Product: TCFL5 Antibody
Catalog: AF9211
Description: Rabbit polyclonal antibody to TCFL5
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 48kDa; 53kD(Calculated).
Uniprot: Q9UL49
RRID: AB_2843401

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Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Sheep(100%), Rabbit(91%), Dog(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
TCFL5 Antibody detects endogenous levels of total TCFL5.
RRID:
AB_2843401
Cite Format: Affinity Biosciences Cat# AF9211, RRID:AB_2843401.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bHLHe82; CHA; Cha transcription factor; E2BP-1; E2BP1; Figlb; HPV-16 E2-binding protein 1; MGC46135; TCFL 5; TCFL5; TCFL5_HUMAN; transcription factor-like 5 (basic helix-loop-helix); Transcription factor-like 5 protein;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9UL49 TCFL5_HUMAN:

Isoform 3 is testis specific. Isoform 2 is pancreas specific.

Sequence:
MSGPGPREPPPEAGAAGGEAAVEGAGGGDAALGEPGLSFTTTDLSLVEMTEVEYTQLQHILCSHMEAAADGELETRLNSALLAAAGPGAGAGGFAAGGQGGAAPVYPVLCPSALAADAPCLGHIDFQELRMMLLSEAGAAEKTSGGGDGARARADGAAKEGAGAAAAAAGPDGAPEARAKPAVRVRLEDRFNSIPAEPPPAPRGPEPPEPGGALNNLVTLIRHPSELMNVPLQQQNKCTALVKNKTAATTTALQFTYPLFTTNACSTSGNSNLSQTQSSSNSCSVLEAAKHQDIGLPRAFSFCYQQEIESTKQTLGSRNKVLPEQVWIKVGEAALCKQALKRNRSRMRQLDTNVERRALGEIQNVGEGATATQGAWQSSESSQANLGEQAQSGPQGGRSQRRERHNRMERDRRRRIRICCDELNLLVPFCNAETDKATTLQWTTAFLKYIQERHGDSLKKEFESVFCGKTGRRLKLTRPDSLVTCPAQGSLQSSPSMEIK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Chicken
91
Rabbit
91
Horse
0
Bovine
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UL49 As Substrate

Site PTM Type Enzyme
T75 Phosphorylation
T143 Phosphorylation
S144 Phosphorylation
S225 Phosphorylation
S301 Phosphorylation
K320 Ubiquitination
K337 Ubiquitination
S490 Phosphorylation

Research Backgrounds

Function:

Putative transcription factor. Isoform 3 may play a role in early spermatogenesis.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 3 is testis specific. Isoform 2 is pancreas specific.

Subunit Structure:

Efficient DNA binding requires dimerization with another bHLH protein.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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