Product: Phospho-LATS1 (Thr1079) Antibody
Catalog: AF7169
Description: Rabbit polyclonal antibody to Phospho-LATS1 (Thr1079)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 160kDa; 127kD(Calculated).
Uniprot: O95835
RRID: AB_2843609

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 100ul $350 In stock
 200ul $450 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-LATS1 (Thr1079) Antibody detects endogenous levels of LATS1 only when phosphorylated at Thr1079.
RRID:
AB_2843609
Cite Format: Affinity Biosciences Cat# AF7169, RRID:AB_2843609.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

h-warts; Large tumor suppressor homolog 1; LATS large tumor suppressor homolog 1; LATS1; LATS1_HUMAN; Serine threonine protein kinase LATS1; Serine/threonine-protein kinase LATS1; WARTS; WARTS protein kinase; wts;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O95835 LATS1_HUMAN:

Expressed in all adult tissues examined except for lung and kidney.

Sequence:
MKRSEKPEGYRQMRPKTFPASNYTVSSRQMLQEIRESLRNLSKPSDAAKAEHNMSKMSTEDPRQVRNPPKFGTHHKALQEIRNSLLPFANETNSSRSTSEVNPQMLQDLQAAGFDEDMVIQALQKTNNRSIEAAIEFISKMSYQDPRREQMAAAAARPINASMKPGNVQQSVNRKQSWKGSKESLVPQRHGPPLGESVAYHSESPNSQTDVGRPLSGSGISAFVQAHPSNGQRVNPPPPPQVRSVTPPPPPRGQTPPPRGTTPPPPSWEPNSQTKRYSGNMEYVISRISPVPPGAWQEGYPPPPLNTSPMNPPNQGQRGISSVPVGRQPIIMQSSSKFNFPSGRPGMQNGTGQTDFMIHQNVVPAGTVNRQPPPPYPLTAANGQSPSALQTGGSAAPSSYTNGSIPQSMMVPNRNSHNMELYNISVPGLQTNWPQSSSAPAQSSPSSGHEIPTWQPNIPVRSNSFNNPLGNRASHSANSQPSATTVTAITPAPIQQPVKSMRVLKPELQTALAPTHPSWIPQPIQTVQPSPFPEGTASNVTVMPPVAEAPNYQGPPPPYPKHLLHQNPSVPPYESISKPSKEDQPSLPKEDESEKSYENVDSGDKEKKQITTSPITVRKNKKDEERRESRIQSYSPQAFKFFMEQHVENVLKSHQQRLHRKKQLENEMMRVGLSQDAQDQMRKMLCQKESNYIRLKRAKMDKSMFVKIKTLGIGAFGEVCLARKVDTKALYATKTLRKKDVLLRNQVAHVKAERDILAEADNEWVVRLYYSFQDKDNLYFVMDYIPGGDMMSLLIRMGIFPESLARFYIAELTCAVESVHKMGFIHRDIKPDNILIDRDGHIKLTDFGLCTGFRWTHDSKYYQSGDHPRQDSMDFSNEWGDPSSCRCGDRLKPLERRAARQHQRCLAHSLVGTPNYIAPEVLLRTGYTQLCDWWSVGVILFEMLVGQPPFLAQTPLETQMKVINWQTSLHIPPQAKLSPEASDLIIKLCRGPEDRLGKNGADEIKAHPFFKTIDFSSDLRQQSASYIPKITHPTDTSNFDPVDPDKLWSDDNEEENVNDTLNGWYKNGKHPEHAFYEFTFRRFFDDNGYPYNYPKPIEYEYINSQGSEQQSDEDDQNTGSEIKNRDLVYV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95835 As Substrate

Site PTM Type Enzyme
T17 Phosphorylation
Y23 Phosphorylation
S26 Phosphorylation
S27 Phosphorylation
S37 Phosphorylation
S42 Phosphorylation
K76 Ubiquitination
S84 Phosphorylation
Y143 Phosphorylation
S171 Phosphorylation
S177 Phosphorylation
S181 Phosphorylation
R243 Methylation
S244 Phosphorylation
T246 Phosphorylation
R252 Methylation
T255 Phosphorylation
R259 Methylation
T261 Phosphorylation
T262 Phosphorylation
K275 Methylation
Y277 Phosphorylation
S278 Phosphorylation
Y283 Phosphorylation
S336 Phosphorylation
S462 Phosphorylation
S464 Phosphorylation O60285 (NUAK1) , Q9H093 (NUAK2)
T490 Phosphorylation P06493 (CDK1)
S580 Phosphorylation
S596 Phosphorylation
T612 Phosphorylation
S613 Phosphorylation P06493 (CDK1)
T616 Phosphorylation
S633 Phosphorylation
S635 Phosphorylation
K662 Ubiquitination
S674 Phosphorylation O95835 (LATS1)
K688 Ubiquitination
K728 Ubiquitination
K734 Ubiquitination
K751 Sumoylation
S792 Phosphorylation
K830 Sumoylation
S864 Phosphorylation
S872 Phosphorylation Q13188 (STK3)
S909 Phosphorylation Q13043 (STK4) , Q13188 (STK3) , O95835 (LATS1)
T967 Phosphorylation Q13188 (STK3)
K976 Ubiquitination
S982 Phosphorylation
K1005 Ubiquitination
T1012 Phosphorylation Q13188 (STK3)
K1029 Ubiquitination
K1046 Ubiquitination
S1049 Phosphorylation O95835 (LATS1)
T1060 Phosphorylation Q13188 (STK3)
Y1076 Phosphorylation
T1079 Phosphorylation Q13043 (STK4) , Q13188 (STK3)
S1107 Phosphorylation
S1111 Phosphorylation

PTMs - O95835 As Enzyme

Substrate Site Source
O14974 (PPP1R12A) S445 Uniprot
O95835 (LATS1) S674 Uniprot
O95835 (LATS1) S909 Uniprot
O95835 (LATS1) S1049 Uniprot
P04049 (RAF1) S259 Uniprot
P46937-2 (YAP1) S61 Uniprot
P46937-2 (YAP1) S109 Uniprot
P46937 (YAP1) S127 Uniprot
P46937-2 (YAP1) S164 Uniprot
P46937-2 (YAP1) S381 Uniprot
P46937 (YAP1) S397 Uniprot
Q02241 (KIF23) S716 Uniprot
Q02241 (KIF23) S814 Uniprot
Q8NHZ8 (CDC26) T7 Uniprot
Q9GZV5 (WWTR1) S89 Uniprot
Q9NQS7 (INCENP) S894 Uniprot

Research Backgrounds

Function:

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cells differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1.

PTMs:

Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all adult tissues examined except for lung and kidney.

Subunit Structure:

Complexes with CDK1 in early mitosis. LATS1-associated CDK1 has no mitotic cyclin partner and no apparent kinase activity. Binds phosphorylated ZYX, locating this protein to the mitotic spindle and suggesting a role for actin regulatory proteins during mitosis. Binds to and colocalizes with LIMK1 at the actomyosin contractile ring during cytokinesis. Interacts (via PPxY motif 2) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA. Interacts with ESR1, DCAF1 and DCAF13; probably recruits DCAF1 and DCAF13 to ESR1 to promote ESR1 ubiquitination and ubiquitin-mediated proteasomal degradation. Interacts with STK3/MST2; this interaction is inhibited in the presence of DLG5. Interacts with SCRIB in the presence of DLG5.

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Research Fields

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species.   (View pathway)

References

1). Luteolin ameliorates experimental pulmonary arterial hypertension via suppressing Hippo-YAP/PI3K/AKT signaling pathway. Frontiers in Pharmacology (PubMed: 33935785) [IF=5.6]

Application: WB    Species: rat    Sample: muscle cell

FIGURE 5 Luteolin inhibits the increase of mRNA and protein expression of key molecules in Hippo/YAP signaling pathway induced by PDGF-BB. PASMCs were cultured in 1% serum medium for 24 h, then treated with different concentrations of luteolin for 1 h before the stimulation with PDGF-BB (20 ng/ml). (A) mRNA level of LATS1 (n = 3). (B) mRNA level of YAP (n = 3). (C) and (D) the expression of protein level of LATS1 and phosphorylated LATS1 (n = 5). (E) and (F) the expression of protein level of YAP and phosphorylated YAP (n = 3). Bars represent means ± SD of n = 3–6, #DMSO group vs PDGF + DMSO group, #p < 0.05, ##p < 0.01, ###p < 0.0001, ###p < 0.0001, ####p < 0.00001, *PDGF + DMSO group vs PDGF + LUT group, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, NS indicates no significance. LATS1, large tumor suppressor one; LUT, luteolin; PASMCs, pulmonary arterial smooth muscle cells; PDGF-BB, platelet-derived growth factor-BB; YAP, yes-association protein.

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