Phospho-GATA3 (Ser369) Antibody - AF7271
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- Product Name
- Phospho-GATA3 (Ser369) Antibody
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- Catalog No.
- AF7271
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- RRID
- AB_2843711
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- Source
- Rabbit
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- Application
- WB,IF/ICC,ELISA
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- Reactivity
- Human, Mouse
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- Prediction
- Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
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- UniProt
-
- Mol.Wt
- 48kD;
48kDa(Calculated). -
- Concentration
- 1mg/ml
Related Products
Product Information
Alternative Names:Expand▼
GATA 3; GATA binding factor 3; GATA binding protein 3; GATA-binding factor 3; Gata3; GATA3_HUMAN; HDR; HDRS; MGC2346; MGC5199; MGC5445; Trans acting T cell specific transcription factor GATA 3; Trans-acting T-cell-specific transcription factor GATA-3;
Applications:
WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
Human, Mouse
Predicted Reactivity:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Source:
Rabbit
Clonality:
Polyclonal
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Specificity:
Phospho-GATA3 (Ser369) Antibody detects endogenous levels of GATA3 only when phosphorylated at Ser369.
RRID:
AB_2843711
Please cite this product as: Affinity Biosciences Cat# AF7271, RRID:AB_2843711.
Format:
Liquid
Concentration:
1mg/ml
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Immunogen Information
Immunogen:
A synthesized peptide derived from human GATA3 around the phosphorylation site of Ser369.
Uniprot:
>>Visit The Human Protein Atlas
Gene ID:
Gene Name:
GATA3
Molecular Weight:
Observed Mol.Wt.: 48kD.
Predicted Mol.Wt.: 48kDa(Calculated)..
Subcellular Location:
Nucleus.
Tissue Specificity:
P23771 GATA3_HUMAN:
T-cells and endothelial cells.
Sequence:
- P23771 GATA3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEVTADQPRWVSHHHPAVLNGQHPDTHHPGLSHSYMDAAQYPLPEEVDVLFNIDGQGNHVPPYYGNSVRATVQRYPPTHHGSQVCRPPLLHGSLPWLDGGKALGSHHTASPWNLSPFSKTSIHHGSPGPLSVYPPASSSSLSGGHASPHLFTFPPTPPKDVSPDPSLSTPGSAGSARQDEKECLKYQVPLPDSMKLESSHSRGSMTALGGASSSTHHPITTYPPYVPEYSSGLFPPSSLLGGSPTGFGCKSRPKARSSTGRECVNCGATSTPLWRRDGTGHYLCNACGLYHKMNGQNRPLIKPKRRLSAARRAGTSCANCQTTTTTLWRRNANGDPVCNACGLYYKLHNINRPLTMKKEGIQTRNRKMSSKSKKCKKVHDSLEDFPKNSSFNPAALSRHMSSLSHISPFSHSSHMLTTPTPMHPPSSLSFGPHHPSSMVTAMG
Research Background
Function:
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses.
Subcellular Location:
Nucleus.
Tissue Specificity:
T-cells and endothelial cells.
Subunit Structure:
Interacts with TBX21 ('Tyr-530' phosphorylated form).
Similarity:
Binds DNA via the 2 GATA-type zinc fingers. Each zinc finger may bind either adjacent sites in a palindromic motif, or a different DNA molecule allowing looping and long-range gene regulation.
The YxKxHxxxRP motif is critical for DNA-binding and function.
Research Fields
Research Fields:
· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.(View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation.(View pathway)
Protocols & Troubleshooting
WB Images(1)
IF/ICC Images(1)
P-peptide: phospho-peptide; N-peptide: non-phospho-peptide.
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Catalog Number :
AF7271-BP
(Blocking peptide available as AF7271-BP)
Price/Size :
$350/1mg.
Tips: For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).
Function :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.
Format and storage :
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Precautions :
This product is for research use only. Not for use in diagnostic or therapeutic procedures.
P23771 as Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| S110 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| T156 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S162 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| Y186 | Phosphorylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| R261 | Methylation | Uniprot | |
| T271 | Phosphorylation | Uniprot | |
| Y282 | Phosphorylation | Uniprot | |
| Y290 | Phosphorylation | Uniprot | |
| K292 | Acetylation | Uniprot | |
| K304 | Acetylation | Uniprot | |
| S308 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| T315 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| Y344 | Phosphorylation | Uniprot | |
| K346 | Acetylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| K371 | Acetylation | Uniprot | |
| K373 | Acetylation | Uniprot | |
| K374 | Acetylation | Uniprot | |
| K376 | Acetylation | Uniprot | |
| K377 | Ubiquitination | Uniprot | |
| S381 | Phosphorylation | Uniprot |
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