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$450 200ul

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  • Product Name
    Phospho-PINK1 (Ser228) Antibody
  • Catalog No.
    AF7081
  • RRID
    AB_2843521
  • Source
    Rabbit
  • Application
    WB,IHC,ELISA(peptide)
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig, Bovine, Horse, Sheep, Rabbit, Dog
  • UniProt
  • Mol.Wt
    66kD;
    63kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

BRPK; FLJ27236; mitochondrial; PARK 6; PARK6; Phosphatase and Tensin Homolog; PINK 1; PINK1; PINK1_HUMAN; Protein kinase BRPK; PTEN induced putative kinase 1; PTEN induced putative kinase protein 1; PTEN-induced putative kinase protein 1; Serine/threonine kinase PINK1 mitochondrial; Serine/threonine protein kinase PINK1 mitochondrial; Serine/threonine-protein kinase PINK1;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig, Bovine, Horse, Sheep, Rabbit, Dog

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

Phospho-PINK1 (Ser228) Antibody detects endogenous levels of PINK1 only when phosphorylated at Ser228.

RRID:

AB_2843521
Please cite this product as: Affinity Biosciences Cat# AF7081, RRID:AB_2843521.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human PINK1 around the phosphorylation site of Ser228.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

PINK1

Molecular Weight:

Observed Mol.Wt.: 66kD.
Predicted Mol.Wt.: 63kDa(Calculated)..

Subcellular Location:

Mitochondrion outer membrane. Cytoplasm > cytosol.

Tissue Specificity:

Q9BXM7 PINK1_HUMAN:
Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.

Sequence:
MAVRQALGRGLQLGRALLLRFTGKPGRAYGLGRPGPAAGCVRGERPGWAAGPGAEPRRVGLGLPNRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAVFLAFGLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL

Research Background

Function:

Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PRKN. Targets PRKN to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PRKN in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PRKN and (2) mediating phosphorylation of ubiquitin, converting PRKN to its fully-active form. Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10 (By similarity).

Post-translational Modifications:

Autophosphorylation at Ser-228 and Ser-402 is essential for Parkin/PRKN recruitment to depolarized mitochondria.

Two shorter forms of 55 kDa and 48 kDa seem to be produced by proteolytic cleavage and localize mainly in cytosol. Processed into a 52 kDa mature form by PARL or AFG3L2 following the cleavage by mitochondrial-processing peptidase (MPP) during mitochondrial import.

Subcellular Location:

Mitochondrion outer membrane>Single-pass membrane protein. Mitochondrion inner membrane>Single-pass membrane protein. Cytoplasm>Cytosol.
Note: Localizes mostly in mitochondrion and the 2 proteolytic processed fragments of 55 kDa and 48 kDa localize mainly in cytosol.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.

Subunit Structure:

Interacts with PRKN. Interacts with FBXO7. Forms a complex with PRKN and PARK7. Interacts with NENF.

Similarity:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Research Fields

Research Fields:

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

WB Images(1)


IHC Images(1)


ELISA analysis of AF7081 showing specificity to Phospho-PINK1 (Ser228) Blocking Peptide peptide. Peptides concentration: 1ug/ml.
P-peptide: phospho-peptide; N-peptide: non-phospho-peptide.

Reference Citations:

1). Kato Y;Sakamoto K; et al. Niclosamide affects intracellular TDP-43 distribution in motor neurons, activates mitophagy, and attenuates morphological changes under stress. J Biosci Bioeng 2021 Aug 21;S1389-1723(21)00178-X. (PubMed: 34429248)

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Catalog Number :

AF7081-BP
(Blocking peptide available as AF7081-BP)

Price/Size :

$350/1mg.
Tips: For modified antibodies, we provide modified peptides(0.5mg) and non-modified peptides(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Horse
100%
Bovine
100%
Sheep
100%
Dog
100%
Rabbit
100%
Xenopus
0%
Zebrafish
0%
Chicken
0%
High similarity Medium similarity Low similarity No similarity
Q9BXM7 as Substrate
Site PTM Type Enzyme
K137 Ubiquitination
K164 Ubiquitination
K186 Ubiquitination
S228 Phosphorylation Q9BXM7 (PINK1)
S229 Phosphorylation
S230 Phosphorylation
T257 Phosphorylation Q9BXM7 (PINK1)
K262 Acetylation
S284 Phosphorylation
T313 Phosphorylation Q7KZI7 (MARK2)
K319 Ubiquitination
S402 Phosphorylation Q9BXM7 (PINK1)
K433 Ubiquitination
S465 Phosphorylation
S495 Phosphorylation
K547 Ubiquitination
Q9BXM7 as PTM Enzyme
Substrate Site Source
O15379 (HDAC3) S424 Uniprot
O43464 (HTRA2) S142 Uniprot
O60260 (PRKN) S65 Uniprot
P0CG48 (UBC) S65 Uniprot
Q8IXI2 (RHOT1) S156 Uniprot
Q9BXM7 (PINK1) S228 Uniprot
Q9BXM7 (PINK1) T257 Uniprot
Q9BXM7 (PINK1) S402 Uniprot
Q9Y2N7 (HIF3A) T12 Uniprot
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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