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$450 200ul

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  • Product Name
    Phospho-LATS1 (Thr1079) Antibody
  • Catalog No.
    AF7169
  • RRID
    AB_2843609
  • Source
    Rabbit
  • Application
    WB,IHC,ELISA(peptide)
  • Reactivity
    Human, Mouse, Rat
  • Prediction
    Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
  • UniProt
  • Mol.Wt
    160kD;
    127kDa(Calculated).
  • Concentration
    1mg/ml
  • Browse similar products>>

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Product Information

Alternative Names:Expand▼

h-warts; Large tumor suppressor homolog 1; LATS large tumor suppressor homolog 1; LATS1; LATS1_HUMAN; Serine threonine protein kinase LATS1; Serine/threonine-protein kinase LATS1; WARTS; WARTS protein kinase; wts;

Applications:

WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.

Reactivity:

Human, Mouse, Rat

Predicted Reactivity:

Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus

Source:

Rabbit

Clonality:

Polyclonal

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Specificity:

Phospho-LATS1 (Thr1079) Antibody detects endogenous levels of LATS1 only when phosphorylated at Thr1079.

RRID:

AB_2843609
Please cite this product as: Affinity Biosciences Cat# AF7169, RRID:AB_2843609.

Format:

Liquid

Concentration:

1mg/ml

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Immunogen Information in 3D

Immunogen:

A synthesized peptide derived from human LATS1 around the phosphorylation site of Thr1079.

Uniprot:



>>Visit The Human Protein Atlas

Gene ID:

Gene Name:

LATS1

Molecular Weight:

Observed Mol.Wt.: 160kD.
Predicted Mol.Wt.: 127kDa(Calculated)..

Subcellular Location:

Cytoplasm; cytoskeleton; centrosome.

Tissue Specificity:

O95835 LATS1_HUMAN:
Expressed in all adult tissues examined except for lung and kidney.

Sequence:
MKRSEKPEGYRQMRPKTFPASNYTVSSRQMLQEIRESLRNLSKPSDAAKAEHNMSKMSTEDPRQVRNPPKFGTHHKALQEIRNSLLPFANETNSSRSTSEVNPQMLQDLQAAGFDEDMVIQALQKTNNRSIEAAIEFISKMSYQDPRREQMAAAAARPINASMKPGNVQQSVNRKQSWKGSKESLVPQRHGPPLGESVAYHSESPNSQTDVGRPLSGSGISAFVQAHPSNGQRVNPPPPPQVRSVTPPPPPRGQTPPPRGTTPPPPSWEPNSQTKRYSGNMEYVISRISPVPPGAWQEGYPPPPLNTSPMNPPNQGQRGISSVPVGRQPIIMQSSSKFNFPSGRPGMQNGTGQTDFMIHQNVVPAGTVNRQPPPPYPLTAANGQSPSALQTGGSAAPSSYTNGSIPQSMMVPNRNSHNMELYNISVPGLQTNWPQSSSAPAQSSPSSGHEIPTWQPNIPVRSNSFNNPLGNRASHSANSQPSATTVTAITPAPIQQPVKSMRVLKPELQTALAPTHPSWIPQPIQTVQPSPFPEGTASNVTVMPPVAEAPNYQGPPPPYPKHLLHQNPSVPPYESISKPSKEDQPSLPKEDESEKSYENVDSGDKEKKQITTSPITVRKNKKDEERRESRIQSYSPQAFKFFMEQHVENVLKSHQQRLHRKKQLENEMMRVGLSQDAQDQMRKMLCQKESNYIRLKRAKMDKSMFVKIKTLGIGAFGEVCLARKVDTKALYATKTLRKKDVLLRNQVAHVKAERDILAEADNEWVVRLYYSFQDKDNLYFVMDYIPGGDMMSLLIRMGIFPESLARFYIAELTCAVESVHKMGFIHRDIKPDNILIDRDGHIKLTDFGLCTGFRWTHDSKYYQSGDHPRQDSMDFSNEWGDPSSCRCGDRLKPLERRAARQHQRCLAHSLVGTPNYIAPEVLLRTGYTQLCDWWSVGVILFEMLVGQPPFLAQTPLETQMKVINWQTSLHIPPQAKLSPEASDLIIKLCRGPEDRLGKNGADEIKAHPFFKTIDFSSDLRQQSASYIPKITHPTDTSNFDPVDPDKLWSDDNEEENVNDTLNGWYKNGKHPEHAFYEFTFRRFFDDNGYPYNYPKPIEYEYINSQGSEQQSDEDDQNTGSEIKNRDLVYV

Research Background

Function:

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cells differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1.

Post-translational Modifications:

Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte

Tissue Specificity:

Expressed in all adult tissues examined except for lung and kidney.

Subunit Structure:

Complexes with CDK1 in early mitosis. LATS1-associated CDK1 has no mitotic cyclin partner and no apparent kinase activity. Binds phosphorylated ZYX, locating this protein to the mitotic spindle and suggesting a role for actin regulatory proteins during mitosis. Binds to and colocalizes with LIMK1 at the actomyosin contractile ring during cytokinesis. Interacts (via PPxY motif 2) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA. Interacts with ESR1, DCAF1 and DCAF13; probably recruits DCAF1 and DCAF13 to ESR1 to promote ESR1 ubiquitination and ubiquitin-mediated proteasomal degradation. Interacts with STK3/MST2; this interaction is inhibited in the presence of DLG5. Interacts with SCRIB in the presence of DLG5.

Similarity:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Research Fields

Research Fields:

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.(View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species.(View pathway)

Reference Citations:

1). Zuo W;Liu N;Zeng Y;Xiao Z;Wu K;Yang F;Li B;Song Q;Xiao Y;Liu Q; et al. Luteolin ameliorates experimental pulmonary arterial hypertension via suppressing Hippo-YAP/PI3K/AKT signaling pathway. Front Pharmacol 2021 Apr 15;12:663551. (PubMed: 33935785) [IF=4.225]

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Catalog Number :

AF7169-BP
(Blocking peptide available as AF7169-BP)

Price/Size :

$350/1mg.
Tips: For modified antibodies, we provide modified peptides(0.5mg) and non-modified peptides(0.5mg).

Function :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

Format and storage :

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Precautions :

This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Pig
100%
Horse
100%
Bovine
100%
Sheep
100%
Xenopus
100%
Zebrafish
100%
Chicken
100%
Rabbit
100%
Dog
0%
High similarity Medium similarity Low similarity No similarity
O95835 as Substrate
Site PTM Type Enzyme
T17 Phosphorylation
Y23 Phosphorylation
S26 Phosphorylation
S27 Phosphorylation
S37 Phosphorylation
S42 Phosphorylation
K76 Ubiquitination
S84 Phosphorylation
Y143 Phosphorylation
S171 Phosphorylation
S177 Phosphorylation
S181 Phosphorylation
R243 Methylation
S244 Phosphorylation
T246 Phosphorylation
R252 Methylation
T255 Phosphorylation
R259 Methylation
T261 Phosphorylation
T262 Phosphorylation
K275 Methylation
Y277 Phosphorylation
S278 Phosphorylation
Y283 Phosphorylation
S336 Phosphorylation
S462 Phosphorylation
S464 Phosphorylation O60285 (NUAK1) , Q9H093 (NUAK2)
T490 Phosphorylation P06493 (CDK1)
S580 Phosphorylation
S596 Phosphorylation
T612 Phosphorylation
S613 Phosphorylation P06493 (CDK1)
T616 Phosphorylation
S633 Phosphorylation
S635 Phosphorylation
K662 Ubiquitination
S674 Phosphorylation O95835 (LATS1)
K688 Ubiquitination
K728 Ubiquitination
K734 Ubiquitination
K751 Sumoylation
S792 Phosphorylation
K830 Sumoylation
S864 Phosphorylation
S872 Phosphorylation Q13188 (STK3)
S909 Phosphorylation Q13043 (STK4) , Q13188 (STK3) , O95835 (LATS1)
T967 Phosphorylation Q13188 (STK3)
K976 Ubiquitination
S982 Phosphorylation
K1005 Ubiquitination
T1012 Phosphorylation Q13188 (STK3)
K1029 Ubiquitination
K1046 Ubiquitination
S1049 Phosphorylation O95835 (LATS1)
T1060 Phosphorylation Q13188 (STK3)
Y1076 Phosphorylation
T1079 Phosphorylation Q13043 (STK4) , Q13188 (STK3)
S1107 Phosphorylation
S1111 Phosphorylation
O95835 as PTM Enzyme
Substrate Site Source
O14974 (PPP1R12A) S445 Uniprot
O95835 (LATS1) S674 Uniprot
O95835 (LATS1) S909 Uniprot
O95835 (LATS1) S1049 Uniprot
P04049 (RAF1) S259 Uniprot
P46937-2 (YAP1) S61 Uniprot
P46937-2 (YAP1) S109 Uniprot
P46937 (YAP1) S127 Uniprot
P46937-2 (YAP1) S164 Uniprot
P46937-2 (YAP1) S381 Uniprot
P46937 (YAP1) S397 Uniprot
Q02241 (KIF23) S716 Uniprot
Q02241 (KIF23) S814 Uniprot
Q8NHZ8 (CDC26) T7 Uniprot
Q9GZV5 (WWTR1) S89 Uniprot
Q9NQS7 (INCENP) S894 Uniprot
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

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