:

Autophosphorylated.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Testis.

Subunit Structure:

Monomer (By similarity). Interacts with MTA1 (short isoform) in the cytoplasm. Interacts with SMAD3.

Family&Domains:

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway.   (View pathway)

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