GS28 Antibody - #AF9082

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Product: GS28 Antibody
Catalog: AF9082
Description: Rabbit polyclonal antibody to GS28
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 37kDa; 29kD(Calculated).
Uniprot: O95249
RRID: AB_2843273

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
GS28 Antibody detects endogenous levels of total GS28.
RRID:
AB_2843273
Cite Format: Affinity Biosciences Cat# AF9082, RRID:AB_2843273.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

28 kDa cis Golgi SNARE p28; 28 kDa Golgi SNARE protein; cis-golgi SNARE; golgi integral membrane protein 2; Golgi SNAP receptor complex member 1; Golgi SNARE 28 kDa; GOLIM2; GOS28; GOS28/P28; GOSR1; GS25; Gs28; P28;

Immunogens

Immunogen:
Uniprot:

O95249(GOSR1_HUMAN) >>Visit HPA database.

Gene(ID):
GOSR1(9527)
Sequence:
MAAGTSSYWEDLRKQARQLENELDLKLVSFSKLCTSYSHSSTRDGRRDRYSSDTTPLLNGSSQDRMFETMAIEIEQLLARLTGVNDKMAEYTNSAGVPSLNAALMHTLQRHRDILQDYTHEFHKTKANFMAIRERENLMGSVRKDIESYKSGSGVNNRRTELFLKEHDHLRNSDRLIEETISIAMATKENMTSQRGMLKSIHSKMNTLANRFPAVNSLIQRINLRKRRDSLILGGVIGICTILLLLYAFH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95249 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K26 Ubiquitination
K32 Ubiquitination
S36 Phosphorylation
Y37 Phosphorylation
S38 Phosphorylation
S40 Phosphorylation
S51 Phosphorylation
S52 Phosphorylation
K124 Acetylation
K124 Ubiquitination
S141 Phosphorylation
K144 Ubiquitination
S148 Phosphorylation
Y149 Phosphorylation
K150 Methylation
K150 Ubiquitination
T160 Phosphorylation
K165 Ubiquitination
K188 Ubiquitination
K199 Ubiquitination
K204 Ubiquitination
S217 Phosphorylation

Research Backgrounds

Function:

Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor. May play a protective role against hydrogen peroxide induced cytotoxicity under glutathione depleted conditions in neuronal cells by regulating the intracellular ROS levels via inhibition of p38 MAPK (MAPK11, MAPK12, MAPK13 and MAPK14). Participates in docking and fusion stage of ER to cis-Golgi transport. Plays an important physiological role in VLDL-transport vesicle-Golgi fusion and thus in VLDL delivery to the hepatic cis-Golgi.

Subcellular Location:

Golgi apparatus membrane>Single-pass type IV membrane protein.
Note: Localizes throughout the Golgi apparatus, with lowest levels in the trans-Golgi network (By similarity). Enriched on vesicular components at the terminal rims of the Golgi. Found in Golgi microtubules at low temperature (15 degrees Celsius).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of several multiprotein Golgi SNARE complexes. Identified in a SNARE complex with BET1, STX5 and YKT6, in a SNARE complex with BET1L, STX5 and YKT6, in a SNARE complex with STX5, GOSR2, SEC22B and BET1, and in complex with STX5 and COG3. Interacts with GABARAPL2 (By similarity).

Family&Domains:

Belongs to the GOSR1 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

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