MRCKbeta Antibody - #AF9116

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Product: MRCKbeta Antibody
Catalog: AF9116
Description: Rabbit polyclonal antibody to MRCKbeta
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog
Mol.Wt.: 194kDa; 194kD(Calculated).
Uniprot: Q9Y5S2
RRID: AB_2843306

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MRCKbeta Antibody detects endogenous levels of total MRCKbeta.
RRID:
AB_2843306
Cite Format: Affinity Biosciences Cat# AF9116, RRID:AB_2843306.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CDC42 binding protein kinase beta; CDC42-binding protein kinase beta; CDC42BPB; DMPK like beta; DMPK-like beta; KIAA1124; MRCK beta; MRCKB_HUMAN; Myotonic dystrophy kinase related CDC42 binding kinase beta; Myotonic dystrophy kinase-related CDC42-binding kinase beta; Myotonic dystrophy protein kinase like beta; Myotonic dystrophy protein kinase-like beta; Serine/threonine protein kinase MRCK beta; Serine/threonine-protein kinase MRCK beta;

Immunogens

Immunogen:
Uniprot:

Q9Y5S2(MRCKB_HUMAN) >>Visit HPA database.

Gene(ID):
CDC42BPB(9578)
Expression:
Q9Y5S2 MRCKB_HUMAN:

Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.

Sequence:
MSAKVRLKKLEQLLLDGPWRNESALSVETLLDVLVCLYTECSHSALRRDKYVAEFLEWAKPFTQLVKEMQLHREDFEIIKVIGRGAFGEVAVVKMKNTERIYAMKILNKWEMLKRAETACFREERDVLVNGDCQWITALHYAFQDENHLYLVMDYYVGGDLLTLLSKFEDKLPEDMARFYIGEMVLAIDSIHQLHYVHRDIKPDNVLLDVNGHIRLADFGSCLKMNDDGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHEERFQFPSHVTDVSEEAKDLIQRLICSRERRLGQNGIEDFKKHAFFEGLNWENIRNLEAPYIPDVSSPSDTSNFDVDDDVLRNTEILPPGSHTGFSGLHLPFIGFTFTTESCFSDRGSLKSIMQSNTLTKDEDVQRDLEHSLQMEAYERRIRRLEQEKLELSRKLQESTQTVQSLHGSSRALSNSNRDKEIKKLNEEIERLKNKIADSNRLERQLEDTVALRQEREDSTQRLRGLEKQHRVVRQEKEELHKQLVEASERLKSQAKELKDAHQQRKLALQEFSELNERMAELRAQKQKVSRQLRDKEEEMEVATQKVDAMRQEMRRAEKLRKELEAQLDDAVAEASKERKLREHSENFCKQMESELEALKVKQGGRGAGATLEHQQEISKIKSELEKKVLFYEEELVRREASHVLEVKNVKKEVHDSESHQLALQKEILMLKDKLEKSKRERHNEMEEAVGTIKDKYERERAMLFDENKKLTAENEKLCSFVDKLTAQNRQLEDELQDLAAKKESVAHWEAQIAEIIQWVSDEKDARGYLQALASKMTEELEALRSSSLGSRTLDPLWKVRRSQKLDMSARLELQSALEAEIRAKQLVQEELRKVKDANLTLESKLKDSEAKNRELLEEMEILKKKMEEKFRADTGLKLPDFQDSIFEYFNTAPLAHDLTFRTSSASEQETQAPKPEASPSMSVAASEQQEDMARPPQRPSAVPLPTTQALALAGPKPKAHQFSIKSFSSPTQCSHCTSLMVGLIRQGYACEVCSFACHVSCKDGAPQVCPIPPEQSKRPLGVDVQRGIGTAYKGHVKVPKPTGVKKGWQRAYAVVCDCKLFLYDLPEGKSTQPGVIASQVLDLRDDEFSVSSVLASDVIHATRRDIPCIFRVTASLLGAPSKTSSLLILTENENEKRKWVGILEGLQSILHKNRLRNQVVHVPLEAYDSSLPLIKAILTAAIVDADRIAVGLEEGLYVIEVTRDVIVRAADCKKVHQIELAPREKIVILLCGRNHHVHLYPWSSLDGAEGSFDIKLPETKGCQLMATATLKRNSGTCLFVAVKRLILCYEIQRTKPFHRKFNEIVAPGSVQCLAVLRDRLCVGYPSGFCLLSIQGDGQPLNLVNPNDPSLAFLSQQSFDALCAVELESEEYLLCFSHMGLYVDPQGRRARAQELMWPAAPVACSCSPTHVTVYSEYGVDVFDVRTMEWVQTIGLRRIRPLNSEGTLNLLNCEPPRLIYFKSKFSGAVLNVPDTSDNSKKQMLRTRSKRRFVFKVPEEERLQQRREMLRDPELRSKMISNPTNFNHVAHMGPGDGMQVLMDLPLSAVPPSQEERPGPAPTNLARQPPSRNKPYISWPSSGGSEPSVTVPLRSMSDPDQDFDKEPDSDSTKHSTPSNSSNPSGPPSPNSPHRSQLPLEGLEQPACDT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
82
Xenopus
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y5S2 As Substrate

Site PTM Type Enzyme
S304 Phosphorylation
T307 Phosphorylation
S310 Phosphorylation
K314 Ubiquitination
K337 Ubiquitination
S363 Phosphorylation
S417 Phosphorylation
S421 Phosphorylation
T423 Phosphorylation
K426 Ubiquitination
K454 Ubiquitination
K460 Ubiquitination
T467 Phosphorylation
S474 Phosphorylation
S481 Phosphorylation
K489 Acetylation
S524 Phosphorylation
K571 Ubiquitination
S641 Phosphorylation
K642 Ubiquitination
S659 Phosphorylation
K665 Ubiquitination
R671 Methylation
T676 Phosphorylation
S707 Phosphorylation
T757 Phosphorylation
K789 Ubiquitination
K807 Ubiquitination
S840 Phosphorylation
T843 Phosphorylation
S851 Phosphorylation
S852 Phosphorylation
T858 Phosphorylation
K864 Ubiquitination
S868 Phosphorylation
K870 Ubiquitination
K890 Ubiquitination
K910 Ubiquitination
S914 Phosphorylation
K929 Ubiquitination
Y954 Phosphorylation
S1029 Phosphorylation
K1083 Ubiquitination
K1099 Ubiquitination
Y1118 Phosphorylation
K1135 Ubiquitination
T1196 Phosphorylation
K1202 Ubiquitination
K1280 Ubiquitination
K1337 Methylation
K1337 Ubiquitination
Y1355 Phosphorylation
K1526 Methylation
K1528 Methylation
K1528 Ubiquitination
S1530 Phosphorylation
K1544 Ubiquitination
K1559 Ubiquitination
Y1638 Phosphorylation
S1640 Phosphorylation
S1643 Phosphorylation
S1644 Phosphorylation
S1647 Phosphorylation
S1650 Phosphorylation
T1652 Phosphorylation
S1659 Phosphorylation
S1677 Phosphorylation
T1678 Phosphorylation
S1680 Phosphorylation
S1682 Phosphorylation
S1686 Phosphorylation
S1690 Phosphorylation
S1693 Phosphorylation
C1709 S-Nitrosylation

PTMs - Q9Y5S2 As Enzyme

Substrate Site Source
O14974 (PPP1R12A) S852 Uniprot
P24844 (MYL9) S20 Uniprot
P26038 (MSN) T558 Uniprot
P35611 (ADD1) T445 Uniprot

Research Backgrounds

Function:

Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates PPP1R12A. In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity).

PTMs:

Proteolytically cleaved by caspases upon apoptosis induction.

Subcellular Location:

Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction. Cell projection>Lamellipodium.
Note: Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent (By similarity). Detected at the leading edge of migrating cells. Localization at the leading edge of migrating cells requires interaction with catalytically active CDC42 (PubMed:21240187). Localizes in the lamellipodium in a FAM89B/LRAP25-dependent manner (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues examined, with high levels in heart, brain, placenta and lung.

Subunit Structure:

Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42. Interacts with TJP1, when in the presence of catalytically active CDC42 (By similarity). Forms a tripartite complex with MYO18A and LURAP1 with the latter acting as an adapter connecting CDC42BPB and MYO18A. LURAP1 binding results in activation of CDC42BPB by abolition of its negative autoregulation. Interacts with STRIP1, STRN3 and SIKE1. Interacts with CPNE4 (via VWFA domain). Interacts with LURAP1. Interacts (via AGC-kinase C-terminal domain) with FAM89B/LRAP25 (via LRR repeat). Forms a tripartite complex with FAM89B/LRAP25 and LIMK1 (By similarity).

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily.

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