CNOT2 Antibody - #AF9043

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Product: CNOT2 Antibody
Catalog: AF9043
Description: Rabbit polyclonal antibody to CNOT2
Application: WB
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 46kDa; 60kD(Calculated).
Uniprot: Q9NZN8
RRID: AB_2843234

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
CNOT2 Antibody detects endogenous levels of total CNOT2.
RRID:
AB_2843234
Cite Format: Affinity Biosciences Cat# AF9043, RRID:AB_2843234.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CCR4 associated factor 2; CCR4 NOT transcription complex subunit 2; CCR4-associated factor 2; CCR4-NOT transcription complex subunit 2; CDC36; CNOT2; CNOT2_HUMAN; HSPC131; MSTP046; Negative regulator of transcription 2; NOT2 (negative regulator of transcription 2 yeast) homolog; NOT2; NOT2H;

Immunogens

Immunogen:
Uniprot:

Q9NZN8(CNOT2_HUMAN) >>Visit HPA database.

Gene(ID):
CNOT2(4848)
Expression:
Q9NZN8 CNOT2_HUMAN:

Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocytes.

Sequence:
MVRTDGHTLSEKRNYQVTNSMFGASRKKFVEGVDSDYHDENMYYSQSSMFPHRSEKDMLASPSTSGQLSQFGASLYGQQSALGLPMRGMSNNTPQLNRSLSQGTQLPSHVTPTTGVPTMSLHTPPSPSRGILPMNPRNMMNHSQVGQGIGIPSRTNSMSSSGLGSPNRSSPSIICMPKQQPSRQPFTVNSMSGFGMNRNQAFGMNNSLSSNIFNGTDGSENVTGLDLSDFPALADRNRREGSGNPTPLINPLAGRAPYVGMVTKPANEQSQDFSIHNEDFPALPGSSYKDPTSSNDDSKSNLNTSGKTTSSTDGPKFPGDKSSTTQNNNQQKKGIQVLPDGRVTNIPQGMVTDQFGMIGLLTFIRAAETDPGMVHLALGSDLTTLGLNLNSPENLYPKFASPWASSPCRPQDIDFHVPSEYLTNIHIRDKLAAIKLGRYGEDLLFYLYYMNGGDVLQLLAAVELFNRDWRYHKEERVWITRAPGMEPTMKTNTYERGTYYFFDCLNWRKVAKEFHLEYDKLEERPHLPSTFNYNPAQQAF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
73
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NZN8 As Substrate

Site PTM Type Enzyme
K12 Ubiquitination
S35 Phosphorylation
Y37 Phosphorylation
Y43 Phosphorylation
Y44 Phosphorylation
S45 Phosphorylation
S47 Phosphorylation
S48 Phosphorylation
K56 Acetylation
S61 Phosphorylation
S63 Phosphorylation
S65 Phosphorylation
S69 Phosphorylation
R87 Methylation
S90 Phosphorylation
T93 Phosphorylation
S99 Phosphorylation
S101 Phosphorylation Q13535 (ATR)
T104 Phosphorylation
S108 Phosphorylation
T111 Phosphorylation
T113 Phosphorylation
T114 Phosphorylation
T118 Phosphorylation
S120 Phosphorylation
T123 Phosphorylation
S126 Phosphorylation
S128 Phosphorylation
R137 Methylation
S143 Phosphorylation
T155 Phosphorylation
S157 Phosphorylation
S159 Phosphorylation
S160 Phosphorylation
S161 Phosphorylation
S165 Phosphorylation
S169 Phosphorylation
S170 Phosphorylation
S242 Phosphorylation
T246 Phosphorylation
Y258 Phosphorylation
S274 Phosphorylation
S293 Phosphorylation
S294 Phosphorylation
K299 Acetylation
T304 Phosphorylation
S305 Phosphorylation
K307 Ubiquitination
T308 Phosphorylation
K321 Ubiquitination
S391 Phosphorylation
Y396 Phosphorylation
Y471 Phosphorylation
T480 Phosphorylation
K490 Ubiquitination
Y494 Phosphorylation
Y500 Phosphorylation
K512 Ubiquitination
K520 Ubiquitination

Research Backgrounds

Function:

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Required for the CCR4-NOT complex structural integrity. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may specifically involve the N-Cor repressor complex containing HDAC3, NCOR1 and NCOR2. Involved in the maintenance of embryonic stem (ES) cell identity.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocytes.

Subunit Structure:

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex interacts directly with CNOT3. Interacts with NCOR1, NCOR2. HDAC3 and GPS2.

Family&Domains:

Belongs to the CNOT2/3/5 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

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